2004 Fiscal Year Final Research Report Summary
Investigation on the mechanism of chronic rejection in organ transplantation and the development of a new modality evaluating the chronic rejection.
| Project/Area Number |
14571154
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Saitama Medical University |
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Principal Investigator |
KOYAMA Isamu Saitama Medical School, Faculty of Medicine, Professor, 医学部, 教授 (60178390)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAZAWA Mitsuo Saitama Medical School, Faculty of Medicine, 医学部, 助教授 (20200165)
OGAWA Nobuji Saitama Medical School, Faculty of Medicine, Assistant Professor, 医学部, 講師 (60286044)
TAKAHASHI Kouichi Saitama Medical School, Faculty of Medicine, Fellow, 医学部, 助手 (40306311)
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| Project Period (FY) |
2002 – 2004
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| Keywords | Chronic rejection / Immunological status / TGF-β / MMP-2 / Immunosuppressive acidic protein / Nitric Oxide |
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| Research Abstract |
The pathological findings of an end stage chronic rejection are the fibrosis of the interstitial space, resulting in the atrophy of the organs. Various kinds of growth factor might be associated with the process of the fibrosis. Among of them, we focused TGF-β and MMP-2 which have thought to be most related to the tissue fibrosis, and we examined in the first whether or not these two growth factor or cytokine would help to predict the development of chronic rejection. Moreover, it was studied whether immunosuppressive acid proteins (IAP) and NO (nitric oxide) help to evaluate the immunological status in the chronic stage after renal transplantation. The TGF-β value was elevated in the patients with poor renal function in the late stage after renal transplantation. However, the doubt remained about utility of the TGF-β as the marker of the diagnosis at the early stage of a chronic rejection. As for MMP-2, a significant difference was not seen between the normal kidney function group and the poor kidney function group. In addition, it was suggested that serum Cr level of 3mg/dl and serum NO value of 150μmol/L might be able to predict the renal graft lost within one year, which turned out the NO measurement also help to diagnose the early stage of the chronic period rejection. The monitoring of the immunological status after transplantation is very important for obtaining an appropriate immunosuppression, which may help to prevent from chronic rejection. Differential diagnosis between chronic rejection and viral infection was difficult by only the measurement of IAP, although serum IAP value was useful as the immunological monitoring. We developed a new method of immunological monitoring after transplantation in which the patients' lymphocytes were analyzed with FACS for DNA pattern under the stimulation with a super antigen. This new method might be helpful to predict the development of chronic rejection in future.
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