2003 Fiscal Year Final Research Report Summary
Mechanism of latent infection of Herpesviridae and its reactivation following organ transplantation
| Project/Area Number |
14571159
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Tokai University |
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Principal Investigator |
TANAKA Kazuo Tokai University, School of Medicine, Associate Professor, 医学部, 助教授 (50236569)
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| Co-Investigator(Kenkyū-buntansha) |
SAWAMURA Sadaki Tokai University, School of Medicine, Assistant Researcher, 医学部, 助手 (80056241)
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| Project Period (FY) |
2002 – 2003
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| Keywords | cytomegalovirus / real-time PCR / infectious immunity / memory cells / MHC / tetramer / germfree mouse / LPS / CpG motif |
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| Research Abstract |
BALB/c mice were ip infected with murine cytomegalovirus (MCMV). When virus titers were enumerated by real-time PCR, it was demonstrated that the virus was latently infected in the lungs 3 months after the infection. However, a large number (Ca.10% of total pulmonary lymphocytes) of MCMV-specific memory-effctor CTL, which was examined using MHC/antigen-tetramer, was still detectable in the organs even 1 year after the infection. Thus, MCMV-specific memory-effctor CTL was presumably maintained without MCMV antigens. Thus, MCMV-specific memory-effctor CTL may play a role in prevention of CMV-reactivation.
On the other hand, such MCMV-specific memory-effctor CTL could not be detected in the lungs of germfree mice, suggesting that such bacterial product as LPS and/or CpG motif may participate in the maintenace of MCMV-specific memory-effctor CTL. This result suggested that eradication of intestinal bacterial flora in pre-treatment before bone marrow transplantation may diminish indigenous MCMV-specific memory-effctor CTL.
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