2005 Fiscal Year Final Research Report Summary

Potential of primary care of multiple organ failure by gene analyses of characteristics of enterotoxin and endotoxin production in mixed infection of MTRSA and E. coli and fundamental examination

Research Project

Project/Area Number	14571237
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Digestive surgery
Research Institution	Toho University
Principal Investigator	SUMIYAMA Y Toho University, Faculty of Medicine, Professor, 医学部, 教授 (10057648)
Co-Investigator(Kenkyū-buntansha)	KUSACHI S Toho University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (70169978) ARIMA Y Toho University, Faculty of Medicine, Instructor, 医学部, 助手 (50277290) YOSHIDA Y Toho University, Faculty of Medicine, Instructor, 医学部, 助手 (10318298) TANAKA H Toho University, Faculty of Medicine, Instructor, 医学部, 助手 (80307735) YOICHI Y Toho University, Faculty of Medicine, Instructor, 医学部, 助手 (10349904)
Project Period (FY)	2002 – 2005
Keywords	MRSA / sepsis / mouse / anti TSST-1 antibody / IL-1 / IFN-γ / bacterialtranslocation / TSST-1
Research Abstract	We made gut-derived sepsis with MRSA in mice. The bacterial suspension was orally administered between days 1 and 3. To aid the colonization of MRSA, the normal intestinal flora of the mice was disturbed by administering ampicillin (ABPC) by subcutaneous injection daily between days 1 and 3. After the administration of bacteria, mice were then given 250 mg/kg of cyclophosphamide (CY) by intraperitoneal injection on day 1, 4 and 7. We studied the efficacy of antiTSST-1 monoclonal antibody against gut-derived sepsis with MRSA. Although the significant difference was not recognized, antiTSST-1 monoclonal antibody administrated group tended to show highly survival rate. We also studied about host defense. WT mice, IL-1 KO and IFN-γ KO mice were used in gut-derived sepsis with MRSA. Although WT mice were survived more than 80%, IL-1 KO mice died all. The numbers of viable bacteria in organs were significantly high level in the IL-1 KO mice. IFN-γ KO mice were tend to die than WT mice. IL-1 and IFN-γ were indicated that they were one of important host defense cytokines.