2003 Fiscal Year Final Research Report Summary
Experimental therapy for malignant brain tumors using viral vector and neuronal stem cell
| Project/Area Number |
14571312
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
ONO Yasuhiro OKAYAMA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE AND DENTISTRY, LECTURER, 大学院・医歯学総合研究科, 講師 (40294409)
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| Co-Investigator(Kenkyū-buntansha) |
ONO Shigeki OKAYAMA UNIVERSITY, HOSPITALS, ASSISTANT, 医学部・歯学部附属病院, 助手 (40335625)
TOKUNAGA Kouji OKAYAMA UNIVERSITY, HOSPITALS, LECTURER, 医学部・歯学部附属病院, 講師 (40294467)
SUGIU Kenji OKAYAMA UNIVERSITY, HOSPITALS, LECTURER, 医学部・歯学部附属病院, 講師 (40325105)
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| Project Period (FY) |
2002 – 2003
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| Keywords | BRAIN TUMORS / GENE TNERAPY / NEURONAL STEMM CELL / VIRAL VECTOR |
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| Research Abstract |
We have investigated the feasibility of gene transduction for braintumors using adenovirus vector. Transduction of the cytosine deaminase gene into tumor cells followed by administration of 5-fluorocytosine was found to have strong antitumor effect against experimental malignant brain tumor in vitro and in vivo. We also found its toxicity and limited antitumor effect on infiltrating tumor cells in normal brain tissue which cause local tumor recurrence. The purpose of this research was to improve antitumor effect on infiltrating tumor cells within normal brain tissue while minimizing neurotoxicity on normal brain cells. We developed new method to attain high local concentration of toxic drug coverted by CD transduced cell. We employed neuronal stem cells and bone marrow stem cells which harbour therapeutic gene and track infiltrating tumor cells. We showed their therapeutic effect in vitro when cultured with glioma cells. Then we investigated tracking of stem cells to infiltrating tumor cell in rat brain tumor model. We are now investigating in vivo therapeutic effect using stern cells harboring therapeutic gene.
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[Publications] Terada K, Tamiya T, Daido S, Kambara H, Tanaka H, Ono Y, Matsumoto K, Ito S, Ouchida M, Ohmoto T, Shimizu K.: "Prognostic value of loss of heterozygosity around three candidate tumor suppressor genes on chromosome 10q in astrocytomas."J Neurooncol. 58. 107-114 (2002)
Description
「研究成果報告書概要(欧文)」より
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