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2004 Fiscal Year Final Research Report Summary

The signaling and transmission of pain and synaptic plasticity : in vivo patch clamp analysis of synaptic transmission at dorsal horn neurons in the rat spinal cord.

Research Project

Project/Area Number 14571420
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionGunma University

Principal Investigator

NISHIKAWA Koichi  Gunma University, School of medicine, Assistant Professor, 医学部, 講師 (00334110)

Project Period (FY) 2002 – 2004
KeywordsGABA receptor / volatile anesthetics / patch-clamp method / synaptic transmission / synaptic plasticity / pain / dorsal horn / neuropathic pain
Research Abstract

This purpose of the present project was to investigate the interactions of noradrenaline and sevoflurane on inhibitory synaptic transmission mediated by GABA(A) receptors in the rat hippocampus. Pharmacologically isolated GABA(A) receptor-mediated IPSCs were recordedwith whole-cell patch clamp techniques in pyramidal neurons of the CA1 region of rat hippocampal slices. The actions of noradrenaline, noradrenaline analog, sevoflurane, and the interactions of these agents on the frequency and kinetics of spontaneous GABA(A) receptor-mediated IPSCs were studied. Noradrenaline (10 μM) caused an increase in the frequency of action potential-dependent sIPSCs. These effects were completely reversed by the addition of tetrodotoxin (1 μM), suggesting that noradrenaline produces the discharge of GABAergic interneurons innervating on pyramidal cells via adrenoceptors. Although sevoflurane (0.40 mM, 20 min) slightly depressed the amplitude of sIPSCs, sevoflurane significantly prolonged the decay ti … More me constant to 451.1±89.0% of control (n=9,P<0.001) without affecting the rise time. In addition, sevoflurane increased the frequency of sIPSCs up to three folds. However, pretreatment of cadmium, multiple Ca channel blocker, abolished sevoflurane effects on the frequency whereas the effects on the decay was still observed. Application of both noradrenaline and sevoflurane produced the significant increase of the IPSC frequency than that of noradrenaline alone or sevoflurane alone with prolonged decays. These results provide evidence that both agents have additive effects on GABAergic synaptic transmission at the central nervous system via different mechanisms.
We also investigated the effects of propofol on excitatory and inhibitory postsynaptic currents (EPSCs and IPSCs) and voltage responses in SG neurons by mechanical (pinch) stimuli applied to the skin using the newly developed in vivo patch-clamp technique. We found that propofol significantly prolonged the decay of IPSCs and decreased the frequency without affecting excitatory synaptic responses. In addition, propofol appeared to depress excitability of SG neurons. Less

  • Research Products

    (12 results)

All 2005 2004 2003 2002

All Journal Article (10 results) Book (2 results)

  • [Journal Article] The interaction of noradrenaline with sevoflurane on GABA(A) receptor-mediated inhibitory postsynaptic currents in the rat hippocampus.2005

    • Author(s)
      Nishikawa K, Kubo K, Ishizeki J, Takazawa T, Saito S, Goto F
    • Journal Title

      Brain Research 1039

      Pages: 153-161

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Volatile anesthetics inhibit calcitonin gene-related peptide (CGRP) receptor-mediated responses in pithed rats and human neuroblastoma cells.2004

    • Author(s)
      Kuroda M, Yoshikawa D, Nishikawa K, Saito S, Goto F
    • Journal Title

      Journal of Pharmacology and Experimental Therapeutics 311

      Pages: 1016-1022

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 揮発性麻酔薬と中枢神経シナプス伝達2004

    • Author(s)
      西川 光一
    • Journal Title

      麻酔 53

      Pages: 873-881

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] モルヒネ耐性のメカニズムと耐性克服のための実験的アプローチ2004

    • Author(s)
      西川 光一, 田野辺 恭子, 日野原 宏, 岡本 知紀, 斎藤 繁, 後藤 文夫
    • Journal Title

      麻酔 53

      Pages: 502-507

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 吸入麻酔薬と中枢神経系抑制シナプス伝達-GABA(A)受容体への麻酔作用と介在神経細胞の役割2004

    • Author(s)
      西川 光一
    • Journal Title

      日本麻酔薬理学会誌 16

      Pages: 19-22

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Volatile anesthetics inhibit calcitonin gene-related peptide(CGRP) receptor-mediated responses in pithed rats and human neuroblastoma cells.2004

    • Author(s)
      Kuroda M, Yoshikawa D, Nishikawa K, Saito S, Goto F
    • Journal Title

      Journal of Pharmacology and Experimental Therapeutics 311

      Pages: 1016-1022

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The actions of sevoflurane and desflurane on the gamma-aminobutyric acid receptor type A : effects of TM2 mutations in the alpha and beta subunits.2003

    • Author(s)
      Nishikawa K, Harrison NL
    • Journal Title

      Anesthesiology 99

      Pages: 678-684

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 血液脳関門と全身麻酔薬.2003

    • Author(s)
      田野辺恭子, 西川光一, 日野原宏, 岡本知紀, 斎藤繁, 後藤文夫
    • Journal Title

      麻酔 52

      Pages: 840-845

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 全身麻酔薬の作用部位としてのイオンチャネル.2002

    • Author(s)
      西川 光一, 後藤文夫
    • Journal Title

      臨床麻酔 26

      Pages: 785-790

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 静脈麻酔薬の分子作用機構.2002

    • Author(s)
      西川 光一, 富岡 昭裕, 齋藤 繁, 後藤 文夫
    • Journal Title

      臨床麻酔 26

      Pages: 1176-1784

    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 全身麻酔薬の中枢神経作用 麻酔科レビュー2005-最新主要文献集-(天羽敬祐編)2005

    • Author(s)
      西川 光一
    • Total Pages
      5
    • Publisher
      総合医学社
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 全身麻酔薬の中枢神経作用 麻酔科レビュー2004-最新主要文献集-(天羽敬祐編)2004

    • Author(s)
      西川 光一
    • Total Pages
      4
    • Publisher
      総合医学社
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2006-07-11  

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