2004 Fiscal Year Final Research Report Summary
Investigation of inhibitors in crystal-cell injury of renal tubular epithelium by oxalate and calcium oxalate
| Project/Area Number |
14571499
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Osaka University |
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Principal Investigator |
YOSHIMURA Kazuhiro Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 講師 (20283757)
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| Project Period (FY) |
2002 – 2004
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| Keywords | Urolithiasis / Calcium oxalate / HGF / Fibronectin |
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| Research Abstract |
(1) OJECTIVE : We investigated whether HGF has a role on apoptosis and crystal-cell interaction and we investigated protective effects of HGF gene therapy on stone forming rat model using electroporation -mediated gene transfer. (2) METHODS : MDCK cells were exposed to potassium oxalate or COM crystals in the presence or absence of HGF (0 to 100 ng/ml). We measured the lactate dehydrogenase (LDH) activity into the medium as a marker of cell injury and the effects of HGF on apoptosis were analyzed by flow cytometry. We examined the inhibitory effect of HGF and fibronectin on the adhesion of COM crystals to MDCK cells. (3) RESULTS : Exposure of both KOX and COM crystals to MDCK cells resulted in an increase in LDH release and apoptotic cells, but these effects were reduced by HGF and finbonectin. Both HGF and fibronectin had inhibitory activities against the adhesion of COM crystals to MDCK cells. (4) CONCLUSIONS : HGF and finronectin have inhibitory effects in cell injuries by high oxalate level and against the adhesion of COM crystals to MDCK cells.
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