2003 Fiscal Year Final Research Report Summary
Comparative analysis of immune cells participating in acute and chronic rejection of kidney allografts.
| Project/Area Number |
14571520
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | KITASATO UNIVERSITY |
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Principal Investigator |
OBATA Fumiya Kitasato University, School of Allied Health Sciences, Professor, 医療衛生学部, 教授 (60129236)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Kazunari Kitasato University, School of Medicine, Instructor, 医学部, 講師 (10174921)
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| Project Period (FY) |
2002 – 2003
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| Keywords | kidney transplantation / acute rejection / chronic rejection / cytokines / Th1 / Th2 / IFN-γ / IL-4 / IL-10 |
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| Research Abstract |
T cells mediating chronic rejection (CR) of human kidney allografts were characterized by comparing them with those mediating acute rejection (AR). Two lines of analysis were performed using biopsy specimens (23 CR and 8 AR). First, the extent of infiltration of CD4^+ and CD8^+T cells into allografts was assessed from mRNA expression of CD4 and CD8. The group of CR specimens was not significantly different from the group of AR specimens in terms of the extent of CD4^+ and CD8^+T-cell infiltration, underlining the importance of the immunological contribution to the progress of CR. Second, Th1/Th2 polarization in infiltrating T cells was investigated by measuring mRNA expression of interferon gamma (IFN-γ, a Th1 cytokine) and interleukin 4 (IL-4, a Th2 cytokine). IFN-γ expression was detected in most CR specimens, and was not significantly different between the group of CR specimens and the group of AR specimens. On the other hand, IL-4 expression was detected in only two CR specimens and one AR specimen ; from its pathological features, the AR in this last case was concomitant with CR. These results suggest that most cases of CR and of AR are mediated by Th1 mechanisms, although some cases of CR show features of both Th1 and Th2.
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Research Products
(4 results)
