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2003 Fiscal Year Final Research Report Summary

Comparative analysis of immune cells participating in acute and chronic rejection of kidney allografts.

Research Project

Project/Area Number 14571520
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionKITASATO UNIVERSITY

Principal Investigator

OBATA Fumiya  Kitasato University, School of Allied Health Sciences, Professor, 医療衛生学部, 教授 (60129236)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Kazunari  Kitasato University, School of Medicine, Instructor, 医学部, 講師 (10174921)
Project Period (FY) 2002 – 2003
Keywordskidney transplantation / acute rejection / chronic rejection / cytokines / Th1 / Th2 / IFN-γ / IL-4 / IL-10
Research Abstract

T cells mediating chronic rejection (CR) of human kidney allografts were characterized by comparing them with those mediating acute rejection (AR). Two lines of analysis were performed using biopsy specimens (23 CR and 8 AR). First, the extent of infiltration of CD4^+ and CD8^+T cells into allografts was assessed from mRNA expression of CD4 and CD8. The group of CR specimens was not significantly different from the group of AR specimens in terms of the extent of CD4^+ and CD8^+T-cell infiltration, underlining the importance of the immunological contribution to the progress of CR. Second, Th1/Th2 polarization in infiltrating T cells was investigated by measuring mRNA expression of interferon gamma (IFN-γ, a Th1 cytokine) and interleukin 4 (IL-4, a Th2 cytokine). IFN-γ expression was detected in most CR specimens, and was not significantly different between the group of CR specimens and the group of AR specimens. On the other hand, IL-4 expression was detected in only two CR specimens and one AR specimen ; from its pathological features, the AR in this last case was concomitant with CR. These results suggest that most cases of CR and of AR are mediated by Th1 mechanisms, although some cases of CR show features of both Th1 and Th2.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Komatsu H: "Oxidative modulation of the glutathione-redox couple enhance lipopolysaccharide-induced interleukin 12 p40 production by a mouse macrophage cell line, J774A.1."Free Radical Res. 37(3). 293-299 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Komatsu H: "An optimized method for determination of intracellular glutathione in mouse macrophage cultured by fluorometric high-performance liquid chromatography."Biomedical Chromatography. 17(5). 345-350 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Komatsu H, Hoshino A, Funayama M, Kawahara K, Obata F.: "Oxidative modulation of the glutathione-redox couple enhance lipopolysaccharide-induced interleukin 12 p40 production by a mouse macrophage cell line, J774A.1."Free Radical Res. 37(3). 293-299 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Komatsu H., Obata F.: "An optimized method for determination of intracellular glutathione in mouse macrophage cultures by fluorometric high-performance liquid chromatography."Biomed Chromatogr. 17(5). 345-350 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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