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2003 Fiscal Year Final Research Report Summary

Molecular analysis of reproductive fanction on the genes implicated to sex differentiation and gametogenesis

Research Project

Project/Area Number 14571583
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKEIO UNIVERSITY

Principal Investigator

SUEOKA Kou  Keio Univ., School of Medicine, Associate Professor, 医学部, 助教授 (90162833)

Co-Investigator(Kenkyū-buntansha) IWATA Soukichi  Keio Univ., School of Medicine, Instructor, 医学部, 助手 (50203386)
KUJI Naoaki  Keio Univ., School of Medicine, Assistant Professor, 医学部, 講師 (80169987)
YOSHIMURA Yasunori  Keio Univ., School of Medicine, Professor, 医学部, 教授 (10129736)
MAEDA Taro  Keio Univ., School of Medicine, Instructor, 医学部, 助手 (90327594)
TAJIMA Hiroto  Keio Univ., School of Medicine, Instructor, 医学部, 助手 (30317193)
Project Period (FY) 2002 – 2003
Keywordsspermatogenesis / doxorubicin / c-kit / F-TRAP / telomerase / TUNEL / green tea extract (GTE) / apoptosis
Research Abstract

The implication of c-kit was examined on the aspect of gene expression and c-kit production at testicular germ cell by RT-PCR and immunohistochemistry in the mice testes damaged by doxorubicin (DXR) as a germ cell toxic model. And also the promoting effect of herbal medicine (TJ41, TJ7) and GTCs on telomerase activity and apoptosis were investigated. Apoptotic cells were labelled by the TdT-mediated dUDP nick-end labelling (TUNEL) assay. The weight of testes was significantly decreased to 55-64% by DXR exposure, while the combined treatment of GTCs with DXR resulted in parameters similar to the control. In the testes of DXR-exposed mice, germ cells in the testicular tubules of control were drastically reduced by about 80% by histological analysis. However, germ cell damage was significantly attenuated by TJ41 and GTCs coadministration. c-Kit and its gene expression were reduced in the DXR exposured testis, but not in the group with promoting agents. Although the telomerase activity evaluated by fluorescence-based TRAP assay was increased with coadministration of TJ41 and GTCs compared with control, that was decreased by DXR expoosure without these promoting agents. The promoting agents with DXR show a marked reduction in the number of apoptotic cells compared with DXR exposure without these agents. These results suggest that testicular dysfunction following exposure of toxic chemicals are determining at the mechanisms provoked by c-kit and its gene expression and TJ41 and GTCs may have eventful evidence of a protective effect against DXR-induced testicular toxicity via promoting telomerase activity by inhibition of testicular apoptosis.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 末岡 浩: "着床前診断-各種遺伝子型に対する技術開発"医学のあゆみ. 209(7). 431-432 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 末岡 浩: "遺伝子病の着床前診断(PGD)"産婦人科の世界. 56. 264-270 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田島博人: "着床前診断を前提としたrealtime PCR法による点変異型および1塩基挿入型dystrophin遺伝子の検出限界"日本受精着床学会雑誌. 21(1). 35-40 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷垣礼子: "造精機能障害モデルマウスにおける分化因子の動向と漢方薬の劇的効果"産婦人科 漢方研究のあゆみ. 20. 83-88 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Nakabayashi: "Incidental deviation of short and long CAG repeats in the androgen receptor gene for Japanese male infertility."Reproductive Medicine and Biology. 2(4). 145-150 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kou Sueoka: "Preimplantation genetic diagnosis -the technological development for various genetic types."Journal of Clinical and Experimental Medicine. 209(7). 431-432 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kou Sueoka, Hiroto Tajima, Akira Nakabayashi, Taro Maeda, Hiroyoshi Watanabe, Tuyoshi Hashiba, Yasunori Yoshimura: "Preimplantation diagnosis for genetic diseases (PGD)."The World of Obstetrics & Gynecology. 56. 264-270 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] HIroto Tajima, Kou Sueoka, Taro Maeda, Kenji Satoh, Akira Nakabayashi, Tsuyoshi Hashiba, Soukichi Iwata, Naoaki Kuji, Yasunori Yoshimura: "Advantages and limits in the detection of point mutation and single base insertion by realtime PCR for preimplantation genetic diagnosis."Journal of Fertilization and Implantation. 21(1). 35-40 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Reiko Tanigaki, Kou Sueoka, Hiroto Tajima, Kenji Satoh, Akira Nakabayashi, Naoaki Kuji, Yasunori Yoshimura, Shiro Nozawa, Shingo Katoh, Yoshihiko Hosoi: "The changes of differentiation implicated factors on spermatogenic arrest model mouse and the drastic effect of herbal medicine."Recent progress of KAMPO MEDICINE in obstetrics and gynecology. 20. 83-88 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akira Nakabayashi, Kou Sueoka, Noriko Matsuda, Hironori Asada, Reiko Tanigaki, Kenji Satoh, Hiroto Tajima, Tsutomu Ogata, Naoaki Kuji, Yasunori Yoshimura: "Incidental deviation of short and long CAG repeats in the androgen receptor gene for Japanese male infertility."Reproductive Medicine and Biology. 2. 145-150 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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