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2003 Fiscal Year Final Research Report Summary

Investigation of changes in cell properties and resistance to anticancer drugs as a result of transfection of H-type sugar chain genes into ovarian cancer cells

Research Project

Project/Area Number 14571585
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKEIO UNIVERSITY

Principal Investigator

KUBUSHIRO Kaneyuki  Keio University, school of Medicine, Assistant Professor, 医学部, 講師 (50170022)

Project Period (FY) 2002 – 2003
KeywordsOvarian cancer / H-type sugar chain gene / sugar chain gene / α1,2-fucosyltransferase / glycolipids / phenotypically transformed cells / cell properties / anticancer drug resistance
Research Abstract

With the aim of elucidating the cytobiological significance of H genes in ovarian cancer cells, in this study we investigated changes in cell properties when H genes were transfected into cells. We inserted the human H-type sugar chain gene or a mouse α1,2-fucosyltransferase gene (MFUT-1,MFUT-2) into a mammalian expression vector, and then transfected the expression vector into cultures of human ovarian cancer cell line RMG-II. The H-type sugar chain gene encodes α1,2-fucosyltransferase, and its function is to add fucose. α1,2-Fucosyltransferase activity increased in the phenotypically transformed cells into which the human H gene and MFUT-1, MFUT-2 genes had been transfected, and the thin-layer chromatography immunostaining method showed that the level of expression of the Lewis Y-type sugar chain was higher than in the control RMG-II cell cultures. Expression of sialyl Lewis X-type sugar chains and sialyl-Lc4Cer was also observed in the control cells, but no expression of these sialylated sugar chains was observed in the phenotypic transformants. Assessment of cell properties showed that the phenotypically transformed cells into which the human H gene had been transfected were smaller than the control cells and that adhesion between the cells was stronger. Anticancer drug sensitivity testing showed that the phenotypically transformed cells were less sensitive to 5-FU than the control cells, and that the number of resistant cells was greater. These findings show that when an H gene is transfected into a human ovarian cancer cell line and α1,2-fucosyltransferase activity is increased, fucosylation of the cell membrane sugar chains increases, and sialylation decreases, and that these changes are accompanied by an increase in the strength of intercellular adhesion and a decrease in sensitivity to 5-FU. These findings suggest that fucosylated sugar chains contribute to intercellular adhesion strength and sensitivity to 5-FU in ovarian cancer cells.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Yuichi Sakuma, Kaneyuki Kubusiro, et al.: "Involvement of H type 1 carbohydrate antigen in cell adhesion to vascular endothelial cells of human endometrial cancer."Anticancer Research. 23. 1277-1282 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nobuo Masumoto, Kaneyuki Kubusiro, et al.: "Papanicolaou tests and molecular analyses using new fluid-based specimen collection technology in 3000 Japanese women."British Journal of Cancer. 88. 1883-1888 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroshi Yamashita, Kaneyuki Kubusiro, et al.: "Alteration in the metastatic potential of ovarian cancer cells by transfection of the antisense gene of β-1,4-galactosyltransferase."Oncol Reports. 10. 1857-1862 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitsuya Ishikawa, Kaneyuki Kubusiro, et al.: "Correlation of p16^<INK4a> overexpression with human papillomavirus infection in cervical adenocarcinomas."International Journal of Gynecological Pathology. 22. 378-385 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kyoko Tanaka, Kaneyuki Kubusiro, et al.: "Roles in the regulation of estrogen activity in human uterine endometrial carcinoma."Cancer Science. 94. 871-876 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yuichi Sakuma, Kaneyuki Kubushiro, et al.: "Involvement of H type 1 carbohydrate antigen in cell adhesion to vascular endothelial cells of human endometrial cancer."Anticancer Research. 23. 1277-1282 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nobuo Masumoto, Kaneyuki Kubushiro, et al.: "Papanicolaou tests and molecular analyses using new fluid-based specimen collection technology in 3000 Japanese women."British Journal of Cancer. 88. 1883-1888 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroshi Yamashita, Kaneyuki Kubushiro, et al.: "Alteration in the metastatic potential of ovarian cancer cells by transfection of the antisense gene of β-1,4-galactosyltransferase."Oncol Reports. 10. 1857-1862 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mitsuya Ishikawa, Kaneyuki Kubushiro, et al.: "Correlation of p16^<INK4a> overexpression with human papillomavirus infection in cervical adenocarcinomas."International Journal of Gynecological Pathology. 22. 378-385 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kyoko Takehara, Kaneyuki Kubushiro, et al.: "Estrogen sulfotransferase and sulfatase: Roles in the regulation of estrogen activity in human uterine endometrial carcinomas."Cancer Science. 94. 871-876 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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