2003 Fiscal Year Final Research Report Summary
Maternal Fatal cell traffic by DNA analysis and New approach for Autoinunune disorder
| Project/Area Number |
14571590
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | KANAZAWA MEDICAL UNIVERSITY |
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Principal Investigator |
YAMAGUCHI Nobuo KANAZAWA MEDICAL UNIVERSITY, Faculty of Medicine, professor, 医学部, 教授 (10106916)
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| Co-Investigator(Kenkyū-buntansha) |
NORIYOSHI Ogawa KANAZAWA MEDICAL UNIVERSITY, Faculty of Medicine, assistant professor, 医学部, 助教授 (80308618)
SUGIYAMA Kiyoshi Hoshi University, Faculty of Pharmacy, professor, 薬学部, 教授 (80145713)
IKAWA Hiromichi KANAZAWA MEDICAL UNIVERSITY, Faculty of Medicine, professor, 医学部, 教授 (20124935)
MATSUBA Shintaro KANAZAWA MEDICAL UNIVERSITY, Faculty of Medicine, assistant, 医学部, 助手 (40367462)
SHIMIZU Shoji KANAZAWA MEDICAL UNIVERSITY, Faculty of Medicine, assistant professor, 医学部, 助教授 (30150759)
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| Project Period (FY) |
2002 – 2003
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| Keywords | materno-fatal relation / pregnancy / MHC restriction / CD4 T cell / MHC / auto immunity / cell traffic / GVH |
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| Research Abstract |
We have previously reported that the immunization of pregnant mice with T-dependent antigens successfully induced suppression of the antigen-specific plaque-forming cell (PFC) response to the relevant antigens in the offspring. This suppression was not caused by the administered antigens, the antibodies produced by the pregnant mother or lactational transfer, but was dependent on the presence of the intact maternal T cells. It was major histocompatibility complex (MHC)-restricted manner tolerance, which continued for at least one-sixth of the marine life. Traditionally, the placenta acts as a natural barrier, not allowing the cells to pass through. However, the results presented strongly suggested that maternal T cells pass through the placenta and subsequently induce tolerance. In this present study, we attempted to substantiate the presence of maternal cells in the fetal circulation through the use of molecular techniques. We found that a highly polymorphic microsatellite sequence within the class II Eb gene of the H-2 complex is useful for the molecular detection of various H-2 alleles. DNA polymorphic analysis was used for tracking maternal H-2 alleles in the spleens of baby mice.
According to our fundamental approaches, next we tried to prove the maternal cell trafficking in human system, after satisfactory organizing informed consents. We tried to picked up the HLA locus A, B, C, DR, DQ of mother and her children, aged 11 and 13 years old. But we have not yet obtained complete trafficking of mother cell that showed whole set of HLA locus from mother in her children. We have to increase the case and select the patient of autoimmune syndrome such as SLE etc.
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