2003 Fiscal Year Final Research Report Summary
Involvement of transcription factors in apoptosis of the Retinal Ganglion Cells by Optic Nerve Transection.
| Project/Area Number |
14571651
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
YOSHIDA Kazuhiko Hokkaido Univ.Hospital, Inst., 医学部・歯学部附属病院, 助手 (90281807)
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| Project Period (FY) |
2002 – 2003
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| Keywords | c-Jun / phosphorylation / retina / optic nerve / apoptosis / knock-in mouse / TUNEL staining / immunocytochemistry |
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| Research Abstract |
To examine the involvement of c-Jun and c-Jun N-terminal phosphorylation (JNP) in apoptosis of retinal ganglion cells (RGCs) after the optic nerve (ON) transection, the expression and phosphorylation of c-Jun protein and apoptosis in RGCs were examined after ON transection in wild-type mice and mice in which both phosphoacceptor serines of Jun have mutated to alanines (c-Jun[AA] mice). The fluorescent tracer 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) was applied to the superior colliculi (SC), and the right ON was severed after 7 days. After two more weeks, the average number of RGCs per field was calculated. JNP and TUNEL-labeled apoptotic nuclei were detected in the ganglion cell layer (GCL) of the retina of the wild-type mice in response to ON transection. The numbers of TUNEL-positive nuclei in the c-Jun(AA) mice was reduced in comparison to those in wild-type mice. Retrograde labeling showed that the number of the RGCs in the retinas on the injured side of the c-Jun(AA) mice was significantly higher than in wild-type mice 14 days after the lesion. These results suggest that there is a partial but significant contribution of JNP to the induction of apoptosis m RGCs by ON transaction.
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Research Products
(22 results)
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[Publications] Kazuhiko Yoshida, Satoru Kase, Keiko Nakayama, Hiroyasu Nagahama, Takayuki Harada, Hiromi Ikeda, Chikako Harada, Junko Imaki, Kazuhiro Ohgami, Kenji Shiratori, Iliyana Bozhidarova Ilieva, Shigeaki Ohno, Shinzo Nishi, Kei-Ichi Nakayama: "Involvement of p27(KIJP1) in the proliferation of the developing corneal endothelium."Investigative ophthalmology and visual science. (in press).
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yoshida K, Kase S, Nakayama K, Nagahama H, Harada T, Ikeda H, Harada C, Imaki J, Ohgami K, Shiratori K, Ohno S, Nakayama KI.: "Distribution of p27(KIP1), cyclin D1, and proliferating cell nuclear antigen after retinal detachment."Graefes Arch Clin Exp Ophthalmol.. (in press).
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yoshida K, Nakayama K, Kase S, Nagahama H, Harada T, Ikeda H, Harada C, Imaki J, Ohgami K, Shiratori K, Ohno S, Nishi S, Nakayama KI: "Involvement of p27(KIP1) in proliferation of the retinal pigment epithelium and ciliary body."Anal Embryol (Berl). (in press).
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[Publications] Yoshida K, Nakayama K, Nagahama H, Harada T, Harada C, Imaki J, Matsuda A, Yamamoto K, Ito M, Ohno S, Nakayama KI.: "Involvement of p27(KIP1) Degradation by Skp2 in the Regulation of Proliferation in Response to Wounding of Cornea] Epithelium."Invest Ophthalmol Vis Sci.. 43. 364-370 (2002)
Description
「研究成果報告書概要(欧文)」より
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