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2003 Fiscal Year Final Research Report Summary

Immunocytochemical study of prorenin procrssing in the mouse submandibular gland, using antibodies against prorenin and its cleaved fragments.

Research Project

Project/Area Number 14571753
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionThe Nippon Dental University

Principal Investigator

KURABUCHI Shingo  The Nippon Dental University, School of Dentistry at Tokyo, Associate Professor, 歯学部, 助教授 (90170076)

Project Period (FY) 2002 – 2003
KeywordsProrenin / Renin / Processing / Immunohistochemistry / Immunoelectron microscopy / Granular convoluted tubulule / Submandibular gland / Mouse
Research Abstract

According to the complete amino acid sequence of the mouse submandibular gland renin precursor (Ren-2-prorenin) (Panthier et al.,1982; Misono et al.,1982), the short peptides, which stride the positions of proteic cleavages, each occuring after three basic residues : Cys 18-Thr 19, Mg 63-Der 64, and Mg 363-Asp 364, respectively, were designed, chemically synthesized, and immunized rabbits. The preparation of specific antibody against the second peptide, ST-161: Thr-Lys-Arg-Ser-Ser-Leu-Thr-Asp-Leu-Ile-Cys, which strides between the Lys62-Arg63 was succeeded. This antibody seemes to recognize prorenin, but not mature renin. Western blotting analysis, however, was not performed to examin the specificity of the antibody. It was unluckly that other peptides designed by the author were not reacted as the antigen, so, the antibodies were not obtained.
The immunocytochemical localization and distribution of the ST-161 was examined in the mouse submandibular gland by indirect Cy3 (indocarbocyanine)-labeled antibody method for light microscopy. Red immunoreactive materrials were localized at the pen-nuclear region of the granular convoluted tubule cells, but not on the secretory granules. These immunostain-ed region was corresponding to the trans-Golgi network, containing Golgi apparatus and immature secretory granules. It is therefore concluded that the corresponding portion of mouse Ren-2-prorenin may be cleaved off in the Golgi apparatus or immumature secretory granules. It has not been successful to date to confirm this idea by immunoelectron microscopy.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Kurabuchi S, Hosoi K, Gresik EW: "Developmental and androgenic regulation of the immunocytochemical distribution of mK1, a true tissue kallikrein, in the granular convoluted tubule of the mouse submandibular gland."The Journal Histochemistry & Cytochemistry. 50. 135-145 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kurabuchi S, Tanaka S: "Immunocytochemical localization of prohormone convertases PC1 and PC2 in the mouse thyroid gland and repiratory tract."The Journal Histochemistry & Cytochemistry. 50. 903-909 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kurabuchi S, Hosoi K, Gresik EW: "Multihormonal regulation of granular convoluted tubule cells expressing mKl, atrue tissue kallikrein, in the mouse submandibular gland."Proceedings of the 21st Conference of European Comparative Endocrinologists. Monduzzi Editore, MEDIMOND INC.. 527-530 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kurabuchi S: "Morphogenic changes in the granular convoluted tubule cells of the mouse submandibular gland following hypophysectomy and hormonal replacement."Odontology. 90. 27-34 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kurabuchi S: "Thyroid hormone regulation of granular intercalated duct cells in the submandibular glands of female mice."Odontology. 91. 7-12 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yaoi Y, Suzuki M, Tomura H, Kurabuchi S, Sasayama Y, Tanaka S: "Expression and localization of prohormone convertase PC1 in the calcitonin-producing cells of the bullfrog ultimobranchial gland."The Journal Histochemistry & Cytochemistry. 51. 1459-1466 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kurabuchi S, Hosoi K, Gresik EW: "Developmental and androgenic regulation of the irnmunocytochemical distribution of mK1, a true tissue kallikrein, in the granular convoluted tubule of the mouse"The Journal Histochemistry & Cytochemistry. 50. 135-145 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurabuchi S, Tanaka S: "Immunocytochemical localization of prohormone convertases PC1 and PC2 in the mouse thyroid gland and repiratory tract."The Journal Histochemistry & Cytochemistry. 50. 903-909 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurabuchi S, Hosoi K, Gresik EW: "Multihormonal regulation of granular convoluted tubule cells expressing mK1, atrue tissue kallikrein, in the mouse submandibular gland."Proceedings of the 21st Conference of European Comparative Endocrinologists (Monduzzi Editore)(MEDJIMOND INC.). 527-530 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurabuchi S: "Morphogenic changes in the granular convoluted tubule cells of the mouse submandibular gland following hypophysectomy and hormonal replacement."Odontology. 90. 27-34 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurabuchi S: "Thyroid hormone regulation of granular intercalated duct cells in the submandibular glands of female mice."Odonyology. 91. 7-12 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yaoi Y, Suzuki M, Tomura H, Kurabuchi S, Sasayama Y, Tanaka S: "Expression and localization of prohormone convertase PC1 in the calcitonin-producing cells of the bullfrog ultimobranchial gland."The Journal Histochemistry & Cytochemistry. 51. 1459-1466 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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