2003 Fiscal Year Final Research Report Summary
Biomimetic surface modification of titanium implants
| Project/Area Number |
14571847
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
ABE Yasuhiko Hiroshima University, Medical and Dental Hospital, Assistant Professor, 医学部・歯学部附属病院, 講師 (00253097)
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| Co-Investigator(Kenkyū-buntansha) |
YAMANAKA Takenori Hiroshima University, Graduate School of Biomedical Sciences, Research Associate, 大学院・医歯薬学総合研究科, 助手 (20325202)
OKAZAKI Masayuki Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (30107073)
AKAGAWA Yasumasa Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (00127599)
HIASA Kyou Hiroshima University, Graduate School of Biomedical Sciences, Research Associate, 大学院・医歯薬学総合研究科, 助手 (60304432)
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| Project Period (FY) |
2002 – 2003
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| Keywords | Implant / Titanium / Acids / Surface modification / Phospho-amino acid / Osteoblasts / Cell attachment |
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| Research Abstract |
Purpose : The purpose of this stud was to develop a new biomimetic surface modification of titanium implat with phospho-amino acid and evaluate its effectiveness on initial attachment of osteoblasts MC3T3-E1. Method Commercially pure titanium (cp Ti disks diameter : 5.8 mm thickness : 2 mm ; machined surface Ra : 0.15 micron JIS 2 ; GC Co, Tokyo, Japan) were ultrasonically treated with 10 N hydrochloric acid (HCl) and ultrapure water at room temperature for 30 min respectively. Then these disks were modified with O-phospho-L-threonine (P-Thr) at 37 C degree far 12 hrs followed ultrasonic rinsing with ultrapure water for 10 min. Untreated disks were used as a control. X-ray hotoelectron spectroscopy (XPS) chemically analyzed the P-Thr-modified disk surfaces in comparison with the control. In addition initial attachment of osteoblasts MC3T3-E1 to each of modifed surfaces and the control was evaluated by the percentage of attached cells at 10 30 60 min respectively. Results : XPS revealed that P-Thr chemically bonded to the surface treated with HCl and ultrapure water since the significant chemical shift (t-test : P < 0.05) of P 2p binding energy of P-Thr was detected. Percentage at 10 min of cellular attachment to the P-Thr-modified surface was significantly higher than that of the control (one-way ANOV and Bonferroni/Dunn's test : P < 0,01). Conclusion : It is concluded that chemical banding of P-Thr to the cp Ti surface treated with HCl and ultrapure water was detected and the P-Thr-modified surface can accelerate the initial osteoblasts attachment
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