2003 Fiscal Year Final Research Report Summary
Isolation of cell lines with high potentials for lymphatic metastasis from active Rho-Kinase-transfected oral carcinoma and clarification of their machanism of metastasis
| Project/Area Number |
14571888
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kobe University |
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Principal Investigator |
KOMORI Takahide Kobe University, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (50251294)
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| Co-Investigator(Kenkyū-buntansha) |
AMANO Mutsuki Nagoya University, Graduate School of Medicine, Assistant Professor, 大学院・医学研究科, 講師 (90304170)
FUJIOKA Manabu Kobe University Hospital, Instructor, 医学部附属病院, 助手 (70362788)
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| Project Period (FY) |
2002 – 2003
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| Keywords | ORAL CANCER / METASTASIS / Rho-kinase |
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| Research Abstract |
Rho-kinase, an effector molecule of Rho gene, may reduce cellular adhesive potential (indispensable for cancer invasion and matastasis) and stimulate cancer's migrating potentials. The functions and actionmechanisms of Rho-kinase have been attracting close attention. Wepreviously reported that Rho-kinase and myosin phosphatase are involved in the regulation of microtubules as well as actomyosin thread regulation.
To date, hardly any studies have been published concerning metastasis of human oral carcinoma cells. In our previous studies, of oncogenes is in nude mice with oral carcinoma cells implanted on their backs, cancer cell matastasis to other organs (including lymph nodes) was not observed.
To study metastasis of oral carcinoma, it is necessary to develop a model of metastasis allowing a comparison between cancer cell lines with metastasizing potentials and those having oncogenic potentials but no metastasizing potentials. The present study was undertaken to obtain stable transfecta
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nts permanently expressing active type Rho-kinase. A plasmid inducing high Rho-kinase expression was introduced to primary squamous cell carcinoma cell line NOS-1 of mandibular gingiva to examine the possibility of isolating tumor cell lines with high metastatic potentials by reinforcing malignancy level and migration potentials.
We obtained stable transfectants permanently expressing active type Rho-kinase. When measured by the gold colloid method. the migration potentials of the clones of these transfectants was about twice as great as that of the control cells. This indicates enhanced migration potentials of the clones expressing active type Rho-kinase. In orthotopic implantation of these transfectants to the nude mouse tongue, tumor take and oncogenic potentials were noted. but no matastasis to lymph nodes or other organs was observed.
In this experiment. migration potentials of the clones expressing active type Rho-kinase were enhanced, but the potential of metaslasizing to other organs Less
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