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2003 Fiscal Year Final Research Report Summary

Experimental Studies on the effects of BMP-2 Expressing Adenoviral Vector to the Distraction Osteogenesis

Research Project

Project/Area Number 14571924
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionKANAZAWA MEDICAL UNIVERSITY

Principal Investigator

FUJIMURA Kazuma  Kanazawa Medical University, Department of Oral and Maxillofacial Surgery, Associate Professor, 医学部, 助教授 (30252399)

Co-Investigator(Kenkyū-buntansha) SEGAMI Natsuki  Kanazawa Medical University, Department of Oral and Maxillofacial Surgery, Professor, 医学部, 教授 (40148721)
KANEYAMA Keiseki  Kanazawa Medical University, Department of Oral and Maxillofacial Surgery, Assistant Professor, 医学部, 助手 (50329380)
Project Period (FY) 2002 – 2003
KeywordsAdenoviral vector / Distraction / Osteogenesis / Bone Morphogenetic Protein
Research Abstract

1.To examine the effectiveness of a gene transfer procedure for the delivery of BMP-2 to the distraction osteogenesis, we constructed a human BMP-2--expressing replication-deficient adenoviral vector, AxCAOBMP-2, and evaluated its osteoinductive activity in vitro and in vivo.
2.C2C12 myoblasts were infected in vitro with this viral vector or an Escherichia coil LacZ gene-expressing control adenovirus vector (AxCALacZ). Twenty-four hours after the infection, indirect immunofluorescence was performed. On day 5 after the infection, alkaline phosphatase (ALP) in the cells and osteocalcin in the culture medium were measured. Furthermore, to examine the effectiveness of gene transfer of BMP-2 in vivo, we evaluated osteoinduction by AxCAOBMP-2, under transient immunosuppression with cyclophosphamide, given at a dose of 125 mg/kg intraperitoneally the day before injection of the adenoviral vector. Twenty-five microliters of AxCAOBMP-2 (8.75 x 10^8 plaque-forming units [pfu],'Group I) and AxCALa … More cZ (1.75 x 10^8 pfu, control group) and 5 microl of AxCAOBMP-2 (1.75x 10^8 pfu, Group II) were injected into a right calf muscle of Wistar rats. On day 21, bone formation in each group was investigated radiologically and histologically.
3.Abundant BMP-2 expression in C2C12 cells infected with this viral vector was confirmed by immunofluorescence. C2C12 cellstransferred with the BMP-2 gene by this vector produced ALP in the cells and also produced and secreted osteocalcin in the culture medium. Osteoinduction was found only in the AxCAOBMP-2 treated groups with immunosuppression. Osteoinduction activity was higher in Group I than in Group II.
3.Twelve animals, performed distraction, were treated with AxCAOBMP-2 (8.75 x 10^8 pfu) 25μg in each, during distraction osteogenesis at 7 days and 14 days. In results, Bone formation and ALP activity were increased significantly in comparison with non-treatment groups. It was suggested that Gene transfer procedure for the delivery of BMP-2 effected to the distraction osteogenesis and had a potential shorting distraction period. Less

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Fujimura K, Bessho K, et al.: "The effect of fibroblast growth factor-2 to osteoinducing activity by recombinant human bone morphogenetic protein-2 in rat muscle."Arch Oral Biol.. 48. 577-584 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] (Y.Okubo), K.Fujimura, et al.: "Expression of bone morphogenetic protein-2 in the course of osteoinduction by recombinant human bone morphogenetic protein-2."Clin Oral Impl Res.. 13. 80-85 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] (Kusumoto K), Fujimura K, et al.: "Osteoinduction by recombinant human bone morphogenetic protein-2 in muscles of non-human primates."J Int Med Res.. 30. 251-259 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] (Suzuki T), Fujimura K, et al.: "Regeneration of defects in the articular cartilage in rabbit temporomandibular joints by bone morphogenetic protein-2."Br J Oral Maxillofac Surg. 40. 201-206 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] (Y.Okubo), K.Fujimura, et al.: "The preclinical study of recombinant human bone morphogenetic protein-2: Application of hyperbaric oxygenation during bone formation in low blood supply tissue."Int J Oral Maxillofac Surg.. 32. 313-317 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujimura K, Bessho K, Okubo Y, et al.: "A bioactive bone cement containing Bis-GMA resin and A-W Glass-ceramic as an augmentation graft material on mandibular bone."Clin Oral Impl Res.. 14. 659-667 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujimura K, Bessho K, Okubo Y, Kusumoto K, Segami N, Iizuka. T: "The effect of fibroblast growth factor-2 to osteoinducing activity by recombinant human bone morphogenetic protein-2 in rat muscle."Arch Oral Biol.. 47(8). 577-584 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Okubo, K.Bessho, K.Fujimura, K.Kusumoto, Y.Ogawa.T.Iizuka.: "Expression of bone morphogenetic protein-2 in the course of osteoinduction by recombinant human bone morphogenetic protein-2."Clin Oral Impl Res.. 13. 80-85 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kusumoto K, Bessho K, Fujimura K, Akioka J, Okubo Y, Wang Y, Iizuka T, Ogawa Y.: "Osteoinduction by recombinant human bone morphogenetic protein-2 in muscles of non-human primates."J Int Med Res.. 30(3). 251-259 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki T, Bessho K, Fujimura K, Okubo Y, Segami N, Iizuka T.: "Regeneration of defects in the articular cartilage in rabbit temporomandibular joints by bone morphogenetic protein-2."Br J Oral Maxillofac Surg. 40(3). 201-206 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Okubo, K.Bessho, K.Fujimura, Y.Kusumoto, T.Suzuki, N.Segami.Y.Ogawa, T.Iizuka.: "The preclinical study of recombinant human bone morphogenetic protein-2 : Application of hyperbaric oxygenation during bone formation in low blood supply tissue."Int J Oral Maxillofac Surg.. 32(3). 313-317 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujimura K, Bessho K, Okubo Y, Segami N, Iizuka. T.: "A bioactive bone cement containing Bis-GMA resin and A-W Glass-ceramic as an augmentation graft material on mandibular bone."Clin Oral Impl Res.. 14(5). 659-667 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keiseki Kaneyama, Natsuki Segami, Masaaki Nishimura, Jun Sato, Toshikazu Suzuki, Kazuma Fujimura.: "Osteoclastogenesis inhibitory factor/osteoprotegerin in synovial fluid from patients with temporomandibular disorders."Int J Oral Maxillofac Surg.. 32(4). 404-407 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] 海原真治, 別所和久, 藤村和磨, 大久保康則, 園部純也, 楠本健司, 飯塚忠彦: "アテロコラーゲンスポンジ(TERUPLUG【○!R】)を担体とする遺伝子組み換えヒト骨形成因子の骨誘導に関する実験的研究-アテロペプチドコラーゲン(Cellmatrix LA【○!R】)を担体とした場合との比較-"日口外誌. 49(4). 241-245 (2003)

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      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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