A study of the Calcification by matrix vesicles in closure of immature teeth

2003 Fiscal Year Final Research Report Summary

• Project/Area Number: 14571961
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 矯正・小児・社会系歯学
• Research Institution: Kyushu Dental College
• Principal Investigator: MAKI Kenshi Kyushu Dental College, Pediatric Dentistry, Associate Professor, 歯学部, 助教授 (60209400)
• Co-Investigator(Kenkyū-buntansha): KIMURA Mitsutaka Kyushu Dental College, Pediatric Dentistry, Professor, 歯学部, 教授 (70047801) ; NOGUCHI Jiroo Kyushu Dental College, Biochemistry, Professor, 歯学部, 教授 (30073688) ; KIMURA Mitsutaka Kyushu Dental College, Pediatric Dentistry, Professor (70047801) ; NOGUCHI Jiroo Kyushu Dental College, Pediatric Dentistry, Professor (30073688) ; KIMURA Mitsutaka Kyushu Dental College, Pediatric Dentistry, Professor (70047801)
• Project Period (FY): 2002 – 2003
• Keywords: Odontollast / Matrix Vesicle / Protease / Lactate Dehydrogenate

Research Abstract

Matrix vesicles are extracellular organelles that initiate mineralization in the extracellular that initiate mineralization in the extracellular matrix of carious calcifying tissues. The matrix vesicles fractions from the dentin of fetal bovine, obtained by collagenase digestion and differential centrifugation, were subjected to sucrose-density-gradient centrifugation.
In this report, we investigated the enzymes alkaline phosphates activity( ALP), protease activity lactate dehydrogenase(LDH) and protease to evaluate the matrix vesicles. We found not only matrix vesicles but also matrix vesicle like vesicles without protease in the dentin of fetal bovine. Protease activity after inhibition with o-phenanthroline was restored by the addition of Co<2+>, A1<2+>, Zn<2+>, Mg<2+>,. These results show that the protease in the matrix vesicles in the dentin is a metalloprotease.