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2003 Fiscal Year Final Research Report Summary

A study of the Calcification by matrix vesicles in closure of immature teeth

Research Project

Project/Area Number 14571961
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 矯正・小児・社会系歯学
Research InstitutionKyushu Dental College

Principal Investigator

MAKI Kenshi  Kyushu Dental College, Pediatric Dentistry, Associate Professor, 歯学部, 助教授 (60209400)

Co-Investigator(Kenkyū-buntansha) KIMURA Mitsutaka  Kyushu Dental College, Pediatric Dentistry, Professor, 歯学部, 教授 (70047801)
NOGUCHI Jiroo  Kyushu Dental College, Biochemistry, Professor, 歯学部, 教授 (30073688)
KIMURA Mitsutaka  Kyushu Dental College, Pediatric Dentistry, Professor (70047801)
NOGUCHI Jiroo  Kyushu Dental College, Pediatric Dentistry, Professor (30073688)
KIMURA Mitsutaka  Kyushu Dental College, Pediatric Dentistry, Professor (70047801)
Project Period (FY) 2002 – 2003
KeywordsOdontollast / Matrix Vesicle / Protease / Lactate Dehydrogenate
Research Abstract

Matrix vesicles are extracellular organelles that initiate mineralization in the extracellular that initiate mineralization in the extracellular matrix of carious calcifying tissues. The matrix vesicles fractions from the dentin of fetal bovine, obtained by collagenase digestion and differential centrifugation, were subjected to sucrose-density-gradient centrifugation.
In this report, we investigated the enzymes alkaline phosphates activity( ALP), protease activity lactate dehydrogenase(LDH) and protease to evaluate the matrix vesicles. We found not only matrix vesicles but also matrix vesicle like vesicles without protease in the dentin of fetal bovine. Protease activity after inhibition with o-phenanthroline was restored by the addition of Co<2+>, A1<2+>, Zn<2+>, Mg<2+>,. These results show that the protease in the matrix vesicles in the dentin is a metalloprotease.

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Published: 2005-04-19  

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