2004 Fiscal Year Final Research Report Summary
Development of Columns for Normal-Phase HPLC Using Silica Gels immobilized Metal-Phthalocyanine derivatives
| Project/Area Number |
14572031
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Okayama University |
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Principal Investigator |
MIFUNE Masaki Okayama Univ., Fac.of Pharm.Sci., Associate Prof., 薬学部, 助教授 (00200105)
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| Project Period (FY) |
2002 – 2004
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| Keywords | Silica gels immobilized metal-phthalocyanine derivatives / normal phase HPLC / polyaromatic hydrocarbons / interactions concerning π-electrons / π-d interaction / steroid compounds |
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| Research Abstract |
In order to develop columns packed silica gels immobilized metal-phthalocyanine derivatives (M-PCS_D columns (M=Cu^<2+> or Ni^<2+>)) for normal phase HPLC, the retention behaviors of 1-4 ring polyaromatic hydrocarbons(PAH) that have no functional groups in non-polar mobile phase were examined first, PAHs could be separated in the order of ring numbers by the use M-PCS_D Columns. Therefore, the interaction between PAHs and M-PCS_D were studied by various examinations, such of immobilized amount of M-PCS on the silica gel, the comparison of retention behaviors of PAH in the polar and non-polar mobile phase or influence of retention behaviors of PAHs in non-polar mobile phase containing compounds that have π-electron. In the result, it was revealed that the interactions concerning π-electrons between PAHs and M-PCS on M-PCS_D were main in the separation of PAHs in non-polar mobile phase. The retention behaviors of PAHs were influenced by central metals of M-PCS. It was suggested that Ni-PCS_D has the π-d interaction in addition of the interactions concerning π-electrons. The analytical applications normal phase HPLC using M-PCS_D columns were examined using steroid compounds or 17 PAHs that were regulated by US Environmental Protection Agency (EPA). It was revealed that the steroid compounds could be separated in n-hexane and chloroform mobile phase and 17 PAHs were also separated by the use of gradient system using n-hexane and chloroform. Thus, M-PCS_D columns developed for normal phase HPLC.
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