• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2003 Fiscal Year Final Research Report Summary

Development of Separation Methods for Biological Macromolecules and Cells Using Nonsynchronous Coil Planet Centrifuge

Research Project

Project/Area Number 14572039
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionNihon University

Principal Investigator

SHINOMIYA Kazufusa  Nihon University, College of Pharmacy, Associate professor, 薬学部, 助教授 (70215995)

Project Period (FY) 2002 – 2003
Keywordscountercurrent chromatoeraphy / aqueous two-phase partitioning / protein separation / cell separation / chromatosgraphy / separation analysis / biological macromolecule / blood cell
Research Abstract

1. Protein separation using the nonsynchronous coil planet centrifuge (CPC)
The nonsynchronous CPC was employed to investigate the optimal separating conditions and the effect of planetary motion of coiled column on the partition efficiency using the protein separation with aqueous-aqueous polymer phase systems. The separation was examined using three kinds coiled column with each different volume (11, 24 and 39 mL), a two-phase system composed of 12.5% (w/w) polyethylene glycol 1000 and 12.5% (w/w) dibasic potassium phosphate and a set of stable proteins for samples including cytochrome C, myoglobin and lysozyme. The best separation was obtained using the 39 mL multilayer coil at the head to tail elution mode with the revolution at 800 rpm and the rotation at 10 rpm, where the similar result was obtained in the eccentric coil under same experimental conditions. The further experiments also revealed that the best result was obtained in the head to tail elution mode by the clockwise (CW) coil rotation for the lower mobile phase or by the counterclockwise (CCW) coil rotation for the upper mobile phase, both under CCW revolution of the rotor.
2. Elutriation of blood cell components by the nonsynchronous CPC
The nonsynchronous CPC was applied to the separation of blood cell components using a physiological buffer solution. The optimal condition for the separation of sheep blood samples was performed at the CCW revolution and the CW rotation of coiled column by the head to tail elution mode. Under the high speed of revolution of 600-1000 rpm, sheep and human blood samples were each completely separated and eluted into two peaks, where all cell components other than erythrocytes such as platelets and leukocytes were eluted at 0 rpm and erytbrocytes at 10 rpm of coil rotation. The normal elution pattern was achieved at 800 rpm of revolution for sheep blood and at 700 rpm for human blood samples.

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] Kazufusa Shinomiya et al.: "Protein separation by nonsynchronous coil planet centrifuge with aqueous-aqueous polymer phase systems"Journal of Chromatography A. 1005. 103-112 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazufusa Shinomiya, Yozo Kabasawa, Kazuhiro Yanagidaira, Haruo Sasaki, Minoru Muto, Tadashi Okada, Yoichiro Ito: "Protein separation by nonsynchronous coil planet centrifuge with aqueous-aqueous polymer phase systems"Journal of Chromatography A. 1005. 103-112 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2005-04-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi