2003 Fiscal Year Final Research Report Summary
Study on the System Detecting the residual Leukeinic Cells after the Gene-targeted Therapy forLeukemia
| Project/Area Number |
14572176
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
MARA Nobuo Tokyo Medical and Dental University, Center for Education Research in Medicine and Dentistry, Professor, 医歯学教育システム研究センター, 教授 (00142258)
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| Co-Investigator(Kenkyū-buntansha) |
MURAKAMI Naomi Tokyo Medical and Dental University, Department of Medicine, Assistant, 医学部附属病院, 助手 (50143582)
TONOA Shinji Tokyo Medical and Dental University, Postgraduate School, Associate Professor, 大学院・医歯学総合研究所, 助教授 (80251510)
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| Project Period (FY) |
2002 – 2003
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| Keywords | Acute Myelovlastiv Leukemia / Residnal Leukemia Cells / Gene-targeted Therapy |
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| Research Abstract |
1.Leukeinic Cell Culture.
Leukemic blast progenitors were cultured either in methylcellulose media and suspension culture before and after the leukemic patients recieved therapy. The growth of blast progenitors in methylcellulose showed their proliferative activity, while the growth in suspension supported their self-renewal capacity.
2.Detection of Chimeric Fusion Oncogenes
Some leukemic cells showed typical chimeric fusion oncogene such as PML/RARA, AML1/MTG8J and BCR/ABL. Detection of these genes showed the presence of leukemic cells even if leukemia patients were clinically considered as in remission. Firtherinore we studied the effects of Notch ligand on the grwoth of U937 leukemic cell line in the presence of GM-CSF.
3.Resuits
There was a significant correlation between the residual leukemic cells with high self-renewal capacity and/or chimeric fusion oncogene and the clinical course of the patients with acute leukemia. Those patients whose leukemic blast progenitors had high self-renewal capcity showed much poor prognosis.
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