2003 Fiscal Year Final Research Report Summary
Proteome analysis of human macrophage cells associated with atherosclerosis
| Project/Area Number |
14572179
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Gifu University |
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Principal Investigator |
FUJII Hidehiko Gifu University, School of Medicine, Research Associate, 医学部, 助手 (50301213)
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| Co-Investigator(Kenkyū-buntansha) |
SEISHIMA Mitsuru Gifu University, School of Medicine, Professor, 医学部, 教授 (10171315)
WADA Hisayasu Gifu University, Hospital, Assistant Professor, 医学部附属病院, 講師 (10283300)
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| Project Period (FY) |
2002 – 2003
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| Keywords | Proteomics / Macrophage / Atherosclerosis |
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| Research Abstract |
Lipopolysaccharide (LPS) is one of the potent activators of innate immune response and elicits the activation of an intracellular cascade via Toll like receptors. This signaling pathway leads to the activation of a transcription factor NF-kB, which initiates the transcription of proinflammatory cytokine genes. Recent data including cDNA microarray analysis, revealed that LPS activated pathway is more complicated.
A proteomic approach, using high-sensitivity mass spectrometry, particularly nano-electrospray mass spectrometry (ESI-MS/MS), provides a complementary and potentially comprehensive approach to the analysis of signaling mechanisms by solving the expressed proteins of the cell followed by protein sequencing and identification. Proteomic approach may lead more sophisticated in vitro assays for LPS activated pathway.
The aim of this study is to investigate the changes in human monocyte/macrophage protein expression stimulated by LPS exposure and to understand the comprehensive activated macrophage proteome. In addition to a 2-dimensional gel elecrophoresis approach, we applied an HPLC-based proteomics approach, including 2 dimensional liquid chromatography (2DLC) system. We have identified 236 proteins from human monocyte/macrophage cells. 25 LPS induced proteins including signaling, apoptosis and proliferation proteins were identified. Several proteins that had not been known in the LPS-induced pathway were found. Our data provides reference for the LPS activated macrophage proteome.
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