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2003 Fiscal Year Final Research Report Summary

Effect of acute exercise on activation of the calcineurin signaling pathway involved in development of slow muscle fibers

Research Project

Project/Area Number 14580055
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 体育学
Research InstitutionKyorin University

Principal Investigator

HITOMI Yoshiaki  Kyorin University, School of Medicine, Research Associate, 医学部, 助手 (70231545)

Co-Investigator(Kenkyū-buntansha) OHNO Hideki  Kyorin University, School of Medicine, Professor, 医学部, 教授 (00133819)
Project Period (FY) 2002 – 2003
KeywordsSkeletal muscle / Exercise / Slow muscle fiber / Fast muscle fiber / Calcineurin / MCIP1 / NFAT / Transcription
Research Abstract

To investigate the role of the calcineurin in development of slow muscle fibers, we first compared the mRNA expression of genes in the calcineurin signaling pathway in slow muscle fibers with that in fast muscle fibers. It revealed that the mRNA expression of myocyte specific calcineurin inhibitory protein 1 (MCIP1) was at least 10 times higher in slow muscle fibers than in fast muscle fibers. The difference in the MCIP1 mRNA expression was lost, when slow and fast muscle fibers lost their differentiated phenotypes after denervation of skeletal muscles.
The expression of MCIP1 mRNA was transiently induced in skeletal muscles within 4 hours after acute exercise. In the MCIP1 promoter sequence, there is an array of tentative NFAT binding sites, which might respond to the calcineurin activity. Indeed, calcineurin directly regulated the MCIP1 promoter via these NFAT binding sites. Therefore, the expression levels of MCIP1 mRNA might directly reflect the calcineurin activity in skeletal muscle, which is otherwise very difficult to monitor in vivo.
We next examined the relationship between the intensity of acute exercise and the induction of MCIP1 mRNA in rat skeletal muscles, after treadmill running at various speed and time. The expression of MCIP1 mRNA was proportionally correlated with the intensity of acute exercise, and it was induced by very mild exercise (12 m/min for 10 min). Even when varying speed from 8〜16 m/min for 120 m, the MCIP1 mRNA was equally induced in rat skeletal muscles. These results suggest that the expression level of MCIP1 mRNA is a good indicator of the calcineurin activity in skeletal muscle, and even very mild exercise is enough to activate the calcineurin signaling pathway, which is important for development and maintenance of slow muscle fibers.

  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Ohno H: "Extracellular superoxide dismutase and lifestyle related diseases (Review)"Curr Top Pharmacol. 1. 1-15 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohno H: "Hematological responses in juveniles after training at moderate altitude"Adv Exerc Sports Physiol. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohno H: "We have two strategies to attain healthy ageing"Geriatr Gerontol Int. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takemasa T: "Simple method for the identification of oxidative fibers in skeletal muscle"Eur J Appl Physiol. 91. 357-359 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hitomi Y: "Effect of Acute Exercise on Expression of mRNA Involved in the Calcineurin Signaling Pathway in Human Skeletal Muscle"IUBMB Life. 55. 409-413 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hitomi Y: "Intermittent hypobaric hypoxia increases the ability of neutrophils to generate superoxide anion in humans"Clin Exp Pharmacol Physiol. 30. 659-664 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohno H: "Physical exercise and extracellular superoxide dismutase : Antiarthrogenic effects"Adv Exerc Sports Physiol. 9. 45-53 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ueda H: "Enhanced expression of neuronal nitric oxide synthase in islets of exercise-trained rats"Biochem Biophys Res Commun. 19. 794-800 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki K: "Genetic variation in hypoxia-inducible factor lalpha and its possible association with high altitude adaptation in Sherpas"Med Hypotheses. 61. 385-389 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimoto K: "Gliclazide protects pancreatic beta-cells from damage by hydrogen peroxide"Biochem Biophys Res Commun. 303. 112-119 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyazaki H: "Effect of eccentric exercise on neutrophil function in human plasma"Adv Exerc Sports Physiol. 9. 69-75 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kizaki T: "Uncoupling protein 2 plays an important role in nitric oxide production of lipopolysaccharide-stimulated macrophages"Proc Natl Acad Sci USA. 99. 9392-9397 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Muto M: "Association between aldehyde dehydrogenase gene polymorphisms and the phenomenon of field cancerization in patients with head and neck cancer"Carcinogenesis. 23. 1759-1765 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato K: "Hyperstable U1snRNA complementary to the K-ras transcripts induces cell death in pancreatic cancer cells"Br J Cancer. 87. 898-904 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ookawara T: "Transcription regulation of gene expression in human skeletal muscle in response to endurance training"Res Commun Mol Pathol Pharmacol. 111. 41-54 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki K: "Association of SH2-B to phosphorylated tyrosine residues in the activation loop of TrkB"Res Commun Mol Pathol Pharmacol. 111. 27-39 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 人見嘉哲: "トレーニング生理学"杏林書院. 375 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohno H, Kizaki T, Suzuki K, Hitomi Y, Watanabe S, Ookawara T, Suzuki K, Haga S, Saitoh D, Radak Z, Ji LL.: "Extracellular superoxide dismutase and lifestyle related diseases (Review)."Curr Top Pharmacol. 1. 1-15 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohno H, Kim CK, Kim JH, Jung YS, Bae SY, Toshinai K, Miyazaki H, Esaki K, Takemasa T, Kinugasa T, Kizaki T, Hitomi Y, Ookawara T, Oh-ishi S, Haga S.: "Hematological responses in juveniles after training at moderate altitude."Adv Exerc Sports Physiol. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohno H, Kizaki T, Hitomi Y, Watanabe S, Ishibashi Y, Izawa T, Saitoh D, Kinugasa T, Haga S.: "We have two strategies to attain healthy ageing."Geriatr Gerontol Int. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takemasa T, Sugimoto K, Miyazaki M, Machida M, Ikeda S, Hitomi Y, Kizaki T, Ohno H, Yamashita K, Haga S.: "Simple method for the identification of oxidative fibers in skeletal muscle."Eur J Appl Physiol. 91. 357-359 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hitomi Y, Kizaki T, Katsumura T, Mizuno M, Itoh CE, Esaki K, Fujioka Y, Takemasa T, Haga S, Ohno H.: "Effect of moderate acute exercise on expression of mRNA involved in the calcineurin signaling pathway in human skeletal muscle."IUBMB Life. 55. 409-413 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hitomi Y, Miyamura M, Mori S, Suzuki K, Kizaki T, Itoh C, Murakami K, Haga S, Ohno H.: "Intermittent hypobaric hypoxia increases the ability of neutrophils to generate superoxide anion in humans."Clin Exp Pharmacol Physiol. 30. 659-664 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohno H, Kizaki T, Suzuki K, Hitomi Y, Watanabe S, Ookawara T, Suzuki K, Haga S, Saitoh D, Radak Z, Ji LL.: "Physical exercise and extracellular superoxide dismutase : antiatherogenic effects (Review)."Adv Exerc Sports Physiol. 9. 45-53 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueda H, Urano Y, Sakurai T, Kizaki T, Hitomi Y, Ohno H, Izawa T.: "Enhanced expression of neuronal nitric oxide synthase in islets of exercise trained rats."Biochem Biophys Res Commun. 312. 794-800 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki K, Kizaki T, Hitomi Y, Nukita M, Kimoto K, Miyazawa N, Kobayashi K, Ohnuki Y, Ohno H.: "Genetic variation in hypoxia-inducible factor lalpha and its possible association with high altitude adaptation in Sherpas."Med Hypotheses. 61. 385-389 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyazaki H, Haga S, Suzuki K, Toshinai K, Esaki K, Oh-ishi S, Hitomi Y, Kizaki T, Ji LL, Ohno H.: "Effect of eccentric exercise on neutrophil function in human plasma."Adv Exerc Sports Physiol. 9. 69-75 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kizaki T, Suzuki K, Hitomi Y, Taniguchi N, Saitoh D, Watanabe K, Onoe K, Day NK, Good RA, Ohno H.: "Uncoupling protein 2 plays an important role in nitric oxide production of lipopolysaccharide-stimulated macrophages."Proc Natl Acad Sci USA. 99. 9392-9397 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Muto M, Nakane M, Hitomi Y, Yoshida S, Sasaki S, Ohtsu A, Yoshida S, Ebihara S, Esumi H.: "Association between aldehyde dehydrogenase gene polymorphisms and the phenomenon of field cancerization in patients with head and neck cancer."Carcinogenesis. 23. 1759-1765 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato K, Hitomi Y, Imamura K, Esumi H.: "Hyperstable U1snRNA complementary to the K-ras transcripts induces cell death in pancreatic cancer cells."Br J Cancer. 87. 898-904 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ookawara T, Suzuki K, Haga S, Ha S, Chung KS, Toshinai K, Hamaoka T, Katsumura T, Takemasa T, Mizuno M, Hitomi Y, Kizaki T, Suzuki K, Ohno H.: "Transcription regulation of gene expression in human skeletal muscle in response to endurance training."Res Commun Mol Pathol Pharmacol. 111. 41-54 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki K, Mizutani M, Hitomi Y, Kizaki T, Ohno H, Ishida H, Haga S, Koizumi S.: "Association of SH2-B to phosphorylated tyrosine residues in the activation loop of TrkB."Res Commun Mol Pathol Pharmacol. 111. 27-39 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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