2003 Fiscal Year Final Research Report Summary
ROLE OF BRCA1 IN RADIATION-INDUCED CENTROSOMAL CHECKPOINT
| Project/Area Number |
14580569
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
環境影響評価(含放射線生物学)
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| Research Institution | Nagasaki University |
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Principal Investigator |
SUZUKI Kenji Nagasaki University, SCHOOL OF BIOMEDICAL SCIENCES, Research Associate, 大学院・医歯薬学総合研究科, 助手 (00196809)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Masami Nagasaki University, SCHOOL OF BIOMEDICAL SCIENCES, Professor, 大学院・医歯薬学総合研究科, 教授 (20111768)
KODAMA Seiji Nagasaki University, SCHOOL OF BIOMEDICAL SCIENCES, Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (00195744)
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| Project Period (FY) |
2002 – 2003
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| Keywords | RADIATION / BRCA1 / CENTEROSOME / PHOSPHORYLATION |
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| Research Abstract |
IONIZING RADIATION 'HAS BEEN KNOWN TO INDUCED DNA DOUBLE STRAND BREAKS, WHICH RESULT IN ACTIVATION OF ATM. ACTIVATED ATM PHOSPHORYLATES VARIOUS PROTEINS INCLUDING BRCA1. BRCA1 HAS BEEN FOUND TO BE INVOLVED IN INHERITED MAMMARY CARCINOMA, AND IT IS THOUGHT TO HAVE A FUNCTION IN REPAIR OF DNA DOUBLE STRAND BREAKS. HERE, WE SHOW THAT BRCA1 COLOCALIZE WITH CENTROSOMAS. WITHOUT BRCA1 CENTROSOMES TEND TO OVERAMPLIFY, WHICH LEAD TO MULTIPOLAR DIVISION. FURTHERMORE, WE FOUND THAT ACTIVATED FORMS OF ATM, PHOSPHORYLATED ATM AT SERINE1981, ALSO COLOCALIZED WITH CENTROSOMES, WHICH INDICATE THAT ACTIVATED ATM CONSTITUTIVELY PHOSPHORYLATE BRCA1. THESE RESULTS INDICATE THAT ATM-MEDIATED PHOSPHORYLATION OF BRCA1 MAY REGULATE CENTROSOMAL DUPLICATION NOT ONLY IN _UNIRRADIATED CELLS BUT ALSO AFTER IRRADIATION.
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