2005 Fiscal Year Final Research Report Summary
Development of photo-driven motor proteins using phtochromic molecules
| Project/Area Number |
14580677
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Soka University |
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Principal Investigator |
MARUTA Shinsaku Soka University, Dept.of Bioinformatics, Professor, 工学部, 教授 (40231732)
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| Project Period (FY) |
2002 – 2005
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| Keywords | molecular motor / myosin / kinesin / photochromic molecule / azobenzene / laser / energy transduction / ATPase |
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| Research Abstract |
ATP driven-motor proteins transduce chemical energy generated from ATP hydrolysis into the physical motion energy with the mechanical system like cam-shaft. It is expected that introduction of artificial energy input device into the energy transducing site of motor protein, enable the driving or regulating of motor proteins without ATP. In the present study, photochromic molecules which change their conformation reversibly with high response due to the isomerization by ultraviolet (UV) and visible light (VIS) irradiation, have been utilized as energy input device. Especially azobenzene and spiropyran derivatives, which change their characteristics dramatically on isomerization by UV-VIS light irradiation, might work effectively.
In this study, several kinds of azobenzene and spiropyran derivatives, which can bind to cysteine side chain covalently, have been synthesized. And several motor protein mutants, which have one reactive cysteine at the specific site, have been also prepared. It has been demonstrated that for skeletal muscle myosin motor domain, the localized conformational changes resulting from isomerization on azobenzene derivative cross-linking of reactive cysteine residues, SH1 and SH2 induced swing in the lever arm portion. It was shown that the incorporated photochromic group acts as a mechanical energy input in a motor protein. The new technique using photochromic molecules may be applicable to other energy transducing and regulatory proteins.
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Research Products
(12 results)
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[Journal Article] Three-dimensional structural analysis of individual myosin heads undervarious functional states2004
Author(s)
Katayama, E., Ichise, N., Yaeguchi, N., Yoshizawa, T., Maruta, S., Baba, N.
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Journal Title
Adv. In Exp. Med. And Biol. 538
Pages: 295-304
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Incorporation of an azobenzene derivative into the energy transducing site of skeletal muscle myosin results in photo-induced conformational changes2004
Author(s)
Umeki, N., Yoshizawa, T., Sugimoto, Y., Mitsui, T., Wakabayashi, K., Maruta, S.
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Journal Title
Journal of Biochemistry 136
Pages: 839-846
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Three-dimensional structural analysis of individual myosin heads undervarious functional states2004
Author(s)
Katayama, E., Ichise, N., Yaeguchi, N., Yoshizawa, T., Maruta, S., Baba, N.
-
Journal Title
Adv.In Exp.Med.And Biol. 538
Pages: 295-304
Description
「研究成果報告書概要(欧文)」より
-
-
[Journal Article] Incorporation of an azobenzene derivative into the energy transducing site of skeletal muscle myosin results in photo-induced conformational changes2004
Author(s)
Umeki, N., Yoshizawa, T., Sugimoto, Y., Mitsui, T., Wakabayashi, K., Maruta, S.
-
Journal Title
J.Biochem. 136
Pages: 839-846
Description
「研究成果報告書概要(欧文)」より
