2003 Fiscal Year Final Research Report Summary
Translational and rotational positioning of nucleosomes and transcriptional regulation
| Project/Area Number |
14580679
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Tohoku University |
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Principal Investigator |
KOMURA Jun-ichiro Tohoku University, Graduate School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (10215410)
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| Project Period (FY) |
2002 – 2003
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| Keywords | chromatin / nucleosome / ionizing radiation |
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| Research Abstract |
1.I have developed a new method for determining, with resolution at the nucleotide level, the rotational positions of nucleosomes along single-copy genes in mammalian cells, by combining X-irradiation with ligation-mediated PCR.
2.I have analyzed the influence of the transcriptional activation of the c-FOS gene on a nucleosome positioned in the promoter of this gene. When human fibroblasts were serum-induced, no change in the position of this nucleosome was detected, although concurrent chemical modifications of nucleosomes in the c-FOS region upon induction have been reported.
3.I have studied cell cycle-dependent changes in the positioning of nucleosomes in the promoters of the c-FOS gene and the RNU6 gene. In mitotic HeLa cells, the nucleosome positioning in these promoters appeared to be lost. This result suggests random distribution of nucleosomes in mitotic chromososmes.
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