2003 Fiscal Year Final Research Report Summary
THE ROLE OF THE TRANSLATIONAL REGULATOR NAT1 IN DEVELOPMENT
| Project/Area Number |
14580714
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | NARA INSTITUTE OF SCIENCE AND TECHNOLOGY |
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Principal Investigator |
YAMANAKA Shinya NARA INSTITUTE OF SCIENCE AND TECHNOLOGY, CENTER FOR GENETIC INFORMATION, PROFESSOR, 遺伝子教育研究センター, 教授 (10295694)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Makoto NATIONAL INSTITUTE FOR BASIC BIOLOGY, DIVISION FOR MORPHOGENESIS, RESEARCH ASSOCIATE, 基礎生物学研究所, 助手 (30212103)
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| Project Period (FY) |
2002 – 2003
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| Keywords | Translational control / non-AUG / ES cell / Paralogue |
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| Research Abstract |
Only a few cases of exclusive translation initiation at non-AUG codons have been reported. We recently demonstrated that mammalian NAT1 mRNA, encoded by E1F4G2, uses GUG as its only translation initiation codon. In this study, we identified NAT1 orthologs from chicken, Xenopus, and zebrafish and found that in all species, the GUG codons also serve as the initiation codon. In all species, the GUG codons fulfilled the reported requirements for non-AUG initiation : an optimal Kozak motif and a downstream hairpin structure. Site-directed mutagenesis showed that nucleotides at positions -3 and +4 are critical for the GUG-mediated translation initiation in vitro. We found that NAT1 orthologs in Drosophila melanogaster and Halocynthia roreizi also use non-AUG start codons, demonstrating evolutional conservation of the non-canonical translation initiation.
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Research Products
(3 results)
