2003 Fiscal Year Final Research Report Summary
Molecular mechanisms underlying larval tail regenration in Xenopus Laevis
| Project/Area Number |
14580716
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Himeji Institute of technology |
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Principal Investigator |
MOCHII Makoto Himeji Institute of Technology, Graduate School of Science, Associate professor, 大学院・理学研究科, 助教授 (90202358)
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| Project Period (FY) |
2002 – 2003
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| Keywords | Xenopus laevis / larva / tail regeneration / gene expression / cell labeling / cell differentiation / cDNA array |
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| Research Abstract |
This research was performed to understand molecular mechanisms underlying organ regeneration using larval tail of Xenopus laevis as a model system. The following results were obtained. 1.Cell labeling experiment using bromodeoxyuridine and morphological observation suggested that spinal cord, notochord and muscle tissue in the regenerated tail were formed with cells derived from each tissue. 2.Gene expression analysis by in situ hybridization and RT-PCR methods revealed that many genes were expressed in both the tail development and the tail regeneration processes while some genes, for example, chordin and noggin were not expressed in the tail regeneration process. 3.In situ hybridization and functional analysis using animal cap suggested a presence of a distal to proximal gradient of Xwnt-5a activity in the regenerating tail. Ectopic expression of Xwnt-5a in a dorsal incision on larval tail induced an ectopic tail formation, suggested an instructive role of Xwnt-5a in anterior-posterior pattering of the tail regeneration. 4.Differential hybridization using a cDNA array including cDNAs derived from the regenerating tail resulted in identification of eight genes up-regulated in the tail regeneration process.
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