DENDRITE-ASSOCIATED CELL ABHESION MOLECULE OBCAM IN THE HYPOTHALAMIC MAGNOCELLULAR NEURONS

2003 Fiscal Year Final Research Report Summary

• Project/Area Number: 14580729
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: KYOTO INSTITUTE OF TECHNOLOGY
• Principal Investigator: MIYATA Seiji KYOTO INSTITUTE OF TECHNOLOGY, DEPARTMENT OF APPLIED BIOLOGY, ASSISTANT PROFESSOR, 工芸科学研究科, 助手 (30243124)
• Project Period (FY): 2002 – 2003
• Keywords: dendrite / targetting / brain / neurohypophysis / plasticity / cell adhesion molecule / oxytocin / vasopressin

Research Abstract

Opioid-binding cell adhesion molecule (OBCAM) is one of immunoglobulin superfamily cell adhesion molecule and consists of three IgG-like domains. In the present study, it was shown that OBCAM located specifically at the dendrites of magnocellular neurons of the hypothalamic supraoptic and paraventricular nucleus. Electron microscopic observation further showed that OBCAM was localized at vasopressin-secreting neurosecretory granules of the dendrites and released together with dendritic release of vesicles. In cultured cortical neurons, OBCAM was also observed to target to their dendritic shaft specifically at postsynaptic dendritic spine. Electron microscopic observation revealed that OBCAM was seen to accumulate at postsynaptic membrane and density. Cross-linking study demonstrated that OBCAM was able to bind homophilically each other and heterophilically with Kilon, another immunoglobulin superfamily member protein.

Research Products

• [Publications] Miyata S., Hatton G.I.: "Activity-related, dynamic neuron-glial interactions in the hypothalamo-neurohypophysial system"Microscopic Research and Technique. 56. 143-157 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Miyata S, et al.: "The chondroitin sulfate proteoglycan phosphacan/RPTPβ in the hypothalamic magnocellular nuclei"Brain Research. 949. 112-121 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Matsunaga W, Miyata S, et al.: "Expression of high levels of tubulin and microtubule-associated protein-2 in the neurohypophysisal astrocytes of adult rats"Neuroscience. 111. 151-162 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Miyata S, et al.: "Biochemical and ultrastructural analysis of IgLON cell adhesion molecules, Kilon and OBCAM in the rat brain"Neuroscience. 117. 645-658 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Miyata S, et al.: "Dendrite-associated cell adhesion OBCAM localizes at neurosecretory granules in the hypothalamic magnocellular neurons"Neuroscience. 122. 169-181 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Miyata S, et al.: "Polarized targetting of IgLON cell adhesion molecule OBCAM to dendrites in cultured neurons"Brain Research. 979. 123-136 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Maekawa S, Iino T, Miyata S: "Molecular characterization of the detergent-insoluble cholesterol-rich membrane microdomain (raft) of the central nervous system"Biochemica et Biophysica Acta Biomembranes. 1610. 261-270 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hayashi N, Miyata S, et al.: "Attenuation of glial scar formation in the injured rat brain by heparin oligosaccharides"Neuroscience Research. In press. (2004)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Miyata S, et al.: "Activity-dependent regulation of a chondroitin sulfate proteoglycan 6B4 phosphacan/RPTPβ in the hypothalamo-neurohypophysial system"Neuroscience. In press. (2004)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Miyata, S., Hatton, G.I.: "Activity-related, dynamic neuron-glial interactions in the hypothalamo-neurohypophysial system."Microscopy Research and Technique. 56. 143-157 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Miyata, S., Shinga, I., Taguchi, K., Nakashima, T., Kiyohara, T., Oohira,A.: "The chondroitin sulfate proteoglycan phosphacan/RPTPβ in the hypothalamic magnocellular nuclei."Brain Research. 949. 112-121 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Matsunaga, W., Miyata, S., Itoh, M., Kiyohara, T., Maekawa,S.: "Expression of high levels of tubulin and microtubule-associated protein-2 in the neurohypophysial astrocytes of adult rat."Neuroscience. 111. 151-162 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Miyata, S., Matsumoto, N., Taguchi, K., Akagi, A., Iino, T., Funatsu, N., Maekawa,S.: "Biochemical and ultrastructural analysis of IgLON cell adhesion molecules, Kilon and OBCAM in the rat brain."Neuroscience. 117. 645-658 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Miyata, S., Matsumoto, N., Maekawa, S.: "Polarized targeting of IgLON cell adhesion molecule OBCAM to dendrites in cultured in cultured neurons."Brain Research. 979. 123-136 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Miyata, S., Taguchi, K., Maekawa, S.: "Dendrite-associated cell adhesion molecule OBCAM localizes at neurosecretory granules in the hypothalamic magnocellular neurons."Neuroscience. 122. 169-181 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Maekawa, S., Iino, S., Miyata, S.: "Molecular characterization of the detergent-insoluble cholesterol-rich membrane microdomain (raft) of the central nervous system"Biochimica et Biophysica Acta Biomembranes. 1610. 261-270 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hayashi, N., Miyata, S., Kariya, Y., Takano, R., Hara, S., Kamei,K.: "Attenuation of glial scar formation in the injured rat brain by heparin oligosaccharides."Neuroscience Research. (in press). (2004)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Miyata, S., Akagi, A., Hayashi, N., Watanabe, K., Oohira,A.: "Activity-dependent regulation of a chondroitin sulfate proteoglycan 6B.4 phosphacan/RPTPβ in the hypothalamo-neurohypophysial system."Neuroscience. (in press). (2004)
  • Description: 「研究成果報告書概要(欧文)」より