2003 Fiscal Year Final Research Report Summary
Molecular Analysis of a novel PDZ protein interacting with orphan glutamate receptorδ2
| Project/Area Number |
14580747
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Yokohama City University |
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Principal Investigator |
KAWAMOTO Susumu Yokohama City University, Department of Bacteriology, Associate Professor, 医学部, 講師 (80125921)
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| Project Period (FY) |
2002 – 2003
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| Keywords | Glutamate receptor / δ2 subunit / Scaffolding protein / PDZ domain / FH domain / Surface plasmon resonance / Delphilin / Monocarboxylate transporter |
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| Research Abstract |
Delphilin, GluRδ2-interacting protein, may serve to link GluRδ2 with various signaling molecules. In the present study, we used surface plasmon resonance analysis to screen molecules interacting with Delphilin PDZ domain for elucidating its function. Candidate molecules were : ionotropic GluRs, metabotropic GluRs and other related molecules, which are known to be localized in cerebellar Purkinje cells and to have C-terminal sequence similar to that of GluRδ2 molecules. Among these molecules, monocarboxylate transporter (MCT) 2 was found to be a candidate molecule interacting with Delphilin PDZ domain. Furthermore, we are also ezploring the possibility that some signaling molecule(s) may interact with the Delphilin FH1 domain.
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