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2003 Fiscal Year Final Research Report Summary

Investigation for molecular mechanisms by which the retinal ganglion cells perceive carbohydrate chains of chondroitin sulfate proteaglycans (chondroitin sulfate) as axonal guidance cues

Research Project

Project/Area Number 14580762
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionUniversity of Tsukuba, Institute of Basic Medical Sciences

Principal Investigator

ICHIJO Hiroyuki  University of Tsukuba, Institute of Basic Medical Sciences, Assistant Professor, 基礎医学系, 講師 (40272190)

Project Period (FY) 2002 – 2003
Keywordsretinal ganglion cell / axon / growth cone / chondroitin sulfate / D unit chondroitin sulfate / carbohydrate chain / retinotectal projection
Research Abstract

Axons of retinal ganglion cells do not invade the telencephalon anteriorly. Our previous researches have shown that the telencephalic cells secrete chondroitin sulfate proteoglycans (CSPGs), and that their carbohydrate chains (chondroitin sulfates, CSs) prevent the retinal axons from invading the telencephalic territory in chick embryos.
CSs exhibit their structural diversity ; the D unit rich CSs was distributed in the diencephalotelencephalic boundary. In vitro, spot of the D unit CS on the cultural substrate prevented the retina axons from invading ; however, soluble D unit CS in the culture medium has no effect on the axon outgrowth but inhibit the effect of D unit spot.
To know roles of D unit CS in formation of the retinal trajectory in vivo, solution of the D unit CS was injected into lateral ventricle of the embryos ; anterior border of the optic tract shifted toward the telencephalon and the retinal axons entered the telencephalic territory aberrantly.
The results indicate that structural diversity of the CSs serves their functional specificity in axon wiring. The retinal axons distinguish the D unit CS from other types of CSs, suggesting mechanisms perceiving the CSs' diversity.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Hiroyuki Ichijo: "Roles of Proteoglycans in Neuronal Circuit Formation of Retinotectal Projections"Connective Tissue. 35. 11-17 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroyuki Ichijo: "Proteoglycans as cues for axonal guidance in the formation of retinotectal or retinocollicular projections"Molecular Neurobiology. in press.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroyuki Ichijo: "Roles of Proteoglycans in Neuronal Circuit Formation of Retinotectal Projections"Connective Tissue. vol.35. 11-17 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroyuki Ichijo: "Proteoglycans as cues for axonal guidance in the formation of retinotectal or retinocollicular projections"Molecular Neurobiology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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