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2003 Fiscal Year Final Research Report Summary

Study of glial cell function during brain ischemia

Research Project

Project/Area Number 14580792
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 神経・脳内生理学
Research InstitutionHyogo College of Medicine

Principal Investigator

YAMAMOTO Satoshi  Hyogo College of Medicine, Faculty of Medicine, Associate Professor, 医学部, 助教授 (60220464)

Project Period (FY) 2002 – 2003
KeywordsGlial cell / Astrocyte / Brain ischemia / Patch-clamp / Membrane current / Glutamate / Glutamate receptors
Research Abstract

Purpose : Study of functional changes in electrical membrane properties and chemical receptivity of astrocytes during in vitro ischemia.
Method : Using whole-cell patch-clamp techniques, membrane currents were monitored from single cultured astrocyte, and the effects of in vitro ischemia on the electrical membrane properties and receptivity for neurotransmitter, glutamate were examined. Ischemic condition was made by perfusing cells with glucose-free and metabolic inhibitor-containing solution instead of normal artificial cerebrospinal fluid.
Results : Ischemic solution caused a transient inward current followed by a slow outward current in the membrane of astrocytes recorded. From the reversal potential, it was suggested that chloride channels and potassium channels might be involved in the generation of the transient inward currents and the slow outward currents, respectively. Glutamate caused inward currents in the astrocyte. The glutamate-induced currents were inhibited by NMDA and non-NMDA glutamate receptor antagonists by about 50%, indicating that astrocytes have not only ionotropic glutamate receptor but also metabotropic glutamate receptors and/or glutamate transporters. The glutamate-induced currents were not modified by ischemic condition in the early time. Due to difficulties of continuous recording, the late effect of ischemia could not be monitored.
Conclusion : The astrocyte that has been seemed to be a ischemia-resistant cell, behaves like as the neuron in the electrical membrane properties against ischemia. This suggests that glial cell function can be altered during ischamia, so that maintenance of extracellular concentrations of potassium and neurotransmitters by glial cells might be disturbed.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Niiyama, S., Yamamoto, S.他: "Bupivacaine, but not tetracaine, protects against the in vitro ischemic insult of rat hippocampal CA1 neurons."Neurosci.Res.. 42. 231-241 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohta, K., Yamamoto, S.他: "L-trans-PDC enhances hippocampal neuronal activity by stimulating glial glutamate release independently of blocking transporters."Biochem.Biophys.Res.Commun.. 295. 376-381 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohta, K., Yamamoto, S.他: "Stearic acid facilitates hippocampal neurotransmission by enhancing nicotinic ACh receptor responses via a PKC pathway."Brain Research Mol. Brain Res.. 119. 83-89 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saitoh.M., Yamamoto, S.他: "Arachidonic acid peroxides induce apoptotic Neuro-2A cell death in association with intracellular Ca^<2+> rise and mitochondrial damage independently of caspase-3 activation."Brain Research. 991. 187-194 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyamoto.H., Yamamoto, S.他: "2-pyrrolidinone induces a long-lasting facilitation of hippocampal synaptic transmission by enhancing alpha7 ACh receptor responses via a PKC pathway."Brain Research Mol. Brain Res.. 117. 91-96 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Maruo, K., Yamamoto, S.他: "Tunicamycin inhibits NMDA and AMPA receptor responses independently of N-glycosylation."Brain Research. 977. 294-297 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Niiyama, S., Yamamoto, S.et al.: "Bupivacaine, but not tetracaine, protects against the in vitro ischemic insult of rat hippocampal CA1 neurons."Neurosci.Res.. 42. 231-241 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tozaki, H., Yamamoto, S.et al.: "The inhibitory and facilitatory actions of amyloid-β peptides on nicotinic ACh receptors and AMPA receptors."Biochem.Biophys.Res.Commun.. 294. 42-45 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohta, K., Yamamoto, S.et al.: "L-trans-PDC enhances hippocampal neuronal activity by stimulating glial glutamate release independently of blocking transporters."Biochem.Biophys.Res.Commun.. 295. 376-381 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagai, K., Yamamoto, S.et al.: "(-)-Epigallocatechin gallate protects against NO stress-induced neuronal damage after ischemia by acting as an anti-oxidant."Brain Res.. 956. 319-322 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Maruo, K., Yamamoto, S.et al.: "Tunicamycin inhibits NMDA and AMPA receptor responses independently of N-glycosylation."Brain Res.. 977. 294-297 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumoto, S., Yamamoto, S.et al.: "Pipecolic acid induces apoptosis in neuronal cells."Brain Res.. 980. 179-184 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyamoto, H., Yamamoto, S.et al.: "2-pyrrolidinone induces a long-lasting facilitation of hippocampal synaptic transmission by enhancing alpha? ACh receptor responses via a PKC pathway."Brain Res.Mol.Brain Res.. 117. 91-96 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saitoh, M., Yamamoto, S.et al.: "Arachidonic acid peroxides induce apoptotic Neuro-2A cell death in association with intracellular Ca^<2+> rise and mitochondrial damage independently of caspase-3 activation."Brain Res.. 991. 187-194 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohta, K., Yamamoto, S.et al.: "Stearic acid facilitates hippocampal neurotransmission by enhancing nicotinic ACh receptor responses via a PKC pathway."Brain Res.Mol.Brain Res.. 119. 83-89 (2003)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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