2003 Fiscal Year Final Research Report Summary
Advanced effect of rh-FGF on properties and manufacturing conditions of cultured skin substitute
| Project/Area Number |
14580828
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | KITASATO UNIVERSITY |
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Principal Investigator |
KUROYANAGI Yoshimitsu School of Allied Health Sciences, R&D Center for Artificial Skin, Professor, 医療衛生学部, 教授 (80170140)
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| Project Period (FY) |
2002 – 2003
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| Keywords | fibroblasts growth factor / faibroblasts / allogeneic cultured dermal subsitute |
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| Research Abstract |
We developed the cultured dermal substitute (CDS) composed of a two-layered spongy matrix of hyaluronic acid and atelo-collagen containing fibroblasts. This type of CDS is designed to provide an effective therapy for victims with burn and ulcer wounds. The aim of this study is to evaluate the efficacy of recombinant human acidic FGF (aFGF) on the potency of fibroblasts in the two-layered spongy matrix to release vascular endothelial growth factor. At first, the effect of aFGF on the proliferation of fibroblasts in a culture flask was tested to confirm the safety and efficacy. Addition of aFGF was found to increase in cell number and decrease in cellular volume. Decrease of cellular volume does not represent an abnormal morphology but simply reflect a more rapid rate of cell division. These responses of fibroblasts with aFGF were marked but transient, because they returned the original proliferation rate and size in cellular volume after further cultivation in the medium without aFGF The CDS prepared through cultivation of fibroblasts on the two-layered spongy matrix in a medium containing aFGF. The addition of aFGF was found to increase in cell number and consequently increase the amount of VEGF released from fibroblasts.
The CDS composed of the two-layered spongy matrix of hyaluronic acid and atelo-collagen containing fibroblasts was fabricated through cultivation in medium containing aFGF. This CDS is able to keep the high cell viability and potency to release VEGF that plays an important role in wound healing such as granulation tissue formation, matrix formation, and angiogeneis.
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