2014 Fiscal Year Annual Research Report
微細加工技術を用いた分化心筋細胞からの未分化ヒト多能性幹細胞除去システムの開発
| Project/Area Number |
14F04046
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| Research Institution | Kyoto University |
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Principal Investigator |
中辻 憲夫 京都大学, 物質-細胞統合システム拠点, 教授 (80237312)
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| Co-Investigator(Kenkyū-buntansha) |
LI Junjun 京都大学, 物質-細胞統合システム拠点, 外国人特別研究員
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| Project Period (FY) |
2014-04-25 – 2016-03-31
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| Keywords | ヒト多能性幹細胞 / Cardiomyocyte / Microfluidic / Capture |
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| Outline of Annual Research Achievements |
「研究目的」：
In this project, we aim to develop a robust and high efficient system for removal of undifferentiated cells from hPSC-derived cardiomyocytes to eliminate the risk of tumorigenesis in a patient. We therefore propose to use the method we previously developed for capturing rare cells, such as EPCs and CTCs in blood. Specifically, we planned to apply a microfluidic device with fishing net-like micro-pillar arrays in efficient capture of undifferentiated hPSCs in a solution of hPSCs-derived cardiomyocytes. For the second phase, we will integrate nano-engineered substrates, such as nanofibers into our capturing devices to increase the efficiency to remove undifferentiated hPSCs.
「研究計画及び実績」: Last year, we have finished the design and the fabrication of the microfluidic chip, and set up a mini, easy to handle platform for the purification of hPSCs-derived cardiomyocytes. We then proved that the viability of cardiomyocytes after circulating in the microfluidic-chip. The capture rate of certain type of cells can be up to 80%, paper about this work is now under preparation.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Our work in last year have validated the feasibility of removal small amount of undifferentiated cells from hPSC-derived cardiomyocytes. The viability and the capture rate have been both verified. The parameters in the microfluidics, such like flow rate, circulation mode, and the cell types have been characterized. We are close to finish the first aim in our proposal.
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| Strategy for Future Research Activity |
In the next phase, we will finish the systematic validation of the parameters of microfluidics chips to find the most efficient way to remove certain cell types from hPSC-derived cardiomyocytes. We will also begin to use the nanofiber based technique to work as a complementary removal method.
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Research Products
(1 results)
