2015 Fiscal Year Annual Research Report
デングウイルス感染蚊の網羅的遺伝子発現と新規ベクタ－コントロール
| Project/Area Number |
14J12097
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ルントゥウェネ L.R. 東京大学, 新領域創成科学研究科, 特別研究員(PD)
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| Project Period (FY) |
2014-04-25 – 2016-03-31
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| Keywords | dengue virus / deep sequencing / l(2)efl / aedes aegypti |
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| Outline of Annual Research Achievements |
On this fiscal year, we tried to analyze the gene in-vivo by injecting l(2)efl-1, -2, -3, and -4 dsRNAs to mosquito thoraxes and then followed by DENV-2 injection 48 hours after dsRNA-injection. We used the same dsRNA that we transfected in CCL-125. We did preliminary experiment to see how long the dsRNA would give an effect in-vivo. We found that 72 hours after dsRNA injection, the level of l(2)efl-1 to l(2)efl-5 was suppressed. Because we needed to consider that after 72 hours dsRNA might be losing its effect, we decided to inject DENV-2 48 hours after dsRNA injection. Following dsRNA-injection, mosquitoes looked healthy with only little number of mosquitoes died. Forty eight hours afterwards, we injected mosquitoes with DENV-2 and sacrificed the mosquitoes 24, 48, and 72 hours later. Unfortunately, using qRT-PCR, we found that there was no significant difference between dsRNA-injected and control mosquitoes.
The reason that DENV-2 kept on replicating might because dsRNA was losing its effect at the time of DENV-2 injection. In the future, we will try to co-inject DENV-2 and dsRNA at the same time or to create a transgenic mosquito by injecting dsRNA at the embryo stage. We have submitted the results of in-vitro experiment to Japanese Journal of Infectious Disease for consideration.
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| Research Progress Status |
27年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
27年度が最終年度であるため、記入しない。
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[Journal Article] Susceptibility of Aedes flavopictus miyarai and Aedes galloisi mosquito species in Japan to dengue type 2 virus2016
Author(s)
Raweewan Srisawat, Thipruethai Phanitchat, Narumon Komalamisra, Naoki Tamori, Lucky Runtuwene, Kaori Noguchi, Kyoko Hayashida, Shinya Hidano, Naganori Kamiyama, Ikuo Takashima, Tomohiko Takasaki, Ichiro Kurae, Narihiro Narita, Takashi Kobayashi, Yuki Eshita
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Journal Title
Asian Pacific Journal of Tropical Biomedicine
Volume: 6
Pages: 1
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] 日本国内への侵入が危惧される節足動物媒介性ウイルスと日本の蚊について2015
Author(s)
江下優樹,福田昌子,Lucky R. Runtuwene,野口香緒里,飛弾野真也,神山長慶,林田京子,小林隆志, Thipruethai Phanitchat,Raweewan Srisawat,Narumon Komalamisra,成田弘成,牛島廣治,倉根一郎,高崎智彦
Organizer
第36回都市有害生物管理学会大会・総会
Place of Presentation
東京農業大学
Year and Date
2015-07-04 – 2015-07-05
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[Presentation] Susceptibility of Japanese Aedes (Stegomyia) mosquitoes to arboviruses2015
Author(s)
Yuki Eshita, Masako Fukuda, Lucky R. Runtuwene, Kaori Noguchi, Shinya Hidano, Naganori Kamiyama, Kyoko Hayashida, Raweewan Srisawat, Narumon Komalamisra, Hironari Narita, Hiroshi Ushijima, Arthur E. Mongan, Josef Tuda, Mihoko Imada, Junya Yamagishi, Chihiro Sugimoto, Ryuichiro Maeda, Yutaka Suzuki, Takashi Kobayashi
Organizer
The 2nd conference on Asian Pediatric Infectious Diseases
Place of Presentation
The University of Tokyo
Year and Date
2015-06-19 – 2015-06-20
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