2003 Fiscal Year Annual Research Report

シロイヌナズナ完全長cDNAマイクロアレイを用いた遺伝子発現解析

Research Project

Project/Area Number	15011264
Research Institution	The Institute of Physical and Chemical Research
Principal Investigator	関原明独立行政法人理化学研究所, 植物分子生物学研究室, 先任研究員 (80281624)
Keywords	シロイヌナズナ / cDNAマイクロアレイ / 完全長cDNA / ストレス応答 / シグナル伝達 / プロモーター
Research Abstract	本研究では、モデル高等植物のシロイヌナズナを用い、シロイヌナズナDNAマイクロアレイを用いた解析により、種々のストレス、ホルモンに応答する遺伝子等を単離、同定する。同定された遺伝子に関してプロモーター解析を行う。これまでに単離した約7000個のシロイヌナズナ完全長cDNAクローンを用いて完全長cDNAマイクロアレイを作製し、乾燥,低温、塩などの種々のストレスまたはABAなどの種々のホルモン処理、種々の変異体やトランスジェニック植物など200種類以上の異なる条件下での発現プロファイル解析を行った。論文発表したものの幾つかに関しては発現データを公開した。アレイ解析により乾燥2時間後の再吸水処理により応答する遺伝子を152個同定した。同定された誘導性遺伝子に関して再吸水処理過程での発現パターンについて解析し、再吸水処理直後には、"細胞の乾燥ストレス状態からの解放"に関わる遺伝子が機能し、やがて、後期になって、"光合成や植物生長などの再開"に関わる遺伝子が機能する事を明らかにした。再吸水処理誘導性遺伝子の中には、乾燥ストレスにより発現誘導されるもの、発現が抑制されるもの、発現に変化がないものの3つのグループが存在することを明らかにした。再吸水処理誘導性遺伝子と無処理植物体を単に水に移すという処理で発現誘導される遺伝子とをベン図解析して、再吸水処理誘導性遺伝子の中には1)単に水に移すという処理でも発現誘導される遺伝子(細胞内の浸透圧変化の調節に関与するもの)と、2)単に水に移すという処理では発現誘導されない遺伝子(乾燥ストレスにより生じたダメージの修復に関与するもの)、の2つのグループが存在することを明らかにした。さらに再吸水処理誘導性遺伝子のプロモーター解析の結果、再吸水処理による発現誘導に関与する新規なシス配列の存在が示唆された。

Research Products
(22 results)

All Other

All Publications (22 results)

[Publications] Seki et al.: "Molecular responses to drought, salinity and frost : common and different paths for plant protection."Current Opinion in Biotechnology. 14. 194-199 (2003)
[Publications] Shinozaki et al.: "Regulatory network of gene expression in the drought and cold stress responses."Current Opinion in Plant Biology. 6. 410-417 (2003)
[Publications] Seki et al.: "RIKEN Arabidopsis full-length (RAFL) cDNA and its application for expression profiling under abiotic stress conditions."Journal of Experimental Botany. 55. 213-223 (2004)
[Publications] 関原明他: "シロイヌナズナ完全長cDNAを用いた植物ゲノムの機能・発現解析"蛋白質・核酸・酵素. 48(14). 1890-1898 (2003)
[Publications] Oono et al.: "Monitoring expression profiles of Arabidopsis gene expression during rehydration process after dehydration using ca.7000 full-length cDNA microarray."Plant Journal. 34. 868-887 (2003)
[Publications] Abe et al.: "Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling."Plant Cell. 15. 63-78 (2003)
[Publications] Dubouzet et al.: "OsDREB genes in rice, Oryza sativa L, encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression."Plant Journal. 33. 751-763 (2003)
[Publications] Simpson et al.: "Two different novel cis-acting elements of erd1, a clpA homologous Arabidopsis gene function in induction by dehydration stress and dark-induced senescence."Plant Journal. 33. 259-270 (2003)
[Publications] Narusaka et al.: "The cDNA microarray analysis using an Arabidopsis pad3 mutant reveals the expression profiles and classification of genes induced by Alternaria brassicicola attack."Plant and Cell Physiology. 44. 377-387 (2003)
[Publications] Kimura et al.: "Identification of Arabidopsis genes regulated by high light stress using cDNA microarray."Photochemistry and Photobiology. 77. 226-233 (2003)
[Publications] Narusaka et al.: "Expression profiles of Arabidopsis phospholipase A IIA gene in response to biotic and abiotic stresses."Plant and Cell Physiology. 44. 1246-1252 (2003)
[Publications] Nanjo et al.: "Toxicity of free proline revealed in an Arabidopsis T-DNA-tagged mutant deficient in proline dehydrogenase."Plant and Cell Physiology. 44. 541-548 (2003)
[Publications] Rabbani et al.: "Monitoring expression profiles of rice (Oryza sativa L.) genes under cold, drought and high-salinity stresses, and ABA application using both cDNA microarray and RNA gel blot analyses."Plant Physiology. 133. 1755-1767 (2003)
[Publications] Urano et al.: "Characterization of Arabidopsis genes involved in biosynthesis of polyamines in abiotic stress responses and developmental stages."Plant Cell and Environment. 26. 1917-1926 (2003)
[Publications] Nishiyama et al.: "Comparative genomics of the Physcomitrella gametophytic transcriptome and Arabidopsis genome : implication for the land plant evolution."Proc.Natl.Acad.Sci.USA. 100. 8007-8012 (2003)
[Publications] Yamada et al.: "Empirical analysis of transcriptional activity in the Arabidopsis genome."Science. 302. 842-846 (2003)
[Publications] Bassel et al.: "Folate synthesis in plants : The p-aminobenzoate branch is initiated by a bifunctional PabA-PabB protein that is targeted to plastids."Proc.Natl.Acad.Sci.USA. (In press). (2004)
[Publications] Takahashi: "Expression and interaction analysis of Arabidopsis Skp1-related genes."Plant and Cell Physiology. 45. 83-91 (2004)
[Publications] Seki et al.: "Plant Responses to Abiotic Stress (edited by Drs. H.Hirt and K.Shinozaki)"Springer-Verlag. 300 (2003)
[Publications] Seki et al.: "Plant Functional Genomics (edited by Dario Leister)"Haworth's Food Products Press(印刷中). (2004)
[Publications] Seki et al.: "Plant Abiotic Stress (edited by Drs.M.A.Jenks and P.M.Hasegawa)"Blackwell Publishing Ltd.(印刷中). (2004)
[Publications] 関原明他: "ゲノミクス・プロテオミクスの新展開(今中忠行監修)"株式会社エヌ・ティー・エス(印刷中). (2004)

2003 Fiscal Year Annual Research Report

シロイヌナズナ完全長cDNAマイクロアレイを用いた遺伝子発現解析

Principal Investigator

関 原明 独立行政法人理化学研究所, 植物分子生物学研究室, 先任研究員 (80281624)

Research Products

[Publications] Seki et al.: "Molecular responses to drought, salinity and frost : common and different paths for plant protection."Current Opinion in Biotechnology. 14. 194-199 (2003)

[Publications] Shinozaki et al.: "Regulatory network of gene expression in the drought and cold stress responses."Current Opinion in Plant Biology. 6. 410-417 (2003)

[Publications] Seki et al.: "RIKEN Arabidopsis full-length (RAFL) cDNA and its application for expression profiling under abiotic stress conditions."Journal of Experimental Botany. 55. 213-223 (2004)

[Publications] 関 原明 他: "シロイヌナズナ完全長cDNAを用いた植物ゲノムの機能・発現解析"蛋白質・核酸・酵素. 48(14). 1890-1898 (2003)

[Publications] Oono et al.: "Monitoring expression profiles of Arabidopsis gene expression during rehydration process after dehydration using ca.7000 full-length cDNA microarray."Plant Journal. 34. 868-887 (2003)

[Publications] Abe et al.: "Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling."Plant Cell. 15. 63-78 (2003)

[Publications] Dubouzet et al.: "OsDREB genes in rice, Oryza sativa L, encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression."Plant Journal. 33. 751-763 (2003)

[Publications] Simpson et al.: "Two different novel cis-acting elements of erd1, a clpA homologous Arabidopsis gene function in induction by dehydration stress and dark-induced senescence."Plant Journal. 33. 259-270 (2003)

[Publications] Narusaka et al.: "The cDNA microarray analysis using an Arabidopsis pad3 mutant reveals the expression profiles and classification of genes induced by Alternaria brassicicola attack."Plant and Cell Physiology. 44. 377-387 (2003)

[Publications] Kimura et al.: "Identification of Arabidopsis genes regulated by high light stress using cDNA microarray."Photochemistry and Photobiology. 77. 226-233 (2003)

[Publications] Narusaka et al.: "Expression profiles of Arabidopsis phospholipase A IIA gene in response to biotic and abiotic stresses."Plant and Cell Physiology. 44. 1246-1252 (2003)

[Publications] Nanjo et al.: "Toxicity of free proline revealed in an Arabidopsis T-DNA-tagged mutant deficient in proline dehydrogenase."Plant and Cell Physiology. 44. 541-548 (2003)

[Publications] Rabbani et al.: "Monitoring expression profiles of rice (Oryza sativa L.) genes under cold, drought and high-salinity stresses, and ABA application using both cDNA microarray and RNA gel blot analyses."Plant Physiology. 133. 1755-1767 (2003)

[Publications] Urano et al.: "Characterization of Arabidopsis genes involved in biosynthesis of polyamines in abiotic stress responses and developmental stages."Plant Cell and Environment. 26. 1917-1926 (2003)

[Publications] Nishiyama et al.: "Comparative genomics of the Physcomitrella gametophytic transcriptome and Arabidopsis genome : implication for the land plant evolution."Proc.Natl.Acad.Sci.USA. 100. 8007-8012 (2003)

[Publications] Yamada et al.: "Empirical analysis of transcriptional activity in the Arabidopsis genome."Science. 302. 842-846 (2003)

[Publications] Bassel et al.: "Folate synthesis in plants : The p-aminobenzoate branch is initiated by a bifunctional PabA-PabB protein that is targeted to plastids."Proc.Natl.Acad.Sci.USA. (In press). (2004)

[Publications] Takahashi: "Expression and interaction analysis of Arabidopsis Skp1-related genes."Plant and Cell Physiology. 45. 83-91 (2004)

[Publications] Seki et al.: "Plant Responses to Abiotic Stress (edited by Drs. H.Hirt and K.Shinozaki)"Springer-Verlag. 300 (2003)

[Publications] Seki et al.: "Plant Functional Genomics (edited by Dario Leister)"Haworth's Food Products Press(印刷中). (2004)

[Publications] Seki et al.: "Plant Abiotic Stress (edited by Drs.M.A.Jenks and P.M.Hasegawa)"Blackwell Publishing Ltd.(印刷中). (2004)

[Publications] 関 原明 他: "ゲノミクス・プロテオミクスの新展開(今中忠行 監修)"株式会社エヌ・ティー・エス(印刷中). (2004)

関原明独立行政法人理化学研究所, 植物分子生物学研究室, 先任研究員 (80281624)

[Publications] 関原明他: "シロイヌナズナ完全長cDNAを用いた植物ゲノムの機能・発現解析"蛋白質・核酸・酵素. 48(14). 1890-1898 (2003)

[Publications] 関原明他: "ゲノミクス・プロテオミクスの新展開(今中忠行監修)"株式会社エヌ・ティー・エス(印刷中). (2004)