Development of NMR methodology for soft interaction of proteins

2008 Fiscal Year Final Research Report

• Project/Area Number: 15083202
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: The University of Tokyo
• Principal Investigator: SHIMADA Ichio The University of Tokyo, 大学院・薬学系研究科, 教授 (70196476)
• Co-Investigator(Kenkyū-buntansha): TERASAWA Hiroaki 東京大学, 大学院・薬学系研究科, 助手 (10300956)
• Project Period (FY): 2003 – 2008
• Keywords: 膜タンパク質 / 相互作用 / 核磁気共鳴法

Research Abstract

本研究では生体機能の発現に重要かつ創薬開発における標的タンパク質である膜タンパク質を研究対象とし、その機能解明を行うことが可能な核磁気共鳴法の測定法開発を行い、複数の系において機能解明を行った。

Research Products

• [Journal Article] Cross-saturation and transferred cross-saturation experiments (2009)
  • Author(s): Ichio Shimada, Takumi Ueda, Masahiko Matsumoto, Masayoshi Sakakura, Masanori Osawa, Koh Takeuchi, Noritaka Nishida, Hideo Takahashi
  • Journal Title: Prog. Nuc. Magn. Reson. Spect. 54 Pages: 123-140
  • Peer Reviewed
• [Journal Article] Structural Basis of the Collagen-binding Mode of Discoidin Domain Receptor 2 (2007)
  • Author(s): Osamu Ichikawa, Masanori Osawa, Noritaka Nishida, Naoki Goshima, Nobuo Nomura, and Ichio Shimada
  • Journal Title: EMBO J. 26 Pages: 4168-4176
  • Peer Reviewed
• [Journal Article] Ligand-induced structural change of the CD44 hyaluronan-binding domain revealed by NMR (2006)
  • Author(s): Mitsuhiro Takeda, Shinji Ogino, Ryo Umemoto, Masayoshi Sakakura, Masahiro Kajiwara, Kazuki Sugahara, Hiroto Kawashima, Masayuki Miyasaka, Hiroaki Terasawa, and Ichio Shimada
  • Journal Title: J Biol Chem. 281 Pages: 40089-40095
  • Peer Reviewed
• [Journal Article] Bead-linked proteoliposomes : a reconstitution method for NMR analyses of membrane protein-ligand interactions (2005)
  • Author(s): Mariko Yokogawa, Koh Takeuchi, and Ichio Shimada
  • Journal Title: J. Am. Chem. Soc. 127 Pages: 12021-12027
  • Peer Reviewed
• [Journal Article] NMR techniques for identifying the interface of a larger protein-protein complex : cross-salutation and transferred cross-saturation experiments. (2004)
  • Author(s): Ichio Shimada
  • Journal Title: Method. Enzymol. 394 Pages: 483-506
  • Peer Reviewed
• [Journal Article] Novel Collagen-Binding Mode of the VWA Domain Determined by a Transferred Cross-Saturation Experiment (2003)
  • Author(s): Noritaka Nishida, Hiromi Sumikawa, Masayoshi Sakakura, Nobuhisa Shimba, Hideo Takahashi, Hiroaki Terasawa, Ei-ichiro Suzuki, Ichio Shimada
  • Journal Title: Nature Struct. Biol. 10 Pages: 53-58
  • Peer Reviewed
• [Presentation] NMR Study on Membrane proteins-ligands (2007)
  • Author(s): 嶋田一夫
  • Organizer: Interactions-Application of Transferred Cross Saturation- AIMECS 07, the 6th International Medicinal Chemistry Symposium
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 20070708-11
• [Presentation] NMR study on protein-protein interactions using paramagnetic probe (2007)
  • Author(s): 嶋田一夫
  • Organizer: International Workshop on Perspectives on Stable Isotope Aided NMR Methods for Protein Structural Analysis
  • Place of Presentation: Osaka, Japan
  • Year and Date: 20070330-31