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2006 Fiscal Year Final Research Report Summary

Design of Cell-Recognizable Nano-device and Application to Biosensing and Tissue-engineering

Research Project

Project/Area Number 15100008
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biological material science
Research InstitutionTokyo Institute of Technology

Principal Investigator

AKAIKE Toshihiro  Tokyo Institute of Technology, Graduate School of Bioscience and Biotechnology, Professor, 大学院生命理工学研究科, 教授 (30101207)

Co-Investigator(Kenkyū-buntansha) WATANABE Toshiyuki  Tokyo University of Agriculture and Technology, Institute of Symbiotic Science and Technology, Professor, 大学院共生科学技術研究部, 教授 (10210923)
HARADA Ichiro  Tokyo Institute of Technology, Graduate School of Bioscience and Biotechnology, Assistant Professor, 大学院生命理工学研究科, 助手 (00361759)
Project Period (FY) 2003 – 2006
KeywordsRegenerative medicine / Tissue engineering / Extracellular matrix / Cellular recognition / Nano-probe / Genetic engineering / Nano-micell / Nano / Micro fabrication
Research Abstract

In our research, we have designed cell-recognizable nano-probe to analyze cellular behavior in vitro and regulate thme as in vivo. Final goal of our project is to create nano-regulated circumstances of the cells in vitro by using designed nano-probe which have a potential for regenerative medicine and cell-targetting diagnosis and therapy. Bionanothechnology are based on the research objectives. Cell-Matrices engineering are established by our group to regulate the functions of ES-cells as well as hepatocytes using newly-engineered sugar-carrying polymers and chimeric proteins such as E-cadherin Fc, EGF-Fc, LIF-Fc and so on. Nano/micro patterning, probes/carriers technology and biomaterials were developed for dynamic measurements and regulations of cells. The nano carriers technology of carbonate apatite was also established for highly efficient delivery of DNAs, RNAs, proteins and drugs into intra-cellar domain.
We have successfully achieved three major goal. They are, 1. Designing nov … More el cell-recognizable probe as genetically-engineered matrices and application to the cell processing, 2. Designing biocompatible nano-carrier system for targeted cell therapy, 3. Designing nano/ micro 3D patterned culture substrate using two photon polymerization method. For first step, we have designed chimeric proteins which owns cell-recognizable domain and IgG-Fc domain. We found Fc domain successfully allows to anchore nano-probe to any kide of hydrophobic substrate ranging from polystyrene dish to the nano-fiobers. For cell-recognizable domain we tested ranging from cell-cell adhesion proteins such E-cadherin and N-cadherin to cytokines such as EGF and LIF. Any type of ligand we design, the cells successfully recognized them and we found they could regulate cells as we designed. For the next step, we found each of genetically engineered such chimeric proteins were possible to use as nano-probes. We have applied the cell-recognizable proteins for drug/ gene delivery system for targeted cell therapy, and for local stimulation to thte cultured cells. We found our nano-probes could achieve each of the goal. Finally, we desinged nano-regulated culture substrate by using two-photon initiate polymerizatin method. In our project, culture substrate could be fabricated with resolution of 180nm, and also cell could be cultured on the substrate. The substrate we fabricated could achieved to analyze cellular dynamic behavior. Less

  • Research Products

    (10 results)

All 2007 2006 2005

All Journal Article (9 results) Book (1 results)

  • [Journal Article] Embryonic undifferentiated cells show scattering activity on a surface coated with immobilized E-cadherin.2007

    • Author(s)
      Nagaoka, M., Ise, H., Harada, I., Koshimizu, U., Maruyama, A., Akaike, T
    • Journal Title

      Biochem. Biophys. Res. Commun. (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Primary hepatocyte survival on non-integrin-recognizable matrices without the activation of Akt signaling.2007

    • Author(s)
      T.Hoshiba, H.Nagahara, C-S.Cho, Y.Tagawa, T.Akaike
    • Journal Title

      Biomaterials 28

      Pages: 1093-1104

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A simple combined floating and anchored collagen gel for enhancing mechanical strength of culture system.2007

    • Author(s)
      I.Harada, T.Akaike
    • Journal Title

      J. Biomed. Mater. Res. 80

      Pages: 123-130

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Embryonic undifferentiated cells show scattering activity on a surface coated with immobilized E-cadherin.2007

    • Author(s)
      Nagaoka, M., Ise, H., Harada, I., Koshimizu, U., Maruyama, A.Akaike, T
    • Journal Title

      Biochem.Biophys.Res.Commun. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A simple combined floating and anchored collagen gel for enhancing mechanical strength of culture system.2007

    • Author(s)
      I.Harada, Kim SG, Cho CS, Kurosawa K, T.Akaike
    • Journal Title

      J.Biomed.Mater.Res. 80

      Pages: 123-30

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effect of photo-immobilization of epidermal growth factor on the cellular behaviors.2006

    • Author(s)
      K.Ogiwara, M.Nagaoka, C-S.Cho, T.Akaike
    • Journal Title

      Biochem. Biophys. Res. Commun. 345

      Pages: 255-259

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] E-cadherin-coated plates maintain pluripotent ES cells without colony formation.2006

    • Author(s)
      M.Nagaoka, U.Koshimizu, S.Yuasa, F.Hattori, H.Chen, T.Tanaka, M.Okabe, K.Fukuda, T.Akaike
    • Journal Title

      PLoS ONE 1

      Pages: e15

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effect of photo-immobilization of epidermal growth factor on the cellular behaviors.2006

    • Author(s)
      K.Ogiwara, M.Nagaoka, C-S.Cho, T.Akaike
    • Journal Title

      Biochem.Biophys.Res.Commun. 345

      Pages: 255-259

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Fabrication of elastic substrates for tissue engineering by two-photon initiated polymerization2005

    • Author(s)
      Kimura N., Watanabe T., Totani K., Oguni A., Harada I., Akaike T.
    • Journal Title

      Nonlinear Optics, Quantum Optics 34

      Pages: 203-206

    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 再生医療の基礎シリーズ4 再生医療のためのバイオエンジニアリング2007

    • Author(s)
      赤池敏宏
    • Total Pages
      226
    • Publisher
      コロナ社
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2008-05-27  

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