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2007 Fiscal Year Final Research Report Summary

Mechanism in recognition of non-self sustained by the interaction between organelles in plant cell

Research Project

Project/Area Number 15108001
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Plant pathology
Research InstitutionOkayama University

Principal Investigator

SHIRAISHI Tomonori  Okayama University, Graduate School of Natural Science and Technology, Professor (10033268)

Co-Investigator(Kenkyū-buntansha) ICHINOSE Yuki  Okayama University, Graduate School of Natural Science and Technology, Professor (50213004)
INAGAKI Yoshishige  Okayama University, Graduate School of Natural Science and Technology, Associate Professor (50280764)
TOYODA Kazuhiro  Okayama University, Graduate School of Natural Science and Technology, Associate Professor (50294442)
Project Period (FY) 2003 – 2007
Keywordsplant cell wall / apyrase (NTPase) / signal transmission / cell cycle-related gene Cdc27B / ER-transnortation-related eene Rer1(6) / defense reaction / MAMPs / WRKY
Research Abstract

In this research, we attempted to clarify the plant defense mechanism in recognition of MAMPs, signal transmission and action of virulence effectors, mediated by the interaction between respective organelles. In this fiscal year, we showed that several molecules interacted with apyrase in plant cell wall by PAGE and TOF/MS/MS analyses. We also presented that MAMPs activated the cell-wall-bound apyrase, resulting in increasing inorganic phosphate that stimulated generation of active oxygen species and expression of defense-related genes. MAMPs also regulated the expression of NbCdc27B, which is a component of M phase in cell cycle. NbCdc27B participated in defense reaction without hypersensitive cell death Microarray analysis indicated that the transcriptional activation of WRKY41 was induced by MAMPs. Transformants highly expressed WRKY41 showed resistance to Pseudomonas syringae, but, inversely, susceptiblity to Erwinia carotovara, indicating that WRKY41 participated in SA signaling pathway. Transportation of proteins via endoplasmic reticulum may be also crucial to defense. Based on these findings, we discuss defense signaling sustained by respective organelles.

  • Research Products

    (44 results)

All 2008 2007

All Journal Article (23 results) (of which Peer Reviewed: 9 results) Presentation (16 results) Book (5 results)

  • [Journal Article] Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana2008

    • Author(s)
      Higashi, et. al.
    • Journal Title

      Mol. Genet. Genomics 279

      Pages: 303-312

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Gac two-component system in Pseudomonas syringae pv. tabaci is required for virulence but not for hypersensitive reaction2008

    • Author(s)
      Marutani, et. al.
    • Journal Title

      Mol. Genet. Genomics 279

      Pages: 312-322

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] The model plant Medicago truncatula exhibits biparental plastid inheritance2008

    • Author(s)
      Matsushima, et. al.
    • Journal Title

      Plant Cell Physiol. 49

      Pages: 81-89

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Role of flagellin glycosylation in bacterial virulence2008

    • Author(s)
      Ichinose, Y., et. al.
    • Journal Title

      In Pseudomonas syringae Pathovars and Related Pathogens-Identification, Epidemiology and Genomics (M. Fatmi, et. al. eds.), Springer

      Pages: 167-173

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Modulation of defense signal transduction by flagellin-induced WRKY41 transcription factor in Arabidopsis thaliana2008

    • Author(s)
      Higashi, K., et. al.
    • Journal Title

      Mol Genet. Genomics 279(3)

      Pages: 303-312

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Gac two-component system in Pseudomonas syringae pv. tabaci is required for virulence but not for hypersensitive reaction2008

    • Author(s)
      Marutani, M., et. al.
    • Journal Title

      Mol. Genet. Genomics 279(4)

      Pages: 313-322

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The model plant Medicago truncatula exhibits biparental plastid inheritance2008

    • Author(s)
      Matsushima, R., et. al.
    • Journal Title

      Plant Cell Physiol 49(1)

      Pages: 81-91

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Ultrastructural features of Mycosphaerella pinodes Infection and differenced in host susceptibility responses among Medicago truncatula ecotypes2007

    • Author(s)
      Suzuki, T., et. al.
    • Journal Title

      J. Electr. Microsc. Technol. Med. Biol. 20

      Pages: 167-168

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Elicitin-responsive lectin-like receptor kinase genes in BY-2 cells2007

    • Author(s)
      Sasabe, et. al.
    • Journal Title

      DNA Sequence 18

      Pages: 152-159

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Suppression of Cdc27B expression induces plant defence responses2007

    • Author(s)
      Kudo, et. al.
    • Journal Title

      Mol. Plant Pathol. 8

      Pages: 365-373

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] N-terminal domain including conserved flg22 is required for flagellin-induced hypersensitive cell death in Arabidopsis thaliana2007

    • Author(s)
      Naito, et. al.
    • Journal Title

      J. Gen. Plant Pathol. 73

      Pages: 281-285

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Flagellin glycans from two pathovars of Pseudomonas syringae contain rhamnose in D and L configurations in different ratios and modified 4-amino-4, 6-dideoxyglucose2007

    • Author(s)
      Takeuchi, et. al.
    • Journal Title

      J. Bacteriol. 189

      Pages: 6945-6956

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Saccharomyces cerevisiae SSD1 orthologues are essential for host infection by the ascomycete plant pathogens Colletotrichum lagenarium and Magnapothe grisea2007

    • Author(s)
      Tanaka, et. al.
    • Journal Title

      Molecular Microbiology 64

      Pages: 1332-1349

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Elicitin-responsive lectin-like receptor kinase genes in BY-2 cells2007

    • Author(s)
      Sasabe, M., et. al.
    • Journal Title

      DNA Sequence 18(2)

      Pages: 152-159

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Ultrastructural features of Mycosphaerella pinodes Infection and differenced in host susceptibility responses among Medicago truncatula Ecotypes2007

    • Author(s)
      Suzuki, T., et. al.
    • Journal Title

      J. Electr. Microsc. TechnoL Med. Biol 20(2)

      Pages: 167-168

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Suppression of Cdc27B expression induces plant defence responses2007

    • Author(s)
      Kudo, C., et. al.
    • Journal Title

      Mol. Plant Pathol 8(4)

      Pages: 365-373

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] N-terminal domain including conserved flg22 is required for flagellin-induced hypersensitive cell death in Arabidopsis thaliana2007

    • Author(s)
      Naito, K., et. al.
    • Journal Title

      J. Gen. Plant Pathol 73(4)

      Pages: 281-285

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Flagellin glycans from two pathovars of Pseudomonas syringae contain rhamnose in D and L configurations in different ratios and modified 4-amino-4,6-dideoxyglucose2007

    • Author(s)
      Takeuchi, K., et. al.
    • Journal Title

      J. Bacteriol 189(19)

      Pages: 6945-6956

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Saccharomyces cerevisiae SSD1 orthologues are essential for host infection by the ascomycete plant pathogens Colletotrichum lagenarium and Magnaporthe grisea2007

    • Author(s)
      Tanaka, S., et. al.
    • Journal Title

      Molecular Microbiology 64

      Pages: 1332-1349

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Activation of plant immune system and its regulation by flagellin from Pseudomonas syringae2007

    • Author(s)
      Ichinose, Y., et. al.
    • Journal Title

      In Virulence of microbe and plant defense response, Hokkaido University Shuppannkai, Sapporo, (I. Uyeda ed.)

      Pages: 132-143

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mechanism of MAMP signal transduction2007

    • Author(s)
      Ichinose, Y
    • Journal Title

      Protein, Nucleic acid and Enzyme 52(6)

      Pages: 635-641

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] What is an assignment of physiological plant pathology today?-Opinion of an old phytopathologist walking at the wayside2007

    • Author(s)
      Shiraishi, T
    • Journal Title

      In Dynamism of Plant-Pathogen Interactions (eds. by Y. Kubo, et. al.), Phytopathological Society of Japan (Tokyo)

      Pages: 141-150

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The important role of plant cell wall in signal perception and defense response2007

    • Author(s)
      Shiraishi, T
    • Journal Title

      In Virulence of microbe and plant defense response, Hokkaido University Shuppannkai, Sapporo, (I. Uyeda ed.)

      Pages: 10-21

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] イネにおけるサポニン生合成経路についての分子解析2008

    • Author(s)
      稲垣善茂、A. OSBOURN
    • Organizer
      平成20年度日本植物生理学会
    • Place of Presentation
      札幌市
    • Year and Date
      20080320-22
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Molecular analyses for saponin biosynthesis pathway in rice2008

    • Author(s)
      Inagaki, Y., Osbum, A
    • Organizer
      The 2008 Annual Meeting of the Japanese Society of Plant Physiologists
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      20080320-22
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] イネにおけるOxidosqualene Cyclase遺伝子群のゲノム構造と遺伝子発現2007

    • Author(s)
      稲垣善茂, ら
    • Organizer
      平成19年日本遺伝学会
    • Place of Presentation
      岡山市
    • Year and Date
      20071019-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Molecular analysis for genome structure and gene expression of Oxidosqualene Cyclase genes in rice seedling2007

    • Author(s)
      Inagaki, Y., et. al.
    • Organizer
      The 79th Annual Meeting of the Genetics Society of Japan
    • Place of Presentation
      Okayama, Japan
    • Year and Date
      20071019-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] タバコ野火病菌べん毛タンパク質フラジェリンの防御応答誘導活性とべん毛の機能解析2007

    • Author(s)
      内藤佳奈, 他
    • Organizer
      平成19年度日本植物病理学会関西部会
    • Place of Presentation
      岐阜市
    • Year and Date
      20071006-07
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] エフェクター分泌と感染の成否との間2007

    • Author(s)
      白石友紀
    • Organizer
      平成19年度日本植物病理学会関西部会
    • Place of Presentation
      岐阜市
    • Year and Date
      20071006-07
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] イネにおけるサポニン生合成経路遺伝子Oxidosqualene Cyclaseの分子解析2007

    • Author(s)
      稲垣善茂、A. OSBOURN
    • Organizer
      平成19年度日本植物病理学会関西部会
    • Place of Presentation
      岐阜市
    • Year and Date
      20071006-07
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Functional analysis of flagellar motility and elicitor activity of flagellar protein flagellin in Pseudomonas syringae pv. tabaci2007

    • Author(s)
      Naito, et. al.
    • Organizer
      The meeting of the Kansai division, Phytopathological Society of Japan
    • Year and Date
      20071006-07
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Effectors and their significance in plant-microbe interactions2007

    • Author(s)
      Shiraishi, T
    • Organizer
      The Meeting of the Kansai division, Phytopathological Society of Japan
    • Place of Presentation
      Gifu
    • Year and Date
      20071006-07
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Molecular analysis for Oxidosqualene Cyclase in saponin biosynthesis pathway of rice2007

    • Author(s)
      Inagaki, Y., Osburn, A
    • Organizer
      The 2007 Kansai-Branch Meeting of the Phytopathological Society of Japan
    • Place of Presentation
      Gifu, Japan
    • Year and Date
      20071006-07
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] シロイヌナズナによるタバコ野火病菌べん毛タンパク質フラジェリンの認識2007

    • Author(s)
      内藤佳奈, 他
    • Organizer
      日本植物病理学会平成19年度植物感染生理談話会
    • Place of Presentation
      京都市
    • Year and Date
      20070809-11
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 今、感染生理学は何を明らかにすべきか?路傍から2007

    • Author(s)
      白石友紀
    • Organizer
      日本植物病理学会平成19年度植物感染生理談話会
    • Place of Presentation
      京都市
    • Year and Date
      20070809-11
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Recognition of Pseudomonas syringae pv. tabaci flagellin in Arabidopsis thaliana2007

    • Author(s)
      Naito, et. al.
    • Organizer
      Dyna,ism of Plant-Pathogen Interactions (43th PSJ Plant-Microbe Interactions Symposium)
    • Place of Presentation
      Kyoto
    • Year and Date
      20070809-11
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] What is an assignment of physiological plant pathology today?-Opinion of an old phytopathologist walking at the wayside2007

    • Author(s)
      Shiraishi, T
    • Organizer
      43rd PSJ Plant-Microbe Interactions Symposium
    • Place of Presentation
      Kyoto
    • Year and Date
      20070809-11
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Pseudomonas syringaeのMAMPsと植物防御応答の回避機構2007

    • Author(s)
      一瀬勇規
    • Organizer
      玉田シンポジウム(第21回岡山植物病理セミナー)
    • Place of Presentation
      倉敷市
    • Year and Date
      2007-04-21
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] MAMPs in Pseudomonas syringae and bacterial strategy to avoid plant defense response2007

    • Author(s)
      Yuki, Ichinose
    • Organizer
      The 1st Tamada Symposium (21st Seminar of Plant Pathology in) Kurashiki
    • Place of Presentation
      Okayama
    • Year and Date
      2007-04-21
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Pseudomonas syringae Pathovars and Related Pathogens-Identification, Epidemiology and Genomics(M. Fatmi, et. al. eds.)pp.167-1732008

    • Author(s)
      Ichinose, Y., et. al.
    • Total Pages
      7
    • Publisher
      Springer-Verlag
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 植物-病原微生物の相互作用のダイナミズム(久保康之ら編)pp.141-1502007

    • Author(s)
      白石友紀
    • Total Pages
      10
    • Publisher
      日本植物病理学会(東京)
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 微生物の病原性と植物の防御応答(上田一郎編著)pp.10-212007

    • Author(s)
      白石友紀
    • Total Pages
      12
    • Publisher
      北海道大学出版会(札幌)
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 微生物の病原性と植物の防御応答(上田一郎編著)pp.132-1432007

    • Author(s)
      一瀬勇規
    • Total Pages
      12
    • Publisher
      北海道大学出版会(札幌)
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 蛋白質 核酸 酵素 増刊号「植物における環境と生物ストレスに対する応答pp.635-6412007

    • Author(s)
      一瀬勇規
    • Total Pages
      7
    • Publisher
      共立出版
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-02-04  

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