2007 Fiscal Year Final Research Report Summary
Single molecule analysis of chemotactic signaling system
| Project/Area Number |
15109003
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | Osaka University |
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Principal Investigator |
YANAGIDA Toshio Osaka University, Graduate School ofFrntierBiosiences, Professor (30089883)
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| Co-Investigator(Kenkyū-buntansha) |
SAKO Yasushi RIKEN, Cellular Infonnatics Laboratory, Chief Scientist (20215700)
IWANE Atsuko Osaka University, Graduate School of Frontier Biosienoes, Associate Professor (30252638)
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| Project Period (FY) |
2003 – 2007
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| Keywords | Chemotaxis / Single molecule / Dietvostelium discoideum / Neuron / Fluctuations / Signal transduction / GPCR / NGF |
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| Research Abstract |
(300 words)
Chemotaxis, the process by which cells sense and respond directionally to chemical gradients, operates in a wide range of biological processes including immunity, neuronal patterning, and morphogenesis. How chemotactic cells reliably obtain information regarding the gradient from such noisy inputs is a critical question for directional sensing in chemotaxis.
In this research, we have developed single molecule imaging techniques to monitor directly behaviors of individual bio-molecules in chemotactic signaling system. Single molecule imaging analysis of chemotactic response in eukaryotic cells revealed a stochastic nature in the input signals and the signal transduction processes. Also, we have developed a stochastic model of chemotactic signaling in which noise and signal propagation along transmembrane signaling by chemoattractant receptors can be analyzed quantitatively. The results obtained from these analysis reveal that the second messenger production reactions by the r
… More
eceptors generate noisy signals, which contain intrinsic noise inherently generated at this reaction and extrinsic noise propagated from the ligand-receptor-binding. Such intrinsic and extrinsic noises limit directional sensing ability of chemotactic cells which can explain the dependence of chemotactic accuracy on chemical gradients that have been observed experimentally. Our analysis also reveals regulatory mechanism for signal improvements in the stochastically-operating signaling system by analyzing how signal-to-noise ratio (SNR) of chemotactic singals can be improved or deteriorated by the stochastic properties of receptors and second messenger molecules.
Our model provides a theoretical framework with experimental approaches to chemotactic signaling system and can further be applied to other stochastic signaling systems in general. Furthermore, inspired by biological processing, in which organisms manage successfully to acquire noise-robust characteristics and flexibility in their information processing, we proposed a stochastic calculation method based on the concept of stochastic computing. This will provide a complementary approach to technology based on conventional computing principles. Less
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[Journal Article] Biologically-inspired stochastic vector matching for noise-robust information processing2008
Author(s)
Ueda, M., Ueda, M., Takagi, H., Sato, M. J, Yanagida, T, Yamashita, I., Setsune, K
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Single-molecule imaging of fluorescent proteins expressed in living cells2008
Author(s)
Hibino, IC, Hiroshima, K, Takahashi, M., Sako, Y
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Journal Title
(Lee, J, W. ed. Humana Press.), Methods Mol. Biol (in press)
Description
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[Journal Article] Input-output relationship in galvanotactic response of Dictyostelium cells!2007
Author(s)
Sato, M. J., Ueda, K, Takagi, H., Watanabe, T. M., Yanagida, T., Ueda, M
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Journal Title
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[Journal Article] Role of Multiple Bonds Between the Single Cell Adhesion Molecules, Nectin and Cadherin, Revealed by High Sensitive Force Measurements2007
Author(s)
Tsukasaki,Y., Kitamura, K, Shimizu, K, Iwane, A. H., Takai, Y., Yanagida, T
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Journal Title
J. Mol. Biol 357
Pages: 996-1006
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Multiple-state reactions between the epidermal growth factor receptor and Grb2 as observed using single-molecule analysis2007
Author(s)
Morimatsu, K, Takagi, H., Ota, K G., Iwamoto, R., Yanagida, T., Sako, Y
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Journal Title
Proc. Nati Acad. Sd. USA 104
Pages: 18013-18018
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Brownian motion, fluctuation and life2007
Author(s)
Yanagida, T., Ueda, M., Murata, T., Esaki, S., Ishii, Y
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Journal Title
Biosystems 88
Pages: 228-242
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Tumor suppressor PTEN acts through dynamic interaction with the plasma membrane2006
Author(s)
Vazquez, F., Matsuoka, S., Sellers, W. R, Yanagida, T., Ueda, M., Devreotes, P
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Journal Title
Proc. Nati Acad. SM. USA 103
Pages: 3633-3638
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Single molecule analysis of chemoattractant-stimulated membrane recruitment of a PH domain-containing protein2006
Author(s)
Matsuoka, S., Iijima, M., Watanabe, T.M., Kuwayama, H., Yanagida, T., Devreotes, P., Ueda M
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Journal Title
J. Cell Sd 119
Pages: 1071-1079
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Single-molecule analysis of epidermal growth factor binding on the surface of living cells2006
Author(s)
Teramura, Y., Ichinose, J., Takagi, H., Nishida, K, Yanagida, T., Sako, Y
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Journal Title
EMBO J 25
Pages: 4215-4222
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Dynamic polymorphism of Has observed by single molecule FRET is the basis for molecular recognition2006
Author(s)
Arai, Y, Iwane, AH., Wazawa, T., Yokota, H., Ishii, Y., Kataoka, T., Yanagida, T
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Journal Title
Biochem. Biophys. Res. Comm 343
Pages: 809-815
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Trafficking of a Ligand-Receptor Complex on the Growth Cones as an Essential Step for the Uptake of Nerve Growth Factor at the Distal End of the Axon : A Single-Molecule Analysis2005
Author(s)
Tani, T., Miyamoto, Y., Fujimori, K., Taguchi, T., Yanagida, T., Sako, Y, Harada, Y
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Journal Title
Neurosci 25
Pages: 2181-2191
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Single- and multiple-molecule dynamics of the signaling from H-Ras to cltaf-1 visualized on the plasma membrane of living cells2003
Author(s)
Hibino, K, Watanabe, T.M., Kozuka, J., Iwane, A.H., Okada, T., Kataoka, T., Yanagida, T., Sako Y
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Journal Title
CHEM PHYS CHEM4
Pages: 748-753
Description
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[Journal Article] Engagement of CD44 Promotes Rac Activation and CD44 Cleavage during Tumor Cell Migration2003
Author(s)
Murai, T., Miyazaki, Y, Nishinakamura, H., Sugahara, K.N., Miyauchi, T., Sako, Y., Yanagida, T., Miyasaka, M
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Journal Title
J Biol. Chem 279
Pages: 4541-4550
Description
「研究成果報告書概要(欧文)」より
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