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2005 Fiscal Year Final Research Report Summary

Development and application of highly integrated protein and cell chip devices

Research Project

Project/Area Number 15201032
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Microdevices/Nanodevices
Research InstitutionJapan Advanced Institute of Science and Technology

Principal Investigator

TAMIYA Eiichi  Japan Advanced Institute of Science and Technology, School of Materials Science, Professor, 材料科学研究科, 教授 (60179893)

Co-Investigator(Kenkyū-buntansha) TAMKAMURA Yuzuru  Japan Advanced Institute of Science and Technology, School of Materials Science, Associate Professor, 材料科学研究科, 助教授 (20290877)
NAKANO Hideo  Nagoya university, Graduate School of Bioagricultural Sciences, Professor, 大学院・生命農学研究科, 教授 (00237348)
KISHI Hiroyuki  University of Toyama, Faculty of Medicine, Associate Professor, 医学部, 助教授 (60186210)
Project Period (FY) 2003 – 2005
KeywordsBiochip / DNA chip / Cell chip / Single-cell analysis / Antibody / Drug screening / PCR / Micro-, nanotechnology
Research Abstract

Though the number of human genes was reported to be approximately 30,000 from human genomic project, the functions and expression mechanism for most of the genes are remain to be unknown. Because the fate of the genes functionality is determined after protein expression, but proteins expression is controlled by cellular function. Therefore, it is necessary to develop the microarray chip devices that can perform high-throughput screening and analysis of proteins and cells at single-cell and single-molecule level. For achieving single-cell or single-molecule analysis, highly integrated microarray systems that can perform assays at pico- and nano-liter volume level are greatly desirable to realize post-genomic research, such as proteomics and cellomics. In our project research, for achieving simultaneous detection of several numbers of target DNA, the feasibility of our microchamber array was improved by using TaqMan PCR. Three different DNA sequences were amplified from three different D … More NA templates and detected in the same microchamber array simultaneously. In addition, the quantification of initial DNA concentration present in a microchamber was achieved from 0 to 12 copies per chamber, not only by monitoring the real-time fluorescence intensity but also by observing the end point fluorescence signal. Therefore, this system proves to be a promising device for the low-cost, high-throughput DNA amplification and detection for point-of-care clinical diagnosis, which can also be handled by non-specialist users.
Further, we report improved microchamber array to monitor Ca^<2+> mobilization of over 25,000 cells simultaneously at a single-cell level. And we have developed a novel high-throughput screening and analysis system for antigen-specific single B-cells using the microarray, which was carried out by detecting antigen-specific single B-cells against an antigen of interest. The single-cell microarray system does not need to use myeloma as in the case of conventional hybridoma technique, and can screen the antigen-specific single B-cells directly from cell suspension and analyze antigen-specific antibody DNA at a single-cell level. This system is simple and easy in its operation, and quick enough for making monoclonal antibodies when compared to conventional techniques. Moreover this system can perform high-throughput single-cells analysis using chip devices.
Therefore, we have addressed the analysis of DNA, protein and cell using pico- or nano-liter chamber array system in this project. They might also be applicable for detection of DNA and cells, which lead to immune therapy or gene therapy in the future. Less

  • Research Products

    (25 results)

All 2005 2004

All Journal Article (23 results) Book (2 results)

  • [Journal Article] Single-cell microarray for analysing cellular response.2005

    • Author(s)
      S.Yamamura et al.
    • Journal Title

      Analytical Chemistry 77

      Pages: 8050-8056

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Localized surface plasmon resonance based optical biosensor using surface modified nanoparticle layer for label-free monitoring antigen-antibody reaction.2005

    • Author(s)
      T.Endo et al.
    • Journal Title

      Science and Technology of Advanced Materials 6

      Pages: 491-500

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Construction of novel single cell screening system using a yeast cell chip for nano-sized modified-protein-dispensing libraries.2005

    • Author(s)
      T.Fukuda et al.
    • Journal Title

      Naobiotechnology 1

      Pages: 105-111

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Fluorescence-based assay with enzyme amplification on a micro-flow immunosensor chip for monitoring coplanar polychlorinated biphenyls.2005

    • Author(s)
      T.Endo et al.
    • Journal Title

      Analytical Chimical Acta 531

      Pages: 7-13

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Microfabrication of encoded microparticle array for multiplexed DNA hybridization detection.2005

    • Author(s)
      Z.L Zhi et al.
    • Journal Title

      Chemical Communication 19

      Pages: 2448-2450

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Label-free detection of peptide nucleic acid-DNA hybridization using localized surface plasmon resonance based optical biosensor.2005

    • Author(s)
      T.Endo et al.
    • Journal Title

      Analytical Chemistry 77

      Pages: 6976-6984

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Microchamber array based DNA quantification and specific sequence detection from a single copy via PCR in nanoliter volumes2005

    • Author(s)
      Y.Matsubara et al.
    • Journal Title

      Biosensors and Bioelectronics 20(8)

      Pages: 1482-1490

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Single-cell microarray for analysing cellular response.2005

    • Author(s)
      S.Yamamura et al.
    • Journal Title

      Anal.Chem. 77

      Pages: 8050-8056

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Localized surface plasmon resonance based optical biosensor using surface modified nanoparticle layer for label-free monitoring antigen-antibody reaction.2005

    • Author(s)
      T.Endo et al.
    • Journal Title

      Sci.Technol.Adv.Mater. 6

      Pages: 491-500

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Fluorescence-based assay with enzyme amplification on a micro-flow immunosensor chip for monitoring coplanar polychlorinated biphenyls.2005

    • Author(s)
      T.Endo et al.
    • Journal Title

      Anal.Chim.Act. 531

      Pages: 7-13

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Microfabrication of encoded microparticle array for multiplexed DNA hybridization detection.2005

    • Author(s)
      Z.L.Zhi et al.
    • Journal Title

      Chem.Commun. 19

      Pages: 2448-2450

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Label-free detection of peptide nucleic acid-DNA hybridization using localized surface plasmon resonance based optical biosensor.2005

    • Author(s)
      T.Endo et al.
    • Journal Title

      Anal.Chem. 77

      Pages: 6976-6984

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Microchamber array based DNA quantification and specific sequence detection from a single copy via PCR in nanoliter volumes.2005

    • Author(s)
      Y.Matsubara et al.
    • Journal Title

      Biosens Bioelectron. 20

      Pages: 1482-1490

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] On-chip DNA quantification and specific sequence detection from a single copy by counting fluorescence released microchambers via PCR in nanoliter volumes2004

    • Author(s)
      Y.Matsubara et al.
    • Journal Title

      Analytical Chemistry 334

      Pages: 111-116

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A picoliter Chamber Array for Cell-Free Protein Synthesis2004

    • Author(s)
      T.Kinpara et al.
    • Journal Title

      J.Biochemistry 136

      Pages: 149-154

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Electrochemical coding of single nucleotide polymorphisms by monobase modified gold nanoparticles,2004

    • Author(s)
      K.Kerman et al.
    • Journal Title

      Analytical Chemistry 76

      Pages: 1877-1884

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] On-chip micro-flow polystyrene bead-based immunoassay for quantitative detection of tacrolimus2004

    • Author(s)
      Y.Murakami et al.
    • Journal Title

      Analytical Biochemisty 334

      Pages: 111-116

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Peptide nucleic acid modified magnetic beads for intercalator based electrochemical detection of DNA hybridization2004

    • Author(s)
      K.Kerman et al.
    • Journal Title

      Science and Technology of Advancecd Materials 5(3)

      Pages: 351-357

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] On-chip nanoliter-volume multiplex TaqMan polymerase chain reaction from a single copy based on counting fluorescence released microchambers.2004

    • Author(s)
      Y.Matsubara et al.
    • Journal Title

      Anal Chem. 76

      Pages: 6434-6439

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A picoliter chamber array for cell-free protein synthesis.2004

    • Author(s)
      T.Kinpara et al.
    • Journal Title

      J.Biochem. (Tokyo) 136

      Pages: 149-154

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Electrochemical coding of single nucleotide polymorphisms by monobase modified gold nanoparticles.2004

    • Author(s)
      K.Kerman et al.
    • Journal Title

      Anal.Chem. 74

      Pages: 1877-1884

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] On-chip micro-flow polystyrene bead-based immunoassay for quantitative detection of tacrolimus (FK506).2004

    • Author(s)
      Y.Murakami et al.
    • Journal Title

      Anal Biochem. 334

      Pages: 111-116

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Peptide nucleic acid modified magnetic beads for intercalator based electrochemical detection of DNA hybridization.2004

    • Author(s)
      K.Kerman et al.
    • Journal Title

      Sci.Technol.Adv.Mater. 5

      Pages: 351-357

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 医療用マテリアルと機能膜2005

    • Author(s)
      民谷栄一ら
    • Total Pages
      233-247
    • Publisher
      シーエムシー出版
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Protein Nanotechnology2005

    • Author(s)
      E.Tamiya et al.
    • Total Pages
      452
    • Publisher
      Humana Press
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2007-12-13  

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