2006 Fiscal Year Final Research Report Summary
Mechanism of photoactivation of photoactivated adenylyl cyclase (PAC), a novel blue-light receptor, and mechanism of photocontrol of cellular functions.
| Project/Area Number |
15207006
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
植物生理・分子
|
|---|
| Research Institution | The Graduate University for Advanced Studies (2004-2006)
Okazaki National Research Institutes (2003) |
|---|
Principal Investigator |
WATANABE Masakatsu The Graduate University for Advanced Studies, School of Advanced Sciences, Professor, 先導科学研究科, 教授 (40124226)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KANDORI Hideki Nagoya Institute for Technology, School of Engeneering Sciences, Professor, 工学研究科, 教授 (70202033)
|
|---|
| Project Period (FY) |
2003 – 2006
|
| Keywords | blue-light sensor / photoactivated enzyme / cAMP / Euglena / photobehavior / PAC / flavin |
|---|
| Research Abstract |
Photoactivated adenylyl cyclase (PAC) (Iseki et al. 2002 Nature) is a blue-light sensor protein responsible for photoavoidance behavior in a eukaryotic unicellular flagellate alga Euglena gracilis. It is composed of flavin-binding, light sensing domains and adenylyl cyclase catalytic domains so that it produces cAMP, a versatile intracellular second messenger, in response to blue-light irradiation.
By molecular phylogenetic analyses of homologues fo PAC in various euglenoids, it was suggested that PAC was introduced into Euglena almost concomitantly with the secondary symbiotic introduction of its chroloplasts (Koumura et al. 2004 Photochem Photobiol Sci).
By analyses of the photoactivation kinetics of purified PAC from Euglena, action spectrum and responsively to repeated short pulse irradiations were determined as well as intracellular cAMP concentration time courses. These results were in accordance with the corresponding in vivo photobehavior characteristics (Yoshikawa et al. 2005 Ph
… More
otochem Photobiol Sci).
By analyses of the photochemical cycle kinetics of a flavin-binding domain heterologously expressed in E. coil, threshold light flounce rate, quantum efficiency as well as half life of dark recovery ware determined which were in general similarity to other members of the so-called BLUF family of flavin-binding domains, mostly of prokaryotic origins (Ito et al. 2005 Photochem Photobiol Sci).
The unique distinguishing feature of PAC to act as a light sensor having intrinsic effector function of adenylyl cyclase provide promising possibilities to be used as a tool to photomanipulate various cAMP-dependent biological functions including neural and reproductive processes if it is successfully expressed functionally in heterologous systems (Iseki et al. 2002 Nature). This possibility was in fact realized by successful functional expression of PAC in frog oocytes, human cultured cells as well as in fly brains, particularly in which grooming behavior of the fly individual was specifically photosuppressed by external light irradiation (Schroeder-Lang 2007 Nature Methods). Less
|
-
-
[Journal Article] Fast manipulation of cellular cAMP level by light in vivo.2007
Author(s)
Schroder-Lang, S., Schwarzel, M., Seifert, R., Strunker, T., Kateriya, S., Looser, J., Watanabe, M., Kaupp, U.B., Hegemann, P., Nagel, G.
-
Journal Title
Nat.Methods. 4 (1)
Pages: 39-42
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
[Journal Article] Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of cyanobacteria.2005
Author(s)
Okajima, K., Yoshihara, S., Fukushima, Y., Geng, X., Katayama, M., Higashi, S., Watanabe, M., Sato, S., Tabata, S., Shibata, Y., Itoh, S., Ikeuchi, M.
-
Journal Title
J.Biochem. 137 (6)
Pages: 441-450
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Photocycle features of heterologously expressed and assembled eukaryotic flavin-binding BLUF domains of photoactivated adenylyl cyclase (PAC), a blue-light receptor in Euglena gracilis.2005
Author(s)
Ito, S., Murakami, A., Sato, K., Nishina, Y., Shiga, K., Takahashi, T., Higashi, S., Iseki, M., Watanabe, M.
-
Journal Title
Photochem.Photobiol.Sci. 4 (9)
Pages: 762-769
Description
「研究成果報告書概要(欧文)」より
-
-
[Journal Article] The origin of photoactivated adenylyl cyclase (PAC), the Euglena blue-light receptor : phylogenetic analysis of orthologues of PAC subunits from several euglenoids and trypanosome-type adenylyl cyclases from Euglena gracilis.2004
Author(s)
Koumura, Y., Suzuki, T., Yoshikawa, S., Watanabe, M., Iseki, M.
-
Journal Title
Photochem.Photobiol.Sci. 3 (6)
Pages: 580-586
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
