2005 Fiscal Year Final Research Report Summary
Regulation of the synthesis and release of pituitary hormones by novel hypothalamic neurohormones
| Project/Area Number |
15207007
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Hiroshima University |
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Principal Investigator |
TSUTSUI Kazuyoshi Hiroshima University, Graduate School of Integrate Arts and Sciences, Professor, 大学院総合科学研究科, 教授 (20163842)
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| Co-Investigator(Kenkyū-buntansha) |
KIKUYAMA Sakae Waseda University, Faculty of Education, Professor, 教育総合科学学術院, 教授 (20063638)
MINAKATA Hiroyuki Suntory Institute for Bioorganic Research, General Manager, 部長研究員 (90150143)
UKENA Kazuyoshi Graduate School of Integrate Arts and Sciences, Associate Professor, 大学院総合科学研究科, 助教授 (10304370)
TAKAHASHI Toshio Suntory Institute for Bioorganic Research, Researcher, 研究員 (20390792)
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| Project Period (FY) |
2003 – 2005
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| Keywords | hypothalamic neurohormones / GnIH / GnIH homologOus peptides / GnIH receptor / pituitary hormones / gonadotropin / prolactin / reproduction |
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| Research Abstract |
Probing undiscovered neuropeptides that play important roles in the regulation of pituitary function in vertebrates is essential for the progress of neuroendocrinology. In this research, We identified a novel hypothalamic neuropeptide with a C-terminal LPLRF-amide sequence in the quail brain. This avian neuropeptide was, shown to be located in the hypothalamo-hypophysial system and to decrease gonadotropin release. We therefore designated this novel neuropeptide as gonadotropin-inhibitory hormone (GnIH). To elucidate the mode of action of GnIH, we then' identified a novel G protein:-coupled receptor for GnIH in quail. The GnIH receptor possesses seven transmembrane domains and specifically binds to GnIH. The GnIH receptor is expressed in the pituitary and several brain regions including the hypothalamus. Thus, GnIH acts directly on the pituitary via GnIH receptor to inhibit gonadotropin release. To demonstrate the functional significance of GnIH and its potential role as a key regulatory neuropeptide in avian reproduction, we investigated GnIH actions on gonadal development and maintenance in quail. Chronic treatment with GnIH inhibited gonadal development and maintenance by decreasing gonadotropin synthesis and release. GnIH was also found in the hypothalamus of other avian species including sparrows and chickens and also inhibited gonadotropin synthesis and release. Subsequently, we further identified novel hypothalamic neuropeptides closely related to GnIH in the brains of other vertebrates, such as mammals, amphibians, and fish. The identified neuropeptides possessed a LPXRF-amide (X = L or Q) motif at 'their C-termini. These GnIH homologous peptides (LPXRF-amide peptides) also were localized in the hypothalamus and other brainstem areas and regulated the release of gonadotropins and prolactin. Thus, GnIH and GnIH homologous peptides are key factors controlling vertebrate reproduction.
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[Journal Article] Civelli Salusin (beta) is a surrogate ligand of the mas-like G protein-coupled receptor MrgAl.2006
Author(s)
Z.Wang, T.Takahashi, Y.Saito, H.Nagasaki, N.K.Ly, H.-P.Nothacker, R.K.Reinscheid, J.Yang, J.K.Chang, M.Shichiri, 0.
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Journal Title
Eur. J. Pharmacol. 539
Pages: 145-150
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Mode of action and functional significance of avian gonadotropin-inhibitory hormone (GnIH) : A review.2006
Author(s)
K.Tsutsui, T.Ubuka, H.Yin, T.Osugi, K.Ukena, G E.Bentley, N.Ciccone, K.Inoue, V.S.Chowdhury, P.J.Sharp, J.C.Wingfield
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Journal Title
J. Exp. Zool. 305A
Pages: 801-806
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Hym-301, a novel peptide, regulates the number of tentacles formed in hydra.2005
Author(s)
T.Takahashi, M.Hatta, S.Yum, 0.Koizumi, Y.Kobayakawa, L.Gee, M.Ohtani, T.Fujisawa, H.R.Bode
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Journal Title
Development 132
Pages: 2225-2234
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Hormonal regulation of growth in amphibians.2005
Author(s)
S.Kikuyama, K.Yamamoto, T.Kobayashi, M.-C.Tonon, L.Galas, H.Vaudry
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Journal Title
Biology of Amphibians vol.6 : Endocrinology of Amphibians (H. Heatwale (ed)) (Surrey Beatty and Sons, Chipping Norton, Australia)
Pages: 2267-2300
Description
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[Journal Article] Tachykinin and tachykinin receptor of an ascidian, Ciona intestinalis : evolutionary origin of the vertebrate tachykinin family.2004
Author(s)
H.Satake, M.Ogasawara, T.Kawada, K.Masuda, M.Aoyama, H.Minakata, T.Chiba, H.Metoki, Y.Satou, N.Satoh
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Journal Title
J. Biol. Chem. 279
Pages: 53798-53805
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effect of pituitary adenylate cyclase-activating polypeptide 38 (PACAP38) on tissue oxygen content-Treatment in central nervous system of mice.2004
Author(s)
H.Ohtaki, K.Dohi, S.Yofu, T.Nakamachi, Y.Kudo, S.Endo, T.Aruga, N.Goto, J.Watanabe, S.Kikuyama, S.Shioda
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Journal Title
Regul. Pept. 123
Pages: 61-67
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Comparative aspects of novel hypothalamic LPXRF-amide peptides in the vertebrate brain : Structure, localization and function.2004
Author(s)
K.Ukena, K.Sawada, A.Kod, K.Yamamoto, S.Kikuyama, H.Satake, E.Iwakoshi-Ukena, H.Minakata, K.Tsutsui
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Journal Title
Trends in Comparative Endocrinology (T. Oishi, K. Tsutsui, S. Tanaka and S. Kikuyama (eds)) (Asia and Oceania Society for Comparative Endocrinology)
Pages: 325-327
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] PRQFVamide, a novel pentapeptide identified from the CNS and gut of Aplysia.2003
Author(s)
Y.Furukawa, K.Nakamaru, K.Sasaki, Y.Fujisawa, H.Minakata, S.Ohta, F.Morishita, 0.Matsushima, L.Li, V.Alexeeva, T.A.Ellis, N.C.Dembrow, J.Jing, J.V.Sweedler, K.R.Weiss, F.S.Vilim
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Journal Title
J. Neurophysiol. 89
Pages: 3114-3127
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of a new member of the LWamide peptide family.2003
Author(s)
T.Takahashi, Y.Kobayakawa, Y.Muneoka, Y.Fujisawa, S.Mohri, M.Hatta, H.Shimizu, T.Fujisawa, T.Sugiyama, M.Takahara, K.Yanagi, 0.Koizumi
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Journal Title
Comp. Biochem. Physiol. Part B 135
Pages: 309-324
Description
「研究成果報告書概要(欧文)」より
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