2005 Fiscal Year Final Research Report Summary
Sorting and membrane localization of E.coli lipoproteins
| Project/Area Number |
15208009
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | University of Tokyo |
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Principal Investigator |
TOKUDA Hajime University of Tokyo, Institute of Molecular and Cellular Biosciences, Professor, 分子細胞生物学研究所, 教授 (40125943)
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| Project Period (FY) |
2003 – 2005
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| Keywords | protein transport / molecular chaperone / reconstitution / Escherichia coli / ABC transporter / lipoprotein / membrane localization / sorting signal |
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| Research Abstract |
E.coli lipoproteins are sorted to the outer membrane depending on the amino acid residue located next to the amino-terminal lipid-attached cysteine. The sorting is mediated by the Lol system composed of five Lol proteins. The lipoprotein transfer reaction from LolA to LolB is unidirectional and requires no energy. This reaction was found to depend on the strength of hydrophobic interaction between lipoproteins and LolA or LolB. If the LolA-lipoprotein interaction is too strong, the transfer of lipoprotein to LolB is inhibited. Opening and closing of the hydrophobic cavity of LolA was found to be critical for the LolA function. Finally, the region of LolD important for the interaction with membrane subunits LolC and LolE was determined.
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