• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2005 Fiscal Year Final Research Report Summary

Development of mesenchymal stem cell engineering for reconstructive therapy

Research Project

Project/Area Number 15208034
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionNagoya University

Principal Investigator

KITAGAWA Yasuo  Nagoya Univ., Grad. Sch. Bioagric. Sci., Professor, 大学院・生命農学研究科, 教授 (50101168)

Co-Investigator(Kenkyū-buntansha) TORII Shuhei  Nagoya Univ., Grad. Sch. Medicine., Professor, 大学院・医学系研究科, 教授 (60115607)
Project Period (FY) 2003 – 2005
Keywordsadipose tissues / age-dependency / autologous stem cells / fibroblast growth factor / humanized culture / low-serum medium / mesenchym / stromal vascular fraction
Research Abstract

Adipose tissues are abundant deposition sites of autologous stem cells with potential application for regenerative medicine and adipose-derived stromal vascular fraction (SVF) is a source of autologous stem cells with differentiation potential along mesenchymal, hematopoietic, neurogenic, angiogenic or epithelial lineage. By culturing SVF cells in high- or low- serum medium containing fibroblast growth factor (FGF)-2, we found that the cells grew faster in low-serum medium and could be subcultured unlimitedly. Cells expanded in low-serum medium showed higher potential of mesenchymal differentiation. We suggest that high-serum concentration is unfavorable for self-renewing of stem cells due to growth factors contained in the serum. When SVF cells from patients at different ages were culture in high-serum medium, stem cells with osteogenic potential were depleted at ages above 20 years, whereas they could be expanded in low-serum medium even from 82 years old patient. Since human adult serum could substitute for 2% fetal bovine serum to give even better proliferation and differentiation, we could humanized ex vivo expansion culture of human stem cells for immediate clinical applications. By our method, autologous stem cell population corresponding to 10^9 cells can be prepared within 3 weeks after obtaining 1 g of subcutaneous adipose tissue and 200 ml of blood from patients at any age.

  • Research Products

    (9 results)

All 2006 2005 Other

All Journal Article (8 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] 脂肪組織からの体性幹細胞の分離2006

    • Author(s)
      北川泰雄, 他
    • Journal Title

      The Lipid 17・1

      Pages: 69-75

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ヒト脂肪組織由来幹細胞発見の経緯と臨床応用について2006

    • Author(s)
      北川泰雄, 他
    • Journal Title

      日本形成外科学会誌 (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Isolation of adult stem cells from adipose tissues. (in Japanese)2006

    • Author(s)
      Kitagawa Y., Kobori M., Toriyama K., Torii S.
    • Journal Title

      Lipids 17(1)

      Pages: 69-75

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Isolation of two human fibroblastic cell populations with multiple but distinct potential of mesenchymal differentiation by ceiling culture of mature fat cells from subcutaneous adipose tissue.2005

    • Author(s)
      Miyazaki Takamichi, et al.
    • Journal Title

      Differentiation 73・2-3

      Pages: 69-78

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Differential effect of green tea catechins on three endothelial cell clones isolated from rat adipose tissue and on human umbilical vein endothelial cells.2005

    • Author(s)
      Koparal, Tansu A., et al.
    • Journal Title

      Cytotechmology 46・1

      Pages: 25-36

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Isolation of two human fibroblastic cell populations with multiple but distinct potential of mesenchymal differentiation by ceiling culture of mature fat cells from subcutaneous adipose tissue2005

    • Author(s)
      Miyazaki T., Kitagawa Y., Toriyama K., Kobori M., Torii S.
    • Journal Title

      Differentiation 73(2-3)

      Pages: 69-78

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Differential effect of green tea catechins on three endothelial cell clones isolated from rat adipose tissue and on human umbilical vein endothelial cells.2005

    • Author(s)
      Koparal, T.A., Yamaguchi, H., Omae, K., Torii, S., Kitagawa, Y.
    • Journal Title

      Cytotechmology 46(1)

      Pages: 25-36

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Discovery of adipose-derived stem cells and their clinical application (in Japanese)

    • Author(s)
      Kitagawa Y., Kobori M., Toriyama K., Torii S., Kamei Y.
    • Journal Title

      Journal of Japanese Society for Plastic and Reconstructive Surgery. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Patent(Industrial Property Rights)] 低血清培地で選択的に増殖する動物組織偏在性の多分化性細胞2005

    • Inventor(s)
      北川泰雄 他7名
    • Industrial Property Rights Holder
      科学技術振興機構, 他3者
    • Industrial Property Number
      国際特許(全指定)PCT/JP2005/013100
    • Filing Date
      2005-07-08
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2007-12-13  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi