2005 Fiscal Year Final Research Report Summary
Roles of multifunctional adaptor proteins, GGAs, in membrane traffic
| Project/Area Number |
15209002
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
NAKAYAMA Kazuhisa Kyoto University, Graduate School of Pharmaceutical Sciences, Professor, 薬学研究科, 教授 (40192679)
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| Co-Investigator(Kenkyū-buntansha) |
SHIN Hye-Won Kyoto University, Graduate School of Pharmaceutical Sciences, Research associate, 薬学研究科, 研究員(科学研究) (10345598)
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| Project Period (FY) |
2003 – 2005
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| Keywords | Cells and tissues / Biomolecules / Proteins / Pharmaceutical sciences |
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| Research Abstract |
In this research project, we revealed the followings on GGAs and its related Tom1 family proteins.
(1)We found that ARFs bind to the N-GAT subdomain of the GAT domain of GGAs.
(2)By X-ray crystallography, we revealed the three-dimensional structure of the GGA3-GAT domain in complex with ARF.
(3)We found that ubiquitin bind to the C-GAT subdomain of the GAT domain of GGAs and Tom1 family proteins.
(4)By X-ray crystallography, we revealed the three-dimensional structure of the GGA3-GAT domain and that of the Tom1-GAT domain in complex with ubiquitin.
(5)We found that Tollip (Toll-interacting protein) interacts with the GAT domain of the Tom1 family proteins.
(6)We revealed that Tollip recruits Tom1 family proteins and ubiquitinated proteins onto endosomes.
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[Journal Article] Expression of BIG2 and analysis of its function in Mammalian Cells.2005
Author(s)
Shin, H.-W., Shinotsuka, C., Nakayama, K.
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Journal Title
Methods in Enzymology, GTPases regulating membrane dynamics (Balch, W.E., Der, C.J. & Hall, A., eds.)(Elsevier) Vol.404
Pages: 206-215
Description
「研究成果報告書概要(欧文)」より
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