2006 Fiscal Year Final Research Report Summary
Forensic Age-Estimation Using Proteomics-and Genomics-Approaches
| Project/Area Number |
15209023
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | University of Fukui (2004-2006)
福井医科大学 (2003) |
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Principal Investigator |
YASUDA Toshihiko University of Fukui, Faculty of Medical Sciences, Professor, 医学部, 教授 (80175645)
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| Co-Investigator(Kenkyū-buntansha) |
IIDA Reiko University of Fukui, Faculty of Medical Sciences, Assistant, 医学部, 助手 (40139788)
KAWAI Yasuyuki Kanazawa Medical University, Assistant, 医学部, 助手 (40324157)
UEKI Misuzu University of Fukui, Faculty of Medical Sciences, Instruction Official, 医学部, 教務職員 (00165656)
KOMINATO Yoshihiko Gunma University, Graduate School of Medicine, Professor, 大学院医学系研究科, 教授 (30205512)
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| Project Period (FY) |
2003 – 2006
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| Keywords | Forensic Science / Age-estimation / Genomics / Proteomics / Age-related alterations / Gene expression / Biomarkers / Deoxyribonuclease I |
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| Research Abstract |
1. It was clarified that the application of two non isotopic techniques as a genomics approach-fluorescence differential display-PCR to the first screening and the comparative RT-PCR to the subsequent confirmation-has allowed us to identify and characterize novel genes expressed in an age-related manner.
2. Using the method describe above, we succeeded in identification of an age-dependently expressed gene encoding a novel protein, Mpv17-like protein, M-LP in mouse kidney, then characterized its molecular-biological aspects such as the structure of cDNA and gene, and identification of spliced isoforms. Cell-biological analysis demonstrated that M-LP localized in peroxisomal membrane: we elucidated the peroxisomal membrane targeting signal and membrane topology of M-LP. Furthermore, M-LP may be involved in metabolism of reactive oxygen species through regulating expression of antioxidant enzyme genes.
3. We identified and characterized a human homolog of the M-LP as a marker fiage-estimat
… More
ion.
4. We succeeded in determination of the complete primary structure of a young age-specifi urinary glycoprotein, Ugl-Y, which had previously been identified by a proteomics approach Ugl-Y was clarified to be derived from a. young age-specific proteolysis of one of the matri proteins, fibronection.
5. Characterization of age-related alterations in the mitochondrial DNA (mtDNA) of various tissues revealed that mtDNA, its transcripts and mtDNA encoded products generally increase in content during the course of aging.
6. We have previously found levels of deoxyribonuclease I (DNase I) activity in human pituitary gland to alter in an age-related manner. An abrupt elevation of serum DNase I activity was observed within approximately 3 hours of the onset of acute myocardial infarction (AMI. Therefore, serum DNase I could be used as a new diagnostic marker for the early detection of AMI.
7. Transient myocardial ischemia during percutaneous coronary intervention induced a significant elevation of serum DNase I activity. Increased activity of the enzyme may prove useful as a sensitive marker for detection of transient myocardial ischemia.
8. Possible regulation of human DNase I gene expression, in which transcription factor Spl is involved, was elucidated. Less
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[Journal Article] Association of Gln222Arg polymorphism in the deoxyribonuclease I (DNase I) gene with myocardial infarction in Japanese patients.2006
Author(s)
T.Kumamoto, Y.Kawai, K.Arakawa, N.Morikawa, H.Tada, K.Taniguchi, R.Tatami, I.Miyamori, Y.Kominato, K.Kishi, T.Yasuda
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Journal Title
Eur. Heart J. 27(17)
Pages: 2081-2087
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Serum deoxyribonuclease I activity can be use as a sensitive marker for detection of transient myocardial ischemia induced by percutaneous coronary intervention.2005
Author(s)
K.Arakawa, K.Kawai, T.Kumamoto, N.Morikawa, M.Yoshida, H.Tada, R.Kawaguchi, K.Taniguchi, I.Miyamori, Y.Kominato, K.Kishi, T.Yasuda
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Journal Title
Eur. Heart J. 26(22)
Pages: 2375-2380
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Improvement of a multiplex PCR system for DYS441, DYS442, DYS443, DYS444 and DYS445, and a population study in 340 Japanese males.2005
Author(s)
Y.Ogata, T.Yamaba, K.Sawazaki, R.Iida, E.Tsuobota, H.Takatsuka, T.Matsuki, T.Yasuda, K.Kishi
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Journal Title
Legal Med. 7(3)
Pages: 183-189
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Forensic application of stimulous detection of five STRs (DYS441, DYS442, DYS443, DYS444 and DYS445) in Y-chromosome.2005
Author(s)
Y.Ogata, T.Yamaba, K.Sawazaki, R.Iida, H.Takatsuka, M.Masuyama, T.Matsuki, T.Yasuda, K.Kishi
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Journal Title
DNA Polymorphism 13
Pages: 204-206
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The diagnostic use of serum deoxyribonuclease I activity as a novel early-phase marker in acute myocardial infarction.2004
Author(s)
Y.Kawai, M.Yoshida, K.Arakawa, T.Kumamoto, N.Morikawa N.Tanaka, K.Masamura, H.Tada, S.Ito, H.Hoshizaki, S.Oshima, K.Taniguchi, H.Terasawa, I.Miyamori, K.Kishi, T.Yasuda
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Journal Title
Circulation 109(20)
Pages: 2398-2400
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A novel multiplex PCR system consisting of Y-STRs DYS441, DYS442, DYS443, DYS444 and DYS445.2003
Author(s)
R.Iida, K.Sawazaki, H.Ikeda, T.Miyamoto, E.Tsubota, H.Takatsuka, M.Masuyama, T.Matsuki, T.Yasuda, K.Kishi
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Journal Title
J. Forensic Sci. 48(5)
Pages: 1088-1090
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] An abrupt elevation of the serum deoxyribonuclease I (DNase I ) activity in acute myocardial infarction.2003
Author(s)
M.Yoshida, Y.Kawai, K.Masamura, M.Katoh, N.Tanaka, M.Saga, I.Miyamori, T.Yasuda, H.Tada, S.Ito, H.Hoshizaki, S.Oshima, K.Taniguchi
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Journal Title
Circulation J. 67(Suppl. 1)
Pages: 300
Description
「研究成果報告書概要(欧文)」より
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