2006 Fiscal Year Final Research Report Summary
Developmental analysis of neuro-immuno-endocirne network and harmonized histogenesis of organs
| Project/Area Number |
15209034
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Shimane University (2004-2006)
Shimane Medical University (2003) |
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Principal Investigator |
OTANI Hiroki Shimane University, Faculty of Medicine, Department of Developmental Biology, Professor, 医学部, 教授 (20160533)
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| Co-Investigator(Kenkyū-buntansha) |
HATTA Toshihisa Shimane University, Faculty of Medicine, Department of Developmental Biology, Associate Professor, 医学部, 助教授 (20238025)
HASHIMOTO Ryuju Shimane University, Faculty of Medicine, Department of Developmental Biology, Instructor, 医学部, 助手 (90252907)
MATSUMOTO Akihiro Shimane University, Faculty of Medicine, Department of Developmental Biology, Instructor, 医学部, 助手 (70346378)
UDAGAWA Jun Shimane University, Research Project Promotion Institute, Instructor, プロジェクト研究推進機構, 助手 (10284027)
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| Project Period (FY) |
2003 – 2006
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| Keywords | Neuro-immuno-endocirne network / histogenesis / mouse embryo / exo utero / leukemia inhibitory factor / adrenocorticotropic hormone / leptin |
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| Research Abstract |
Histogenesis of organs during mid-to late-gestation and early years after birth creates the basis of organ functions that work for whole life period, and thus variations in organ histogenesis are deeply related with predisposition of diseases involving these organs. In this study, we analyzed the development of neuro-immuno-endocrine network and its possible role in the 'harmonized' histogenesis of organs. By micro-injection and exo utero development system in mouse embryos, together with use of knockout and other genetically modified mice as well as in vitro experiments, effects of leukemia inhibitory factor (LIF), leptin, adrenocorticotropic hormone (ACTH), and other related humoral factors such as NPY and GM-CSF that are supposed to work in the network were examined in the brain and other organs of embryos. Novel roles of LIF and leptin have been elucidated in the cerebral histogenesis, and prenatal effects of ACTH on multiple organs have been suggested. LIF and other humoral factors were quantitatively analyzed in the serum, cerebrospinal fluid, and amniotic fluid of the embryo, and their levels were observed to change in relation to the development of organs. To further comprehensively analyze the 'harmonized' development of multiple organs in relation to histogenesis of each organ, we started to utilize mathematical methods. The pons in human fetuses and body parts of human embryos and fetuses were measured and analyzed with regard to developmental features. Also to analyze the possible effect of maternal environmental factors on histogenesis and predisposition of disease, non-obese diabetic (NOD) mice, an animal model of human type 1 diabetes, were analyzed by transferring embryos between NOD and other strain mice.
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