2005 Fiscal Year Final Research Report Summary
Exploration of molecular targets for diagnosis and therapy of oral cancer through array-CGH based analysis of cancer genome.
| Project/Area Number |
15209069
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
AMAGASA Teruo Tokyo Medical and Dental University, Graduate School, Maxillofacial Surgery, Professor, 大学院・医歯学総合研究科, 教授 (00014332)
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| Co-Investigator(Kenkyū-buntansha) |
INAZAWA Johji Tokyo Medical and Dental University, Medical Research Institute, Department of Molecular Cytogenetics, Professor, 難治疾患研究所, 教授 (30193551)
UZAWA Narikazu Tokyo Medical and Dental University, Graduate School, Maxillofacial Surgery, Research Associate, 大学院・医歯学総合研究科, 助手 (30345285)
IMOTO Issei Tokyo Medical and Dental University, Medical Research Institute, Department of Molecular Cytogenetics, Associate Professor, 難治疾患研究所, 助教授 (30258610)
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| Project Period (FY) |
2003 – 2005
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| Keywords | Oral cancer / Array-CGH / Genome / Molecular target / DNA methylation |
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| Research Abstract |
1) Banking of primary samples of oral cancer and its premalignant lesions with clinicopathological data
We made collections of cell lines as well as primary samples of oral cancer and its premalignant lesions with clinicopathological data for analyses of cancer genome of this disease.
2) Analysis of genomic copy-number aberration using array-comparative genomic hybridization (array-CGH) and identification of target gene
We analyzed genomic DNA from oral and esophageal cell lines using in-house array-based CGH (MCG Whole Genome Array-4500 and MCG Cancer Array-800). From the regions showing cryptic but remarkable genomic copy-number changes, such as high-level amplification and homozygous deletion, we tried to identify target genes for those aberrations. Together with further analyses using primary tumor samples, we successfully identified many cancer-related genes, including genes correlated with clinicopathological as well as biological significances.
3) Identification of tumor-suppressor genes silenced by DNA methylation using BAC-array-based methylated CpG-island amplification (BAMCA) and Chromatin immunoprecipitation (ChIP) -on-chip method
We combined MCA method to amplify methylated sequences with array-CGH (BAMCA), and screened aberrantly methylated sequences in cancer genome. We also combined ChIP with array-CGH (ChIP-on-chip) for screening of methylated sequences. Using those screening methods with following database search and expression analyses with and without pharmacological modifications, we successfully identified several genes silenced by methylation in esophageal and oral cancers. Among them, candidate tumor-suppressors frequently methylated in oral and esophageal cancers have been successfully identified (unpublished data)
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[Journal Article] Subcutaneous Injections of a TNF-α Antagonistic Peptide Inhibit Both Inflammation and Bone Resorption in Collagen-Induced Murine Arthritis.2005
Author(s)
Kojima T., Aoki K., Nonaka K., Saito H., Azuma M., Iwai H., Bobby J.Varghese., Yoshimasu H., Baron R., Ohya K., Amagasa T.
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Journal Title
J.Med.Dent.Sci 52(1)
Pages: 91-99
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] ADAM23, a possible tumor suppressor gene, is frequently silenced in gastric cancers by homozygous deletion or aberrant promoter hypermethylation.2005
Author(s)
Takada H., Imoto I., Tsuda H., Nakanishi Y., Ichikura T., Mochizuki H., Mitsufuji S., Hosoda F., Hirohashi S., Ohki M., Inazawa J
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Journal Title
Oncogene 24(54)
Pages: 8051-8060
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Array-based comparative genomic hybridization analysis of high-grade neuroendocrine tumors of the lung.2005
Author(s)
Peng WX., Shibata T., Katoh H., Kokubu A., Matsuno Y., Asamura H., Tsuchiya R., Kanai Y., Hosoda F., Sakiyama T., Ohki M., Imoto I., Inazawa J., Hirohashi S
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Journal Title
Cancer Sci. 96(10)
Pages: 661-667
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Genetic profile of hepatocellular carcinoma revealed by array-based comparative genomic hybridization : Identification of genetic indicators to predict patient outcome.2005
Author(s)
Katoh H., Shibata T., Kokubo A., Ojima H., Loukopoulos P., Kanai Y., Kosuge T., Fukayama M., Kondo T., Sakamoto M., Hosoda F., Ohki M., Imoto I., Inazawa J., Hirohashi S
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Journal Title
J Hepatol. 43(5)
Pages: 863-874
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Genetic classification of lung adenocarcinoma based on array-based comparative genomic hybridization analysis : its association with clinicopathologic features.2005
Author(s)
Shibata T., Uryu S., Kokubu A., Hosoda F., Ohki M., Sakiyama T., Matsuno Y., suchiya R., Kanai Y., Kondo T., Imoto I., Inazawa J., Hirohashi S
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Journal Title
Clin Cancer Res. 11(17)
Pages: 6177-6185
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Increase of bone marrow-derived secretory lineage epithelial cells during regeneration in the human intestine.2005
Author(s)
Matsumoto T., Okamoto R., Yajima T., Mori T., Okamoto S., Ikeda Y., Mukai M., Yamazaki M., Oshima S., Tsuchiya K., Nakamura T., Kanai T., Okano H., Inazawa J., Hibi T., Watanabe M
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Journal Title
Gastroenterology 128(7)
Pages: 1851-1867
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Diagnostic clinical application of two-color fluorescence in situ hybridization that detects chromosome 1 and 17 alterations to direct touch smear and liquid-based thin-layer cytologic preparations of endometrial cancers.2005
Author(s)
Susumu N., Aoki D., Noda T., Nagashima Y., Hirao T., Tamada Y., Banno K., Suzuki A., Tsuda H., Inazawa J., Nozawa S
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Journal Title
Int J Gynecol Cancer 15(1)
Pages: 70-80
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Correlation of KIT and EGFR overexpression with invasive ductal breast carcinoma of the solid-tubular subtype, nuclear grade 3, and mesenchymal or myoepithelial differentiation.2005
Author(s)
Tsuda H., Morita D., Kimura M., Shinto E., Ohtsuka Y., Matsubara O., Inazawa J., Takami K., Mochizuki H., Tamai S., Hiraide H
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Journal Title
Cancer Sci. 96(1)
Pages: 48-53
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The novel gene encoding a putative transmembrane protein is mutated in gnathodiaphyseal dysplasia (GDD).2004
Author(s)
Tsutsumi T., Kamata N., T.J.Vokes., Maruoka Y., Nakakuki K., Enomoto S., Omura K., Amagasa T., Nagayama M., Saito-Ohara F., Inazawa J., Moritani M., Yamaoka T., Inoue H., Itakura M.
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Journal Title
Am, J, Genet, 74
Pages: 1255-1261
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Alteration in copy numbers of genes as a mechanism for acquired drug resistance.2004
Author(s)
Yasui K., Mihara S., Zhao C., Okamoto H., Saito-Ohara F., Tomida A., Funato T., Yokomizo A., Naito S., Imoto I., Tsuruo T., Inazawa J
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Journal Title
Cancer Res. 64(4)
Pages: 1403-1410
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Mutations in EFHC1 cause juvenile myoclonic epilepsy.2004
Author(s)
Suzuki T., Delgado-Escueta AV., Aguan K., Alonso ME., Shi J., Hara Y., Nishida M., Numata T., Medina MT., Takeuchi T., Morita R., Bai D., Ganesh S., Sugimoto Y., Inazawa J., Bailey JN., Ochoa A., Jara-Prado A., Rasmussen A., Ramos-Peek J., Cordova S., Rubio-Donnadieu F., Inoue Y., Osawa M., Kaneko S., Oguni H., Mori Y., Yamakawa K
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Journal Title
Nat Genet. 36(8)
Pages: 842-849
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The novel gene encoding a putative transmembrane protein is mutated in gnathodiaphyseal dysplasia (GDD).2004
Author(s)
Tsutsumi S., Kamata N., Vokes TJ., Maruoka Y., Nakakuki K., Enomoto S., Omura K., Amagasa T., Nagayama M., Saito-Ohara F., Inazawa I., Moritani M., Yamaoka T., Inoue H., Itakura M
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Journal Title
Am J Hum Genet. 74(6)
Pages: 1255-1261
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Assessment of oral Transmission using cell-free human immunodeficiency virus-1 in mice reconstituted with human peripheral blood leucocyte.2003
Author(s)
Nakao R., Hanada N., Asano T., Hara T., MD.A.Saram., Matin K., Shimazu Y., Nakasone T., Horibata S., Aoba T., Honda M., Amagasa T., Senpuku H.
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Journal Title
Immunology 109
Pages: 271-282
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Chromosome arm 20q gains and other genomic alterations in esophageal squamous cell carcinoma., as analyzed by comparative genomic hybridization and fluorescence in situ hybridization.2003
Author(s)
Fujita Y., Sakakura C., Shimomura K., Nakanishi M., Yasuoka R., Aragane H., Hagiwara A., Abe T., Inazawa J., Yamagishi H
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Journal Title
Hepatogastroenterology 50(54)
Pages: 1857-1863
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Physical map and haplotype analysis of 16q-linked autosomal dominant cerebellar ataxia (ADCA) type III in Japan.2003
Author(s)
Li M., Ishikawa K., Toru S., Tomimitsu H., Takashima M., Goto J., Takiyama Y., Sasaki H., Imoto I., Imazawa J., Toda T., Kanazawa I., Mizusawa H
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Journal Title
J Hum Genet. 48(3)
Pages: 111-118
Description
「研究成果報告書概要(欧文)」より
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