2006 Fiscal Year Final Research Report Summary
Establishment of various measures against infectious diseases between domestic and wildlife animals, preventing the development of stock breeding in southern Africa
| Project/Area Number |
15255021
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Applied veterinary science
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| Research Institution | IWATE UNIVERSITY |
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Principal Investigator |
YASUDA Jun IWATE UNIV., FAC.AGRICULTURE, PROFESSOR, 農学部, 教授 (20142705)
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| Co-Investigator(Kenkyū-buntansha) |
ONUMA Misao HOKKAIDO UNIV.DISEASE CONTROL, GRADUATE SCHOOL VET.MED., PROFESSOR, 大学院・獣医学研究科, 教授 (70109510)
SUGIMOTO Chihiro HOKKAIDO UNIV., GRADUATE SCHOOL RESEARCH CENTER ZOONOSIS CONTROL, PROFESSOR, 大学院人獣共通感染症リサーチセンター, 教授 (90231373)
YOSHIDA Mitsutoshi KAGOSHIMA UNIV., FAC.AGRICULTURE, PROFESSOR, 農学部, 教授 (00174954)
FUKUSHI Hideto GIFU UNIV., FAC.AGRICULTURE, PROFESSOR, 応用生物科学部, 教授 (10156763)
ITAGAKI Tadashi IWATE UNIV., FAC.AGRICULTURE, PROFESSOR, 農学部, 教授 (80203074)
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| Project Period (FY) |
2003 – 2006
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| Keywords | ZAMBIA / CATTLE / WILD ANIMALS / HERPES VIRUS / SCHISTOMA / ANTI-TICK VACCINE / LAMP METHOD / SALMONELLA |
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| Research Abstract |
1.TRANSMISSIBLE DISEASES BETWEEN DOMESTIC AND WILD ANIMALS
Serological surveillance of BIV was conducted in traditional cattle in Zambia. BIV pol gene products showed 98.0-100% homology to the American strain. DNA sequences detected in springboks showed 44-72% homology to the Gammaherpesvirinae, indicating unrecognized novel virus. From nucleotide sequences of the VP2 hypervariable region of infectious bursal disease viruses in Tanzania, both African and European/Asian variants were demonstrated.
2.DEVELOPMENT OF ANTI-TICK VACCINE
Rapid amplification of the cDNA ends and primers designed based on a conserved seprin were used for the protease inhibitor from Haemaphysalis longicornis as an anti-tick vaccine. Vaccination of cattle with a combination of Rhipivcephalus appendiculutus seprine inhibitor conferred protective immunity against ticks. This is the first report on an anti-tick vaccine trial.
3.DEVELOPMENT OF NEW DIAGNOSTIC TECHNOLOGY
Species-specific loop-mediated isothermal amplification (LAMP) method is a novel nucleic acid method. We used this method for the diagnosis of equine piroplasmosis and bovine theileriosis and trypanosomosis. We tried a 2-step method using isothermal and chimeric primer-initiated amplification of nucleic acids to amplify the Salmonella invA and detecting the amplified product using ELISA from carcass samples in Zambia.
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