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2005 Fiscal Year Final Research Report Summary

Ultrasonic Nano-imaging of Vascular Endothelial Cell

Research Project

Project/Area Number 15300178
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Medical systems
Research InstitutionTohoku University

Principal Investigator

SAIJO Yoshifumi  Tohoku University, Institute of Development, Aging and Cancer, Associate Professor, 加齢医学研究所, 助教授 (00292277)

Co-Investigator(Kenkyū-buntansha) SATO Masaaki  Tohoku University, Graduate School of Engineering, Professor, 大学院・工学研究科, 教授 (30111371)
HOZUMI Naohiro  Toyohashi University of Technology, Graduate School of Engineering, Associate Professor, 大学院・工学研究科, 助教授 (30314090)
SUZUKI Satoshi  Tohoku University, Hospital, Lecturer, 病院・講師 (50344669)
Project Period (FY) 2003 – 2005
Keywordsacoustic microscopy / sound speed / attenuation / phase / ultrasonic tissue characterization / biomechanics / cell
Research Abstract

For precise imaging of cells, pre-treatments such as antibody sensing are needed in electron scanning microscopy and laser scanning microscopy with fluorescence technique. Non-contact nano-imaging of cells without pre-treatment is anticipated for repeated measurements under various conditions. The objective of the present research is to develop a nano-imaging system that can measure sound speed, attenuation and acoustic impedance of cells with high frequency ultrasound. Further, we compare the distribution of acoustic properties within a cell and cytoskeleton such as actin filaments or tubulin in order to prove the usefulness of the system in the measurement of cellular biomechanics.
An electric pulse with the pulse width of 2 ns was generated by high speed mechanical switching was directly input into the ultrasonic transducer. The pulse response was analyzed by a digital oscilloscope. Two servo linear motors controlled by a microcomputer board were used for the scanning in both x- and y-axis of the imaging system. The total system was controlled by a workstation and 300x300 points data acquisition was done within two minutes. The interference between reflections from the surface of the specimen and the interface between the specimen and a glass slide was analyzed in frequency domain. The algorithm for calculation of the thickness and the sound speed of the specimen was established and finally two-dimensional mapping of quantitative sound speed distribution was displayed.
Cooling effect of pulmonary vascular endothelial cells was investigated with actin filaments staining, electric impedance measurement and nano-imaging system. The results indicated the usefulness of the system for investigation of cellular biomechanics.

  • Research Products

    (38 results)

All 2005 2004 2003 2002

All Journal Article (35 results) Book (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Sound speed scanning acoustic microscopy for biomedical applications.2005

    • Author(s)
      Saijo Y et al.
    • Journal Title

      Technol Health Care 13・4

      Pages: 261-267

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Detection and correction of aliasing in ultrasonic measurement of blood flows with Ultrasonic-Measurement-Integrated simulation.2005

    • Author(s)
      Funamoto K et al.
    • Journal Title

      Technol Health Care 13・4

      Pages: 331-344

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Fundamental study of ultrasonic-measurement-integrated simulation of real blood flow in the aorta.2005

    • Author(s)
      Funamoto K et al.
    • Journal Title

      Ann Biomed Eng 33・4

      Pages: 415-428

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Influence of aging on cardiac function examined by echocardiography.2005

    • Author(s)
      Watanabe S et al.
    • Journal Title

      Tohoku J Exp Med 207・1

      Pages: 13-19

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Artificial esophagus with peristaltic movement.2005

    • Author(s)
      Watanabe M et al.
    • Journal Title

      ASAIO J 51-2

      Pages: 158-161

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Moment based texture segmentation of intravascular ultrasound images.2005

    • Author(s)
      Santos Filho E et al.
    • Journal Title

      J Med Ultrasonics 32・3

      Pages: 91-99

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Sound speed scanning acoustic microscopy for biomedical applications.2005

    • Author(s)
      Saijo Y et al.
    • Journal Title

      Technol Health Care 13-4

      Pages: 261-267

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Detection and correction of aliasing in ultrasonic measurement of blood flows with Ultrasonic-Measurement-Integrated simulation.2005

    • Author(s)
      Funamoto K et al.
    • Journal Title

      Technol Health Care 13-4

      Pages: 331-344

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Fundamental study of ultrasonic-measurement-integrated simulation of real blood flow in the aorta.2005

    • Author(s)
      Funamoto K et al.
    • Journal Title

      Ann Biomed Eng 33-4

      Pages: 415-428

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Influence of aging on cardiac function examined by echocardiography.2005

    • Author(s)
      Watanabe S et al.
    • Journal Title

      Tohoku J Exp Med 207-1

      Pages: 13-19

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Moment based texture segmentation of intravascular ultrasound images.2005

    • Author(s)
      Santos Filho E et al.
    • Journal Title

      J Med Ultrasonics 32-3

      Pages: 91-99

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Acoustic properties of aortic aneurysm obtained with scanning acoustic microscopy.2004

    • Author(s)
      Saijo Y et al.
    • Journal Title

      Ultrasonics 42・1-9

      Pages: 695-698

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Development of an ultra-portable echo device connected to USB port.2004

    • Author(s)
      Saijo Y et al.
    • Journal Title

      Ultrasonics 42・1-9

      Pages: 699-703

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Time- frequency analysis for pulse driven ultrasonic microscopy for biological tissue characterization.2004

    • Author(s)
      Hozumi N et al.
    • Journal Title

      Ultrasonics 42・1-9

      Pages: 717-722

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Tissue velocity imaging of coronary artery by rotating-type intravascular ultrasound.2004

    • Author(s)
      Saijo Y et al.
    • Journal Title

      Ultrasonics 42・1-9

      Pages: 753-757

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Paclitaxel prevents loss of pulmonary endothelial barrier integrity during cold preservation.2004

    • Author(s)
      Suzuki S et al.
    • Journal Title

      Transplantation 78・4

      Pages: 524-529

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Acoustic and mechanical properties of aortic aneurysms assessed by scanning acoustic microscopy.2004

    • Author(s)
      Saijo Y et al.
    • Journal Title

      Acoustical Imaging 27

      Pages: 479-483

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Acoustic properties of aortic aneurysm obtained with scanning acoustic microscopy.2004

    • Author(s)
      Saijo Y et al.
    • Journal Title

      Ultrasonics 42-1-9

      Pages: 695-698

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Development of an ultra-portable echo device connected to USB port.2004

    • Author(s)
      Saijo Y et al.
    • Journal Title

      Ultrasonics 42-1-9

      Pages: 699-703

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Time-frequency analysis for pulse driven ultrasonic microscopy for biological tissue characterization.2004

    • Author(s)
      Hozumi N et al.
    • Journal Title

      Ultrasonics 42-1-9

      Pages: 717-722

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Tissue velocity imaging of coronary artery by rotating-type intravascular ultrasound.2004

    • Author(s)
      Saijo Y et al.
    • Journal Title

      Ultrasonics 42-1-9

      Pages: 753-757

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Paclitaxel prevents loss of pulmonary endothelial barrier integrity during cold preservation.2004

    • Author(s)
      Suzuki S et al.
    • Journal Title

      Transplantation 78-4

      Pages: 524-529

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Acoustic and mechanical properties of aortic aneurysms assessed by scanning acoustic microscopy.2004

    • Author(s)
      Saijo Y et al.
    • Journal Title

      Acoustical Imaging (W.Arnold and S.Hirsekorn eds.) (Kluwer Academic Publishers) 27

      Pages: 479-483

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Fast Fourier transform acoustic microscopy for biological tissues.2003

    • Author(s)
      Saijo Y et al.
    • Journal Title

      Ultrasound Med Biol 29・5s

      Pages: S20

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Pocket-size echo connectable to a personal computer.2003

    • Author(s)
      Saijo Y et al.
    • Journal Title

      Ultrasound Med Biol 29・5s

      Pages: S59

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Intravascular tissue velocity imaging of coronary artery.2003

    • Author(s)
      Saijo Y et al.
    • Journal Title

      Ultrasound Med Biol 29・5s

      Pages: S173

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Influence of tissue preparation on the acoustic properties of tissue sections at high frequencies.2003

    • Author(s)
      Saijo Y et al.
    • Journal Title

      Ultrasound Med Biol 29・9

      Pages: 1367-72

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 超音波による心血管系の組織診断2003

    • Author(s)
      西條芳文
    • Journal Title

      加齢医学研究所雑誌 54・3

      Pages: 93-105

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 高周波数超音波による心血管系の組織診断2003

    • Author(s)
      西條芳文
    • Journal Title

      Med Imag Tech 21・2

      Pages: 101-105

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Fast Fourier transform acoustic microscopy for biological tissues.2003

    • Author(s)
      Saijo Y et al.
    • Journal Title

      Ultrasound Med Biol 29-5S

      Pages: S20

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Pocket-size echo connectable to a personal computer.2003

    • Author(s)
      Saijo Y et al.
    • Journal Title

      Ultrasound Med Biol 29-5S

      Pages: S54

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Intravascular tissue velocity imaging of coronary artery.2003

    • Author(s)
      Saijo Y et al.
    • Journal Title

      Ultrasound Med Biol 29-5S

      Pages: S173

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Influence of tissue preparation on the acoustic properties of tissue sections at high frequencies.2003

    • Author(s)
      Sasaki H et al.
    • Journal Title

      Ultrasound Med Biol 29-9

      Pages: 1367-1372

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Ultrasonic tissue characterization of cardiovascular tissues. (in Japanese)2003

    • Author(s)
      Saijo Y.
    • Journal Title

      Kareiigaku Kenkyuusho Zasshi 54-3

      Pages: 93-105

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Tissue characterization of cardiovascular tissues by high frequency ultrasound. (in Japanese)2003

    • Author(s)
      Saijo Y.
    • Journal Title

      Med Imag Tech 21-2

      Pages: 101-105

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Vascular Ultrasound2003

    • Author(s)
      Saijo Y et al.
    • Total Pages
      328
    • Publisher
      Springer-Verlaq
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Vascular Ultrasound2003

    • Author(s)
      Saijo Y, van der Steen AFW eds.
    • Total Pages
      328
    • Publisher
      Springer Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
  • [Patent(Industrial Property Rights)] 超音波診断装置2002

    • Inventor(s)
      西條芳文, 田中明, 坪根泉, 関水英正
    • Industrial Property Rights Holder
      西條芳文, フクダ電子株式会社
    • Industrial Property Number
      特許 第3720797号
    • Filing Date
      2002-08-26
    • Acquisition Date
      2005-09-16
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2007-12-13   Modified: 2021-09-01  

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