2004 Fiscal Year Final Research Report Summary
Isolation and Characterization of A Novel Human Radio-Susceptibility Gene, hNp95
| Project/Area Number |
15310044
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | National Institute of Radiological sciences |
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Principal Investigator |
FUJIMORI Akira National Institute of Radiological sciences, Research Center for Radiation Safety, Researcher, 放射線安全研究センター, 研究員 (50314183)
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| Co-Investigator(Kenkyū-buntansha) |
TATSUMI Kouichi National Institute of Radiological sciences, Research Center for Radiation Safety, Researcher, 放射線安全研究センター, 研究員 (30131022)
MUTO Masahiro National Institute of Radiological sciences, Research Center for Radiation Safety, Researcher, 放射線安全研究センター, 研究員 (80166230)
TAHAHAGI Masahiko National Institute of Radiological sciences, Research Center for Radiation Safety, Researcher, 放射線安全研究センター, 研究員 (30260235)
KUBO Eiko National Institute of Radiological sciences, Research Center for Radiation Safety, Researcher, 放射線安全研究センター, 研究員 (40161658)
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| Project Period (FY) |
2003 – 2004
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| Keywords | DNA binding protein / DNA damage / nuclear protein / DNA replication |
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| Research Abstract |
Murine nuclear protein NP95 has been shown to underlie resistance to ionizing radiation and other DNA insults or replication arrests in embryonic stem (ES) cells. Using the databases for expressed sequenced tags and a two-step PCR procedure, we isolated hNp95, the full-length human homologue of the murine NP95 cDNA, with a single open reading frame (ORF) encoding a polypeptide of 793 amino acids of 73.3% homology to NP95. The ORF of the hNP95 cDNA is identical to UHRF1 and only differs by two amino acids and seven nucleotides from that of the inverted CCAAT box binding protein of 90 kDa (ICBP90) that has been reported to regulate the transcription of the topoisomerase II gene. The hNp95 gene, assigned to 19p13.3, consists of 18 exons, spanning 60 kb, whereas the ICBP90 gene has been reported to comprise 6 exons. Several stable transformants from HEK293 and WI-38 cells that had been transfected with the antisense hNP95 cDNA were more sensitive to X-rays, UV-light and hydroxyurea than corresponding parental cells. hNP95 loss did not affect the activities of topoisomerase II□in HEK293 cells. These findings collectively indicate that the hNp95 gene is the human functional orthologue of the murine Np95 gene.
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[Journal Article] Isolation and Characterization of A Novel Human Radio-Susceptibility Gene, hNp952006
Author(s)
Masahiro Muto, Akira Fujimori, Mituru Nenoi, Kazuhiro Daino, Yoichi Matsuda, Asato Kuroiwa, Eiko Kubo, Yasuyoshi Kanari, Makoto Utsuno, Hideo Tsuji, Hideki Ukai, Kazuei Mita, Kouichi Tatsumi
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Journal Title
Radiation Research (in press)
Description
「研究成果報告書概要(和文)」より
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